LOCUS       sequence-371435-        7537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for integrating nonTATAchr21 promoter-driven dual 
            surface marker/mCitrine fluorescent reporter at AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pCL051
            AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9s...
FEATURES             Location/Qualifiers
     source          1..7537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     432..451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     685..702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    819..840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(855..860,861..878,879..885)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    893..909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     898..920
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     917..933
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     917..933
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        954..972
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1007..1810
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1817..1842
                     /label=SA
                     /note="splice acceptor site"
     CDS             1866..1919
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1929..2528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1929..1948)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2425..2445
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2560..2577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2566..2790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2863..2881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2899..2917
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2935..2953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2971..2989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3007..3025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3043..3061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3115..3133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3151..3169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      3352..3361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3358..3417
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             4189..4218
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4222..4368
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4384..4437
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4444..4453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4450..4452,4453..4455,4456..5166)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4495..4516)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4756..4775)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5103..5124
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5167..5187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5198..5319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5235..5254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5289..5308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5385..6221
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(6496..7287,7288..7356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7119..7138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7480..7537,1..531)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
       61 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cagaattaac
      961 cctcactaaa gggactagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc
     1021 agcattctct cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct
     1081 ctacggctgg cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc
     1141 tcttcccctt gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt
     1201 ctcggcgctg caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc
     1261 gggcatctct cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc
     1321 ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt
     1381 ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa
     1441 ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc
     1501 tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc
     1561 ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct
     1621 tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt
     1681 caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata
     1741 ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc
     1801 acagtggggc aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca
     1861 gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     1921 ggctcgagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     1981 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     2041 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     2101 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     2161 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     2221 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     2281 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     2341 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     2401 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     2461 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     2521 gtgcctgatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2761 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctgggggc
     2821 tagcaaagcc taggaacagc tgcatatagc actgcagttt actccctatc agtgatagag
     2881 agtcaaaagg ccatgccctc cctatcagtg atagagagag cagactcaag gtggtcccta
     2941 tcagtgatag agagattgag caagagggcc tccctatcag tgatagagaa tagcactgca
     3001 gtttactccc tatcagtgat agagagtcaa aaggccatgc cctccctatc agtgatagag
     3061 agagcagact caaggtggtc cctatcagtg atagagagat tgagcaagag ggcctcccta
     3121 tcagtgatag agattgatcc agtcagggcc tccctatcag tgatagagaa gctatgtgca
     3181 ggtagggctg tacagtggga atgcatgatg gcgtcacaaa ggcgccgcgg ctcgcagcgc
     3241 gctctgggag tgagcgcggc agtggccctg gcagctggcg gagcggccca gcccgtgtgc
     3301 acgtggcgcc ggcgctgcct ttcggttgcc aaaattcagc caccgcggtg ggccaccatg
     3361 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
     3421 gctagcgaga cgggcggatc aggcggtcgt ctcggatccg acaaaactca cacatgccca
     3481 ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc
     3541 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
     3601 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
     3661 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc
     3721 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc
     3781 ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag
     3841 gtctacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc
     3901 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
     3961 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
     4021 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg
     4081 atgcacgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa
     4141 ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggaga acaaaaactc
     4201 atctcagaag aggatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca
     4261 cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc
     4321 atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgttg tacaggcagt
     4381 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg
     4441 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4501 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4561 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4621 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
     4681 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4741 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4801 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4861 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4921 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4981 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5041 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5101 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5161 tacaagccaa aaaagaagag aaaggtatag ttaactaaac ttgtttattg cagcttataa
     5221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgca tatgtaaatt
     5341 gtaagcgtct cggcctctag agtcgagatg acattgatta ttgaactagg gacaggattg
     5401 gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa
     5461 cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca
     5521 tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg
     5581 agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca
     5641 gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg
     5701 cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg
     5761 aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt
     5821 ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca
     5881 gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt
     5941 gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc
     6001 tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga
     6061 gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc
     6121 ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg
     6181 ctctcctgcc ccttccctac aggggttcct ggctctgctc ttcgccttct tgacgagttc
     6241 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     6301 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6361 tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt
     6421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7021 cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag aatagtgtat
     7081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7381 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
     7441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7501 cgtaatctgc tgcttgcaaa caaaaaaacc accgcta
//
