LOCUS       sequence-371447-        2630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 2 plasmid to introduce the CVB3 IRES into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: CVB3 IRES
FEATURES             Location/Qualifiers
     source          1..2630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..127)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      230..818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     719..738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1792..2583,2584..2630,1..22))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2415..2434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
       61 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      121 ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct taacgtgagt
      181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      901 attctgtgga taaccgtgcg gccgctcggt ctcatatctt aaaacagcct gtgggttgat
      961 cccacccaca ggcccattgg gcgctagcac tctggtatca cggtaccttt gtgcgcctgt
     1021 tttatacccc ctcccccaac tgtaacttag aagtaacaca caccgatcaa cagtcagcgt
     1081 ggcacaccag ccacgttttg atcaagcact tctgttaccc cggactgagt atcaatagac
     1141 tgctcacgcg gttgaaggag aaagcgttcg ttatccggcc aactacttcg aaaaacctag
     1201 taacaccgtg gaagttgcag agtgtttcgc tcagcactac cccagtgtag atcaggtcga
     1261 tgagtcaccg cattccccac gggcgaccgt ggcggtggct gcgttggcgg cctgcccatg
     1321 gggaaaccca tgggacgctc taatacagac atggtgcgaa gagtctattg agctagttgg
     1381 tagtcctccg gcccctgaat gcggctaatc ctaactgcgg agcacacacc ctcaagccag
     1441 agggcagtgt gtcgtaacgg gcaactctgc agcggaaccg actactttgg gtgtcgtgtt
     1501 tcattttatt cctatactgg ctgcttatgg tgacaattga gagatcgtta ccatatagct
     1561 attggattgg ccatccggtg actaatagag ctattatata tccctttgtt gggtttatac
     1621 cacttagctt gaaagaggtt aaaacattac aattcattgt taagttgaat acagcaaaat
     1681 ggtgagaccg cggccgcgat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1921 aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
//
