LOCUS       sequence-371547-        4907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage T5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_T5
FEATURES             Location/Qualifiers
     source          1..4907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(409..1287)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1314..1598
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1521..1540
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2969..2996
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3015..3106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3107..3175,3176..3967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3325..3344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4138..4726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4627..4646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4880..4897
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg
      301 cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
      361 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
      421 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
      481 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
      541 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
      601 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
      661 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
      721 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
      781 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
      841 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
      901 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
      961 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
     1021 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
     1081 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
     1141 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
     1201 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
     1261 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac
     1321 caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct
     1381 aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat
     1441 gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt
     1501 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
     1561 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg
     1621 aggaattaac catggaagta tcaagaccct acgttaatac agtagatgtt atcgatttcg
     1681 ggatagataa acgtttcttt cgtctacctg tttcaggaat actagcacaa gaaggtatta
     1741 cacctaatgg tcctcaaata gcgattatta atgccctaga ggaccctaga catcgttttg
     1801 taacagcgtg tgtgtcacgt cgtgtaggca aatccttcat cgcatacaca cttgggttcc
     1861 taaaattgct ggaacctaat gtgaaggtac tagtagttgc ccctaactac tcactggcta
     1921 acattggttg gtcacagatt cgtggtctta tcaagaagta tggcctacaa accgaacgtg
     1981 aaaacgccaa agataaagag attgagctgg caaacggctc gctcttcaaa ctagcttctg
     2041 cggcgcaggc tgactccgca gttggacgtt catatgattt tatcatcttt gacgaagcgg
     2101 caatttccga tgtgggtggt gatgctttca gggttcagct gcgtcctact ctggataaac
     2161 ctaattctaa ggctctattt atttccaccc ctcgtggagg taactggttt aaagagtttt
     2221 acgcctacgg atttgatgat acgttgccta actgggtatc tattcatggt acatatcgtg
     2281 ataacccacg tgctgacttg aatgatattg aggaagcacg ccgtacagtt agtaaaaact
     2341 acttccgcca agaatacgag gctgacttct ctgtattcga gggccagatc tttgatacct
     2401 ttaatgctat cgatcatgtt aaagacctca aaggtatgcg tcacttcttt aaagatgatg
     2461 aagcattcga aacgttgctt ggtattgacg ttggttatcg tgatcctaca gcagttctta
     2521 ctattaaata tcattacgat acggatactt actatgtatt agaagagtac cagcaggcgg
     2581 agaaaactac agctcagcat gctgcttata ttcagcactg tatagatcgt tataaagttg
     2641 atcgtatttt tgttgactct gcagcagctc agttccgcca ggatttagca tatgagcatg
     2701 aaattgcatc tgctccagct aaaaagtctg tcctagatgg tctagcatgc ctgcaagcgc
     2761 tattccagca gggcaagatt attgtagatg cttcatgttc ctccttaatt catgcattgc
     2821 agaactataa gtgggacttc caagaaggcg aagagaaatt atcacgtgaa aaaccacgtc
     2881 atgatgctaa ctctcacctt tgtgatgcgc tccgctatgg aatttactct atttcccgtg
     2941 gtaaataatg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     3001 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtr ttgacgccgg
     3361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcratggc
     3661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3781 tggctggttt attgctgata aatcyggagc cggtgagcgt gggtctcgcg gtatcattgc
     3841 agcrctgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4261 cagagcgcag ataccaaata ctgtycttct agtgtagccg tagttagscc accacttcaa
     4321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
