LOCUS       sequence-371445-        9519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of Zyxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Hoffman LM, Jensen CC, Chaturvedi A, Yoshigi M, Beckerle MC
  TITLE     Stretch-induced actin remodeling requires targeting of zyxin to 
            stress fibers and recruitment of actin regulators.
  JOURNAL   Mol Biol Cell. 2012 May;23(10):1846-59. doi: 
            10.1091/mbc.E11-12-1057. Epub 2012 Mar 28.
  PUBMED    22456508
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-12-1057"; journalName: "Mol Biol Cell"; date: 
            "2012-05"; volume: "23"; issue: "10"; pages: "1846-59"
COMMENT     Sequence Label: pLenti-Zyxin-B (aa309-564)-EGFP
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3367..3387
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(3390..3392,3393..3395,3396..4106)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3390..3392,3393..3395,3396..4106)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3435..3456)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3696..3715)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4043..4064
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4160..4178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4183..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4380..4404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4457..4498
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4522..5110
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4575..4595)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4993..5004)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5125..5441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5292..5427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5489..5536
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5555..5953
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6040..6273
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6345..6466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6506..6641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6662..6680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6690..6707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6690..6706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6848..7303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6935..6954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7145..7166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7329..7433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7434..7502,7503..8294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7652..7671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8465..9053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8954..8973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9207..9224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9341..9362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9377..9382,9383..9400,9401..9407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9415..9431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9420..9442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9439..9455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9439..9455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9474..9494
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9476..9494
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg cttaatggct cagcagaagg agaagcccct agttcaagag aagcagcacc
     2641 cacagcctcc accagctcaa aaccaaaacc aggtacgctc tcctggaggc ccaggcccct
     2701 tgaccctgaa ggaggtagag gagttggagc agctgaccca gcagctgatg caggacatgg
     2761 aacaccctca gaggcagagc gtggcagtga atgagtcctg tggcaaatgc aatcagccac
     2821 tggcccgtgc acagcctgcg gttcgtgcac tgggacaact gttccacatc acctgcttca
     2881 cttgccatca gtgtcagcag cagctgcagg gacagcagtt ctatagcctg gagggagcac
     2941 catattgtga gggctgctac accgacactt tggagaagtg caacacctgt gggcagccca
     3001 tcactgaccg catgctgagg gccactggca aagcctacca cccacagtgc ttcacctgtg
     3061 tggtctgcgc ctgtcccctg gagggcacct ccttcattgt ggaccaggcc aatcagcccc
     3121 actgtgtccc tgactatcac aagcaatacg ctccaaggtg ctccgtctgc tcggagccaa
     3181 tcatgcctga gcctggccga gacgagactg tgcgagtagt ggcgctggat aagaactttc
     3241 atatgaagtg ttacaagtgt gaggactgtg ggaaacctct gtccattgag gcagatgaca
     3301 acggctgttt ccctctggat ggccacgtcc tttgtcggaa gtgccactcc gctagagccc
     3361 agaccacaac tttgtataca aaagttgtga tggtgagcaa gggcgaggag ctgttcaccg
     3421 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3481 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3541 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3601 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3661 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3721 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3781 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3841 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3901 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3961 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4021 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4081 ctctcggcat ggacgagctg tacaagggcg gtggaagatc tgggaattca aggcctctcg
     4141 agcctctaga ttctgcagcc ctatagtgag tcgtattacg tagatccaga catgataaga
     4201 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4261 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4321 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttta
     4381 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga
     4441 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     4501 taccggttag taatgatcga caatcaacct ctggattaca aaatttgtga aagattgact
     4561 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4621 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4681 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4741 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4801 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4861 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     4921 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4981 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5041 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5101 gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5161 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5221 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5281 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5341 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5401 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5461 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     5521 catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga
     5581 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     5641 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     5701 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     5761 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     5821 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     5881 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     5941 gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     6001 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6061 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6121 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6181 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6241 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6301 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6481 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6661 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6721 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6781 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6841 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7261 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8581 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8821 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9301 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9361 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9421 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9481 accctcacta aagggaacaa aagctggagc tgcaagctt
//
