LOCUS       sequence-371453-        2226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream
            intron into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: HBA1 full 3'UTR-T4 td downstream intron
FEATURES             Location/Qualifiers
     source          1..2226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(998..1789,1790..1858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1621..1640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1859..1963)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2066..2226,1..428)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
       61 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      481 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgctcggt
      541 ctcatagtaa caattggctg gagcctcggt ggccatgctt cttgcccctt gggcctcccc
      601 ccagcccctc ctccccttcc tgcacccgta cccccgtggt ctttgaataa agtctgaacc
      661 acacaaatgg tcgccgactc agtagatgtt ttcttgggtt aattgaggcc tgagtataag
      721 gtgacttata cttgtaatct atctaaacgg ggaacctctc tagtagacaa tcccgtgcta
      781 aattgtagga ctgcccttta ataaatactt ctatatttaa agaggtattt atgaaaagcg
      841 gaatttatca gattaaaaat actttctcta gagtcgacct gcagacgctg agaccgcggc
      901 cgcgattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      961 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1021 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1081 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg
     1141 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1201 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1261 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1321 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1381 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1441 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1501 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1561 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1621 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1681 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1741 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1801 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1861 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1921 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1981 tgccacctgt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2221 tgtagc
//
