LOCUS       sequence-371544-        8595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs1 from Salmonella enterica NCTC10718.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: SeAvs1
FEATURES             Location/Qualifiers
     source          1..8595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6493..6540
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6760..7419)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7334..7353
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7420..7522)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7914..7931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8048..8593)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8128..8147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
       61 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc
      121 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
      181 ggtgcctaat gagtgagcta acttacatta attgcgttgc gcattgtgcg tagcacttct
      241 ccagtttttg ttgaaacaga taaagagact aaatcgatca ttcgaaccca aaaatggccg
      301 atttgatgca gacaacgatt taagccatat ctggtagcgc aatcgtcacc tatgacaaaa
      361 gttacatact tgtaatattc tgaattcaat attcttcgtg aaattcattc aatgcttctt
      421 tgagtagtgt tttggcgtta tgataatttc ctaaatatca taaggttatc aggcggtgat
      481 gtatgaggcg atttgtctat ggcgattaaa aacagcgcaa tcatttatgc aggctatgat
      541 tatcagacac tccaaggtgt caggctactg gcggattggc tcaatacacc aactaaatat
      601 aaccgaatag catttgaggc tgatgcgaaa caagttgatg ctccacaagg cattgatgat
      661 attgtctgcg aacgtcagga tggtaaaaca gatttttggc aagttaagtt tacgccagat
      721 accgacaaag aagacaatca actatcatgg gaatggttac tgaaacgtag tggtcatagt
      781 attcgagctc gttctatact gcaaaaaata gctgatgctg ttgataaagt acctgcggaa
      841 agaaggggag atattactct tttgaccaat aaaataccta atcgtgagat agcaacttgc
      901 ttgcgaaata acaaaataga ttggaatcag gttccaattg ctaagcagca aagcattatt
      961 cttcagttag gtacccagga aagagcaaag caatttttcg atatattaca aatatgtcat
     1021 agtgatcaaa gttatacgcg attaaatagt attgtcccag aactacttcg caaacatacc
     1081 aacgaggagg gggtatatcg cctgattgaa cgagctaaac gttgggctat ccagcgtaat
     1141 tcaccttcgg atggtggatg gatatgtctt gaacatattc gtgcagtgat ttcaactaat
     1201 agacctgaac ctattccgca gacttttgtc ttgccagata actatattgt tcctgatgca
     1261 gattttcacg acaaattcat tgattcactt tttaatccta ctaatcgatt agttgtctta
     1321 actggtgctc caggaaaggg taaaagtact tacatcagcc atatttgtca gatattacaa
     1381 actcgcgagt ttccttatat tcgccatcat tattttcttg ggttagatga tcgtacgaca
     1441 gatagattaa gtcccagaat cgttgctgaa gacttgatgt gtcaggtcaa agcattttgc
     1501 tcacaaatcg aaatgaaaaa ttatcatgca gagcacctac ataaagtgct ggctgaatgt
     1561 gggcagatat ataaagaaga aggtaaacga tttttcatca ttattgatgg tttggatcat
     1621 gtctggcgtg ataacggcaa agataaatct ccactggatg agctattttg ccaattgtta
     1681 ccgttgcctg ataatgtaac attattggtt ggtactcaac cagtagatga tgagctattg
     1741 ccatcaagat tgttacagaa cagtccaaga gaagaatggt tgcacctacc aaatatgtca
     1801 ggcgatgcta ttcgtaaata tctatcggga caagttgaaa gtggccgtat cgtattcaat
     1861 tttcatcaaa gccagtatga agaagtttta tcacagtgtg ctgagttgtt gactactaaa
     1921 actcagggat atcctcttca tgttatctac tcatgtgaaa aattacatgt tgaaggtaaa
     1981 gggttatcgc actgggaaat agaaaacctg cctcgctgcg aaggcggaaa cattacaaat
     2041 tattataatg aattatggaa aatattaaat tacgagcaac gcgatattct tcatctctgt
     2101 tgtgcttttc cttttttatg gcctgccaca tcattttctg agattttttc tgagaggact
     2161 gaaactatac cgaatgttaa ggctgtaatc catttgcttt atgagtccat tgctggatta
     2221 agaccgtttc atgaaagctt gattgttttt acccgtagca caactgaaca tgagaataga
     2281 ataaaattat tattgccagc gctaatttca tggctggaga aaagcgcacc caaaccgata
     2341 aaaaattgtt ggtactggtc atgtcttgct tacaatggtg atccatatcc tttaagaaat
