LOCUS       sequence-37151-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_SOX2
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     137..156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        137..155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccggactct agaggatcga acccttttgg
      121 accctcgtac agaagctaat acgactcact atagggaaat aagagagaaa agaagagtaa
      181 gaagaaatat aagagccacc atgtacaaca tgatggagac ggagctgaag ccgccgggcc
      241 cgcagcaaac ttcggggggc ggcggcggca actccaccgc ggcggcggcc ggcggcaacc
      301 agaaaaacag cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg
      361 ggcagcggcg caagatggcc caagagaacc ccaagatgca caactcggag atcagcaagc
      421 gcctgggcgc cgagtggaaa cttttgtcgg agacggagaa gcggccgttc atcgacgagg
      481 ctaagcggct gcgagcgctg cacatgaagg agcacccgga ttataaatac cggccccggc
      541 ggaaaaccaa gacgctcatg aagaaggata agtacacgct gcccggcggg ctgctggccc
      601 ccggcggcaa tagcatggcg agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga
      661 accagcgcat ggacagttac gcgcacatga acggctggag caacggcagc tacagcatga
      721 tgcaggacca gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga
      781 tgcagcccat gcaccgctac gacgtgagcg ccctgcagta caactccatg accagctcgc
      841 agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcaccc
//
