LOCUS       sequence-37147-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1307
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(157..175)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        254..358
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(577..596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgccttgct caccatggtg gcggaagttc ctataccttt tgaagaatag gaacttcgga
       61 ataggaactt cggcgcgcca cgcgtggccg gcctaggagc tcgaattcat taagggttat
      121 tgaatatgat cggaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      181 aggttaatgt catgataata atggtttctt agacgtgcgg ccgccgtcag gtggcacttt
      241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      481 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      541 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      601 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      661 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      721 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      781 aggaccgaag gagctaa
//