     2401 ggcttaacta gagactggat attggaacgg ttggctgaag ggtatcgaca ggatgagttt
     2461 attcgattac tcactcaggc tgaaacttct gctttagccg aagggcattt tagtgaggcc
     2521 tatcagcatc gttcacgcaa gactcgacta cttaatgcta ggttgcaaat ctgggatatg
     2581 tcgacgttgg gcgtttgcag tatgattaat gcttctgaag cattgcttaa acaatatcaa
     2641 tctacccaga atgtcagttc accaaagata ctggcaactt tggctatcgc tttatggttt
     2701 cgtaatcatt tcgatgaagc aaagcgcatt acaagattgg cgttacaacg ctactcaaat
     2761 gaatcatccg tatataccaa taaaaatagc gatgagtcgc gtgctgacat tcgtttatta
     2821 atcaaagctg ctgttttgac tgagtgtttc gatgaaaaat ggttggcaac cggttcagta
     2881 cacaagtgga gtgatagtaa tattaatctg cttatcgaat gtgcggaata taaatcagat
     2941 ataggattac tattttcatt acatgatgtt tttaagcaaa ctgtcataaa aaataaaata
     3001 gtaaatgcga ttgtcagagt tgggattgtt gaacaaatag atttagaata ctggccacat
     3061 ttttctggtc ttgactccgc tctgctgcgg ttatacagtc atttatccac tgcacatcca
     3121 tgttcactta taacagagca aggtgaaagt gaaatcggta gatatcatgt tcatccagaa
     3181 gtatcctacg atgaatggtt ctatgacagc cttttttatc gtcttaatgc cagtggagat
     3241 tattgttggc taccggttag cacgggggaa ggacaggagg aagtcagcag tcattttctc
     3301 catttaaatg atttctcaga tattattgct gaaagtatgg ctctaaatat tcaacaaagc
     3361 ttcagcgatt tttgttcact tattgctttg gtatcagatc ttaaagatca tcaaatgcaa
     3421 atccaacaga agcgaatgtt ttttaaaact gattgggtaa gcattgcttt aaatttacac
     3481 ttaatcatgc attgcaagcc ggttaatacg gaagaaattg atattattct taattctgag
     3541 catacagccc tgtatcggct gcataaaact attcttaact ttcatagtag agccttcgaa
     3601 tctgatgcaa tagcaaactt tctggtattt gaggatggga ggcagaagga aaaactacaa
     3661 gagacaaatg aatatttggc gaataatctt gagttgtcag agattgcgct tcattatgat
     3721 ctcaatcaat caattttttt tgagcgagtc aagttatgtt gggactatgg tctgggatac
     3781 ggacatcata aagatatagc tctgaatcag gtgctgactg caataaaaac tattgcaact
     3841 gttgagccta aatatgcatt aacgcagctt gagcgtgtga gtccattggt tcataatatt
     3901 tgtgacttca cagatggtga ccatactcaa cattccgtaa cggaattgtc tgcgctatat
     3961 gctcatcttt ctccccttac tttaagtagt atctatgaca gttatgttag cgagggtgag
     4021 tggtatgatg cggataatgc attaacgcaa tacttaaaac atgctgatct atcatcacct
     4081 ttcgttgaga gtttatgccg gacattacta gatgatgggc aaattgaaat aatacagaat
     4141 cgtgctaaag acaatgccat attgactacg ttttggccgg aaatattacc acgaaaaatg
     4201 gattatagta gtagcgcaaa acgttcatta agggggactg aaaaatttga tccagcaaaa
     4261 atcagccctg ctgatgtaac taatttactc aatgttcggt caagttatga aaatattcct
     4321 aagtggtatc attattggaa agaccaagga aaagttacag aagtaattaa cgtattgctg
     4381 ccaatcatta ataatggctt gccagaatat agtgaatttc gttatatatt atctgattta
     4441 tttgaagata cattgcgttt gaaaggtaaa aaatatgctt ttcccatttt agtgcaggaa
     4501 catattcagc gaaatggttg gggtgaatgg ggggagtctg atgatcaaac atatgctcgg
     4561 ttagataaag ttatcagatt gtatccggat aaaattgatg actttcttta caagacgact
     4621 cgacttcatc actataaaac taaagaagag aacttggtaa ttcccgggaa taagctaaca
     4681 tatttattag taaatgtagg ccgagtggat gaggcgaaaa gtctatgtga agcgatgatt
     4741 tcggaggtag aggcagaaac ccagaatctt ccgttgtgca aacctcaatg gcaatgggag
     4801 ggagaattag ataacgatat gatcgccgtt aaattcatca ttcgtcgtct tttttggcct
     4861 gttcaatgtg taaaacatct tgtcgctgat caattgtctc atctcttagt taatggtcaa
     4921 tgtgctgaag aaattgaaaa tttacttgta gttgagatgg gaaatcgtca actggagtca
     4981 gaggtggtag atattttaac tgttctctgg ttagctagtt tgaaaggtta taaggttcag
     5041 aataatatat cttcctttat ttatgctcgt agctttcttt cagatgcatt gctggaggct
     5101 atcgttccaa atttaccaaa cctcagtcgc tatcaagtgc tgtataaaca tcctgatgat
     5161 gatggtaatc actatggctt tgaaaaaaca cttggcaatg aacttcccca tatattttgg
     5221 gatgaagtaa aaaggcttga ggagaaatct ggagctccgg ctaaaatatt aatgaaaaaa
     5281 gaatggaatg atatttgtta taatcatgtt caacgatggg aaagggttga ttatttcttc
