LOCUS       sequence-371450-        2516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: HRV-B3 IRES
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     476..495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1435..2226,2227..2295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2058..2077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2296..2400)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2503..2516,1..575)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
       61 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      661 ctgtggataa ccgtgcggcc gctcggtctc atatcttaaa acagcggatg ggtaccccac
      721 catccgaccc actgggtgta gtactctggt acttcgtacc tttgtacgcc tgttcttccc
      781 attgtaccct tcctgaactt ccaacccaag taacgttaga agctcaacat ttagtacaac
      841 aggaagcacc acatccagtg gtgtttagta caagcacttc tgtttccccg gagcgaggta
      901 taggctgtac ccactgccaa aaacctttaa ccgttatccg ccaaccaact acgtaaaagc
      961 tagtagtatt atgtttttaa ctaggcgttc gatcaggtgg atttcccctc cactagtttg
     1021 gtcgatgagg ctaggaattc cccacgggtg accgtgtcct agcctgcgtg gcggccaacc
     1081 cagcttatgc tgggacgcct ttttatagac atggtgtgaa gactcgcatg tgcttggttg
     1141 tgattcctcc ggcccctgaa tgcggctaac cttaaccctg gagccttgtg tcacaaacca
     1201 gtgatgataa ggtcgtaatg agcaattccg ggacgggacc gactactttg ggtgtccgtg
     1261 tttcttattt ttcttattat tgtcttatgg tcacagcata tatataacat atactgtgat
     1321 catggtgaga ccgcggccgc gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1561 tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2401 cacatttccc cgaaaagtgc cacctgtcat gaccaaaatc ccttaacgtg agttttcgtt
     2461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc cttttt
//
