LOCUS       sequence-371442-        8146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination donor plasmid for CRISPR/Cas9 targeting of 
            GFP fusion protein to the stop codon of endogenous human ABCA3 gene 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Sun YL, Hurley K, Villacorta-Martin C, Huang J, Hinds A, Gopalan K, 
            Caballero IS, Russo SJ, Kitzmiller JA, Whitsett JA, Beers MF, Kotton
            DN
  TITLE     Heterogeneity in Human Induced Pluripotent Stem Cell-derived 
            Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC 
            Reporters.
  JOURNAL   Am J Respir Cell Mol Biol. 2021 Oct;65(4):442-460. doi: 
            10.1165/rcmb.2020-0259OC.
  PUBMED    34101541
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1165/rcmb"; 
            journalName: "Am J Respir Cell Mol Biol"; date: "2021-10"; volume: 
            "65"; issue: "4"; pages: "442-460"
COMMENT     Sequence Label: phABCA3-eGFP_CRISPR_Donor
FEATURES             Location/Qualifiers
     source          1..8146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1011..1013,1014..1016,1017..1730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1011..1013,1014..1016,1017..1727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1056..1077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1317..1336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1664..1685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1777..1794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1783..2007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2030..2063
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2124..2622
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2159..2182)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2555..2574
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2662..3261
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2662..2681)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    3818..3851
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4579..4598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4580..4598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4605..4622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4605..4621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4614..4636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4762..5190
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4849..4868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5059..5080
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5534..6328
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5588..5607)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6198..6217
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6346..6414,6415..7206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6564..6583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7377..7965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7866..7885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8119..8136
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 cagggtggct cacaccataa tcccagcact ttgggaggcc gaggagggag gattgcttaa
      361 gcctgggagt tctagaccag cctgggcaac atagaccccg tctctacaaa aaatttaaaa
      421 attagccagg tatcatgcat acctgtagtc ccagccactc gggaggctaa ggtgggagga
      481 tcgcttgagc ctgggagttc gagactgcag tgagctatga ttgtgccact gcactccagc
      541 ctgggctaca gagcaagact ctctctaaaa aaatatgggg gaaaaaaaga actggctcag
      601 aaagggaaca tcactggctc aaggttgtaa tctgagctgg gaacagcaca gggaggaggc
      661 tgggccccat ccccgctggc tcctgcttag ccagcgtcct ctacctggga gcacggtgcc
      721 gtcgcagggc tgacctctct ggggctctgg ggtggcgctg ggcaggggta ggattgtggg
      781 ggcctcatca gcccctattg ccagaggact cccaggtccc gcagaggagc ctgggccctg
      841 ttctctccta ccagtgactt ttctgctccc caaaggtttt cggtattctg gagaaagcca
      901 aggaaaagta cggcgtggac gactactccg tgagccagat ctcgctggaa caggtcttcc
      961 tgagcttcgc ccacctgcag ccgcccaccg cagaggaggg gcgaccggtc atggtgagca
     1021 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1081 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1141 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1201 ccctgaccta cggcgtgcag tgcttcagtc gctaccccga ccacatgaag cagcacgact
     1261 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1321 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1381 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1441 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1501 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1561 agcagaacac ccccatcggc gacggccccg tgctgctgcc tgacaaccac tacctgagca
     1621 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1681 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     1741 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1801 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1861 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1921 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1981 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccaga taacttcgta
     2041 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag
     2101 gatctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2161 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     2221 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     2281 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     2341 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     2401 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     2461 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     2521 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     2581 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat
     2641 ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     2701 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     2761 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     2821 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     2881 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     2941 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     3001 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     3061 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     3121 gccggggtgc ccgccttcct ggagacctcc gcgccccaca acctcccctt ctacgagcgg
     3181 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     3241 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     3301 gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga tgatctatta
     3361 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
     3421 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
     3481 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
     3541 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
     3601 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     3661 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
     3721 agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa
     3781 ttccatcaga agctggtcga gatccggaac ccttaatata acttcgtata atgtatgcta
     3841 tacgaagtta ttaggtccct cgaagaggtt cactaggcgc gccatcttac atcctctctc
     3901 tccaagttta tctcatcctt tatttttaat cacttttttc tatgatggat atgaaaaatt
     3961 caaggcagta tgcacagaat ggacgagtgc agcccagccc tcatgcccag gatcagcatg
     4021 cgcatctcca tgtctgcata ctctggagtt cactttccca gagctggggc aggccgggca
     4081 gtctgcgggc aagctccggg gtctctgggt ggagagctga cccaggaagg gctgcagctg
     4141 agctgggggt tgaatttctc caggcactcc ctggagagag gacccagtga cttgtccaag
     4201 tttacacacg acactaatct cccctgggga ggaagcggga agccagccag gttgaactgt
     4261 agcgaggccc ccaggccgcc aggaatggac catgcagatc actgtcagtg gagggaagct
     4321 gctgactgtg attaggtgct ggggtcttag cgtccagcgc agcccggggg catcctggag
     4381 gctctgctcc ttagggcatg gtagtcaccg cgaagccggg caccgtccca cagcatctcc
     4441 tagaagcagc cggcacagga gggaaggtgg ccaggctcga agcagtctct gtttccagcg
     4501 gccggccaaa agggcgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca
     4561 tctagagggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     4621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4741 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4801 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4861 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4921 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4981 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5041 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5101 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5161 agctgattta acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta
     5221 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     5281 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     5341 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     5401 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     5461 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     5521 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5581 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5641 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5701 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5761 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5821 agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat
     5881 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5941 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6001 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6061 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6121 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6181 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6241 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6301 ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat
     6361 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6421 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6481 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6541 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6601 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6661 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6721 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6781 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6841 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6901 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6961 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7021 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7081 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7141 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7201 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7261 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7501 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8041 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8101 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
