LOCUS       sequence-371572-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: 
            PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..65
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             753..800
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             804..893
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
       61 gaccgatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      121 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      181 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      241 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
      301 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
      361 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      421 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
      481 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
      541 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
      601 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
      661 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
      721 ccagatactg cgacctccct agcaaactgg ggaagcgacc tgccgccaca aagaaggctg
      781 gacaggctaa gaagaagaaa ggctacccat acgatgttcc agattacgct ggctatccct
      841 atgacgtccc ggactatgca ggttcatatc catatgacgt tccagattac gcttgataa
//
