LOCUS       sequence-371436-        7667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for integrating RSV promoter-driven dual surface 
            marker/mCitrine fluorescent reporter at AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pCL025
            AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIg...
FEATURES             Location/Qualifiers
     source          1..7667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     432..451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     685..702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    819..840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(855..860,861..878,879..885)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    893..909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     898..920
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     917..933
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     917..933
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        954..972
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1007..1810
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1817..1842
                     /label=SA
                     /note="splice acceptor site"
     CDS             1866..1919
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1929..2528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1929..1948)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2425..2445
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2560..2577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2566..2790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2863..2881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2899..2917
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2935..2953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2971..2989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3007..3025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3043..3061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3115..3133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3151..3169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3207..3469
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      3482..3491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3488..3547
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             4319..4348
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4352..4498
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4514..4567
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4574..4583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4580..4582,4583..4585,4586..5296)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4625..4646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4886..4905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5233..5254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5297..5317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5328..5449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5365..5384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5419..5438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5515..6351
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(6626..7417,7418..7486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7249..7268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7610..7667,1..531)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
       61 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cagaattaac
      961 cctcactaaa gggactagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc
     1021 agcattctct cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct
     1081 ctacggctgg cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc
     1141 tcttcccctt gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt
     1201 ctcggcgctg caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc
     1261 gggcatctct cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc
     1321 ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt
     1381 ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa
     1441 ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc
     1501 tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc
     1561 ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct
     1621 tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt
     1681 caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata
     1741 ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc
     1801 acagtggggc aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca
     1861 gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     1921 ggctcgagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     1981 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     2041 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     2101 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     2161 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     2221 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     2281 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     2341 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     2401 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     2461 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     2521 gtgcctgatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2761 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctgggggc
     2821 tagcaaagcc taggaacagc tgcatatagc actgcagttt actccctatc agtgatagag
     2881 agtcaaaagg ccatgccctc cctatcagtg atagagagag cagactcaag gtggtcccta
     2941 tcagtgatag agagattgag caagagggcc tccctatcag tgatagagaa tagcactgca
     3001 gtttactccc tatcagtgat agagagtcaa aaggccatgc cctccctatc agtgatagag
     3061 agagcagact caaggtggtc cctatcagtg atagagagat tgagcaagag ggcctcccta
     3121 tcagtgatag agattgatcc agtcagggcc tccctatcag tgatagagaa gctatgtgca
     3181 ggtagggctg tacagtggga atgcataatg tagtcttatg caatacactt gtagtcttgc
     3241 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     3301 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacaggtctg
     3361 acatggattg gacgaaccac tgaattgcgc attgcagaga taattgtatt taagtgccta
     3421 gctcgataca ataaacgcca tttgaccatt caccacattg gtgtgcaccc caccgcggtg
     3481 ggccaccatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     3541 cactggtgac gctagcgaga cgggcggatc aggcggtcgt ctcggatccg acaaaactca
     3601 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
     3661 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
     3721 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
     3781 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
     3841 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
     3901 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
     3961 agaaccacag gtctacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag
     4021 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
     4081 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
     4141 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
     4201 atgctccgtg atgcacgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc
     4261 tccgggtaaa ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggaga
     4321 acaaaaactc atctcagaag aggatctgaa tgctgtgggc caggacacgc aggaggtcat
     4381 cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt
     4441 ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgttg
     4501 tacaggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     4561 tggcccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4621 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4681 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4741 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg
     4801 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4861 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4921 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4981 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5041 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5101 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5161 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     5221 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5281 ggacgagctg tacaagccaa aaaagaagag aaaggtatag ttaactaaac ttgtttattg
     5341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgca
     5461 tatgtaaatt gtaagcgtct cggcctctag agtcgagatg acattgatta ttgaactagg
     5521 gacaggattg gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata
     5581 ttgggtctaa cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt
     5641 cctggagcca tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga
     5701 ggatcctggg agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc
     5761 ccggttctca gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg
     5821 ctgtctggtg cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga
     5881 agcagtttgg aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca
     5941 cctttctagt ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg
     6001 ccagtagcca gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa
     6061 ctccctttgt gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc
     6121 tttctctttc tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc
     6181 tccgcccaga gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc
     6241 cttggcaagc ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc
     6301 atgcccctgg ctctcctgcc ccttccctac aggggttcct ggctctgctc ttcgccttct
     6361 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac
     6421 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
     6481 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa
     6541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7141 tcatgccatc cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag
     7201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7501 aatattattg aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt
     7561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgcta
//
