LOCUS       sequence-37158-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_KLF4
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     138..157
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        138..156
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcg aacccttttg
      121 gaccctcgta cagaagctaa tacgactcac tatagggaaa taagagagaa aagaagagta
      181 agaagaaata taagagccac catggctgtc agcgacgcgc tgctcccatc tttctccacg
      241 ttcgcgtctg gcccggcggg aagggagaag acactgcgtc aagcaggtgc cccgaataac
      301 cgctggcggg aggagctctc ccacatgaag cgacttcccc cagtgcttcc cggccgcccc
      361 tatgacctgg cggcggcgac cgtggccaca gacctggaga gcggcggagc cggtgcggct
      421 tgcggcggta gcaacctggc gcccctacct cggagagaga ccgaggagtt caacgatctc
      481 ctggacctgg actttattct ctccaattcg ctgacccatc ctccggagtc agtggccgcc
      541 accgtgtcct cgtcagcgtc agcctcctct tcgtcgtcgc cgtcgagcag cggccctgcc
      601 agcgcgccct ccacctgcag cttcacctat ccgatccggg ccgggaacga cccgggcgtg
      661 gcgccgggcg gcacgggcgg aggcctcctc tatggcaggg agtccgctcc ccctccgacg
      721 gctcccttca acctggcgga catcaacgac gtgagcccct cgggcggctt cgtggccgag
      781 ctcctgcggc cagaattgga cccggtgtac attccgccgc agcagccgca gccgccaggt
      841 ggcgggct
//
