LOCUS       sequence-37159-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc
            JA, Weinstein BM, Lawson ND
  TITLE     A genetic screen for vascular mutants in zebrafish reveals dynamic 
            roles for Vegf/Plcg1 signaling during artery development.
  JOURNAL   Dev Biol. 2009 May 15. 329(2):212-26.
  PUBMED    19269286
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2009 May 15. 329(2):212-26."
COMMENT     Sequence Label: 786 pCSmAKT2Acherry
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..164)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    175..199
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
       61 aactccttga tgatggccat gttgtcctcc tcgcccttgc tcaccatagg accggggttt
      121 tcttccacgt ctcctgcttg ctttaacaga gagaagttcg tggctccgga tccccccact
      181 ttgtacaaga aagctgggta ggctgtgcca ctggctgagt agaagaactg ggggaagtgc
      241 ggcctccgct cactgtccac acactccatg ctgtcatctt gatcaggcgg cgtgatggtg
      301 atcatctgag ctgtgaactc ctcatcgaaa tacctggtgt cagtctcaga ggtgacctgg
      361 ggcttgaaag gtgggctcag cttcttctca tacacatcct gccacacgat gttggcaaag
      421 aaccggtgct gcatgatctc cttggcatcc tcagagcccc caccgagcct ctgtgtaggg
      481 tccttcttga gcagcccgga gagcagggac ttggcctcag ggccgagtgt gcgcgggaag
      541 cggatctcct ccatgaggat cagctcgaac agcttctcgt ggtcctggtt gtagaagggc
      601 aggcggccac acatcatctc atacatgacc acgcccagcc cccaccagtc cactgcacgg
      661 ccgtagtcgt tgtcctccag cacctcaggg gccaggtact ccggcgttcc gcagaatgtc
      721 ttcatagtgg caccatcctt gatcccctcc ttgcacagcc cgaagtccgt tatcttgatg
      781 tgcccgtcct tgtccagcat gaggttctcc agcttcaggt cccggtacac cacgttcttc
      841 tcggagtgca agtagtccag ggcagacaca atctccgcac
//
