LOCUS       sequence-371485-        2559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one editing CRISPR-Cas construct for one-step genome editing 
            of Streptomyces using AsCas12j-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Tan LL, Heng E, Zulkarnain N, Leong CY, Ng V, Yang LK, San Seow DC, 
            Peh G, Lim YH, Lokanand K, Kanagasundaram YH, Ng SB, Wong FT
  TITLE     Application of Cas12j for Streptomyces editing and cluster 
            activation
  JOURNAL   bioRxiv 2021.10.28.465406
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.28.465406"
COMMENT     Sequence Label: pCRISPomyces-AsCas12j-2
FEATURES             Location/Qualifiers
     source          1..2559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..302
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
ORIGIN
        1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac
       61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg
      121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt
      181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag
      241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg
      301 taatgcccaa gcccgcggtg gagagcgagt tcagcaaggt cctcaagaag cacttccccg
      361 gcgagcggtt ccggtcgtcc tacatgaagc gtggcggcaa gatcctggcg gcccagggcg
      421 aggaagccgt ggtggcgtac ctgcagggca agtcggagga agagccgccg aacttccagc
      481 cgccggccaa gtgccacgtg gtgaccaagt cccgcgactt cgcggagtgg ccgatcatga
      541 aggcctccga ggcgatccag cgctacatct acgccctgtc caccaccgag cgcgcggcct
      601 gcaagccggg caagtcgtcc gagtcccacg cggcctggtt cgcggccacc ggcgtctcca
      661 accacggcta ctcgcacgtg cagggcctga acctgatctt cgaccacacc ctgggccgct
      721 acgacggcgt cctgaagaag gtgcagctgc gcaacgagaa ggcccgcgcc cgcctggagt
      781 ccatcaacgc ctcgcgcgcc gacgagggcc tgccggagat caaggccgag gaagaggaag
      841 tcgcgaccaa cgagacgggc cacctgctgc agccgccggg catcaacccg tccttctacg
      901 tgtaccagac catctcgccg caggcctacc gcccgcgcga cgagatcgtg ctgccgccgg
      961 agtacgcggg ctacgtgcgc gacccgaacg ccccgatccc gctgggcgtc gtgcgcaacc
     1021 gctgcgacat ccagaagggc tgcccgggct acatcccgga gtggcagcgc gaggcgggca
     1081 ccgccatctc ccccaagacc ggcaaggccg tcaccgtgcc gggcctgtcg ccgaagaaga
     1141 acaagcgcat gcgccgctac tggcgctccg agaaggagaa ggcccaggac gccctgctgg
     1201 tcaccgtgcg catcggcacc gactgggtgg tgatcgacgt gcgcggcctg ctgcgcaacg
     1261 cccgctggcg caccatcgcg ccgaaggaca tctcgctgaa cgccctgctg gacctgttca
     1321 ccggcgaccc ggtcatcgac gtgcgtcgca acatcgtgac cttcacctac accctggacg
     1381 cctgcggcac ctacgcccgc aagtggaccc tgaagggcaa gcagaccaag gccaccctgg
     1441 acaagctgac cgccacccag accgtggccc tggtggcgat cgacctgggc cagaccaacc
     1501 cgatctcggc gggcatctcg cgcgtcaccc aggagaacgg cgccctgcag tgcgagccgc
     1561 tggaccgctt caccctgccg gacgacctgc tgaaggacat ctccgcctac cgcatcgcct
     1621 gggaccgcaa cgaggaagag ctgcgcgccc gctccgtcga ggcgctgccg gaggcccagc
     1681 aggccgaggt ccgcgccctg gacggcgtgt cgaaggagac ggcccgcacc cagctgtgcg
     1741 ccgacttcgg cctggacccg aagcgcctgc cgtgggacaa gatgtcgtcc aacaccacct
     1801 tcatctccga ggccctgctg tcgaactccg tctcgcgcga ccaggtgttc ttcaccccgg
     1861 ccccgaagaa gggcgccaag aagaaggccc cggtcgaggt gatgcgcaag gaccgcacct
     1921 gggcccgcgc ctacaagccg cgcctgtccg tcgaggccca gaagctgaag aacgaggccc
     1981 tgtgggccct gaagcgcacc tcgccggagt acctgaagct gtcgcgccgc aaggaagagc
     2041 tgtgccgccg ctcgatcaac tacgtcatcg aaaagacccg tcgccgcacc cagtgccaga
     2101 tcgtcatccc ggtgatcgag gacctgaacg tgcgcttctt ccacggctcg ggcaagcgcc
     2161 tgccgggctg ggacaacttc ttcaccgcca agaaggagaa ccgctggttc atccagggcc
     2221 tgcacaaggc gttctcggac ctgcgcaccc accgctcgtt ctacgtgttc gaggtgcgcc
     2281 cggagcgcac ctcgatcacc tgcccgaagt gcggccactg cgaggtgggc aaccgcgacg
     2341 gcgaggcctt ccagtgcctg tcctgcggca agacctgcaa cgcggacctg gacgtggcca
     2401 cccacaacct gacccaggtg gcgctgaccg gcaagaccat gccgaagcgc gaggagccgc
     2461 gcgacgccca gggcaccgcc ccggcgcgca agaccaagaa ggcgtccaag tcgaaggccc
     2521 cgcccgccga gcgcgaggac cagaccccgg cccaggagc
//