     5341 ggttcagagc gtgatggttt tactatgagt ttttccacaa ggaatacacg atttggtata
     5401 tctgcatact tgagaaccat taaccggctt atcaacgaat ttagaatgcc aaagcattat
     5461 gcagaacatt attcgatttg tttaatgtca gccaacccat tattttattc cgtatctaat
     5521 caccgacctg gttggttacc tttatggcaa tatggggaga ttaccacaaa ggaaaatgta
     5581 aaaacatatg ttgaggaatg cctgaatgca ttcaaaaatg aacaggaaaa ttcaatatta
     5641 ggagcattgt cattacctgt acgcatcgat gaaaataatt ggttagatat tacggctgtt
     5701 atggggatac aaacagaaga atatgcctct tttaagatac aacatgccga ctgtggtcat
     5761 agtgtagata gtttacttca agcttataga aatattaaat tttcatttgc aaaatgggct
     5821 gaataccaaa attgtgtacc actattggga agtacacgcg aattactgag aatagcacgg
     5881 tgggatataa tgtacgaatt tcgtgggctt ttctcattcg gttgccagga acaggttact
     5941 gcctacccgg ctaaaaatcg tattaacttc gattatcagg gtaaaaccat cggctatagt
     6001 gacttctggc aagcaatacc attatcaatt tatcctaagg atatacgctc acctgttgct
     6061 acttacactg cttatgataa ggaccttgcc tgtaactgga aaaatcatag cgtactgaaa
     6121 aagcctaata tcatgttatg tgattgtaag gtactaaaga gagaaaatag ttacagtcct
     6181 tttgaaatat cagatattcg ttttcacttt gaatctgagc cgttatagta aggattattt
     6241 tgcgataatt aatcaacggg gagctggtca aagtgcctgc tcccatattg actaatatac
     6301 aaatgtgttt gttaagacct ttccaaaggt agggggaatt atgaatttcc gctcctcgct
     6361 catagccgcc tgccagattt aaccccaccc taccacaggg ccccctcaag ccaagccgcc
     6421 gccaatacaa ttttccccca caccaaaacg cctccctccc tagagcacgt actcacaacg
     6481 ccgacgcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     6541 ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     6601 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     6661 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     6721 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     6781 atcgcaatac tgttgtaatt catttaacat tctgccgaca tggaagccat cacagacggc
     6841 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     6901 catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     6961 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     7021 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     7081 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     7141 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca tcgccatacg
     7201 gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     7261 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     7321 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     7381 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     7441 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     7501 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     7561 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     7621 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     7681 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     7741 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     7801 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     7861 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     7921 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     7981 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     8041 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     8101 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     8161 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     8221 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     8281 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     8341 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     8401 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     8461 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     8521 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     8581 aaaacgatct caaga
//
