LOCUS       sequence-371626-       14658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of Rbfox2-rABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Lin Y, Kwok S, Hein AE, Thai BQ, Alabi Y, Ostrowski MS, Wu K, Floor 
            SN
  TITLE     RNA molecular recording with an engineered RNA deaminase.
  JOURNAL   Nat Methods. 2023 Dec;20(12):1887-1899. doi: 
            10.1038/s41592-023-02046-z. Epub 2023 Oct 19.
  PUBMED    37857907
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02046-z"; journalName: "Nat Methods"; date: 
            "2023-12"; volume: "20"; issue: "12"; pages: "1887-1899"
COMMENT     Sequence Label: pSLIK-Rbfox2-rABE-Flag
FEATURES             Location/Qualifiers
     source          1..14658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1376..1964
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1429..1449)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1847..1858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1967..1983)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1968..1984)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2493..2673
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2699..2716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2705..2929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2975..3403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3062..3081)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3272..3293
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3412..3432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3417..3746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3597..3732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3659..3678
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3794..3841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3860..4234
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4364..4485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4401..4420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4455..4474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4534..4550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4534..4550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4547..4569)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4558..4574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4582..4588,4589..4606,4607..4612))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4627..4648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4765..4782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4936..5524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5016..5035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5695..6486,6487..6555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6318..6337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6556..6660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6735..6754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6926..7305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7307..7505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7523..7703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7750..7875
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8368..8601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8786..8830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9128..9245
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9302..9326
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9358..9672
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9366..9384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9402..9420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9437..9455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9473..9491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9509..9527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9544..9562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9580..9598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9645..9669
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11667..11690
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(11708..11732)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        11782..12993
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     12830..12849
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(13118..13735,13736..13741,13742..13777,13778..13780,
                     13781..13816,13817..13819,13820..13855,13856..13864)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    13917..14490
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(14084..14101)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     14311..14330
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(14549..14658,1..910)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             join(14549..14658,1..910)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
ORIGIN
        1 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
       61 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
      121 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
      181 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
      241 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
      301 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
      361 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
      421 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
      481 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
      541 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
      601 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
      661 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
      721 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
      781 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
      841 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
      901 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
      961 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
     1021 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
     1081 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
     1141 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
     1201 tctagatctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     1261 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1321 atgaatgcaa ttgttgttgt ttagtccctc ccaattcgat atcaagctta tcgataatca
     1381 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1441 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1501 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1561 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1621 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1681 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1741 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     1801 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1861 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     1921 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     1981 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     2041 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     2101 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     2161 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     2221 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     2281 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     2341 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     2401 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     2461 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     2521 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2581 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2641 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     2701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     3421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     3721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     3781 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     3841 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     3901 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     3961 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     4021 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     4081 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     4141 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     4201 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     4261 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4321 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     4381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4441 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     4501 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4561 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4621 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4681 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6661 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     6721 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     6781 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     6841 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     6901 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     6961 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     7021 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     7081 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     7141 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     7201 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     7261 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     7321 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     7381 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     7441 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     7501 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7681 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7741 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7801 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7861 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7921 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7981 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8041 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8101 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8161 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8221 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8281 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8341 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8401 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8461 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8521 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8581 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8641 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8701 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8761 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8821 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8881 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8941 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9001 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9061 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     9121 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9181 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9241 attttcgggt ttattacagg gacagcagag atccagtttg gttaattcta gacagttatc
     9301 aacaagtttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatcccgag
     9361 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     9421 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     9481 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     9541 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     9601 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     9661 cgtcagatcg ccactagtcc accatgttaa ttaaagcgga gggcgcccag ccgcagcagc
     9721 cgcctcagct cgggcccggc gccgccgccc ggggcatgaa gcgggagtcg gagctggagc
     9781 tgccggtgcc cggagcggga ggagacggag ccgatcccgg cctgagcaag cggccgcgca
     9841 ctgaggaggc ggcggccgac ggtggcggcg ggatgcagaa cgagcctctg actccaggtt
     9901 atcatggatt cccagcgcgg gacagccagg gtaaccagga gccgacaaca actcctgacg
     9961 caatggttca gccttttact accatcccat ttccaccacc tccgcagaat ggaattccca
    10021 cagagtatgg ggtgccacac actcaagact atgccggcca gaccggtgag cataacctga
    10081 cactctacgg aagtacgcaa gcccacgggg agcagagcag caactcaccc agcacacaaa
    10141 atggatctct tacgcagaca gaaggtggag cacagacaga cggccagcag tcacagacac
    10201 aaagtagtga aaattcagag agtaaatcta ccccgaaacg gctgcatgtc tctaatattc
    10261 ctttccgctt ccgggaccct gacctccggc agatgtttgg gcagtttggc aaaatcctag
    10321 atgtagaaat aatctttaat gaacgtggct ctaagggatt cgggttcgta actttcgaga
    10381 atagtgctga tgcagacagg gccagggaga aattacacgg caccgtggta gagggccgta
    10441 aaatcgaggt gaataatgct acagcacgtg taatgaccaa taagaagatg gtcacaccat
    10501 atgcaaatgg ttggaaatta agcccagtag ttggagctgt atatggtccg gagttatatg
    10561 cagcatccag ctttcaagca gatgtgtccc taggcaatga tgcagcagtg cccctatcag
    10621 gaagaggggg tatcaacact tacattcctt taatcagtct ccctttagtt cctggcttcc
    10681 cttaccctac tgcagccacc acggcagccg ctttcagagg agcccatttg aggggcagag
    10741 ggcggacagt atatggtgca gtccgagcgg tacctccaac agccatcccc gcctatccag
    10801 gtgtggttta ccaggacgga ttttacggtg ctgacctcta tggtggatat gcagcctaca
    10861 gatatgcaca gcctgctact gcaaccgcag ccaccgctgc tgcagccgct gcagccgctt
    10921 acagtgacgg ttatggcagg gtgtacacag ccgaccccta ccatgccctt gcccctgccg
    10981 ctagctatgg agttggcgct gtggcgagtt tataccgagg tggctacagc cgatttgccc
    11041 cctactcagg tgggtccagt gggggctcca gcgggtcaga aactcctggg acgagtgaat
    11101 ctgcgacccc cgagtcatcc gggggtagct ccggtggttc catgtctgag gtggaattct
    11161 cgcacgaata ctggatgaga cacgcattga ctctcgcaaa gagggctcgg gatgaacgcg
    11221 aggtgcccgt gggggcagta ctcgtgctta acaatcgcgt aatcggcgaa ggttggaata
    11281 gggcgatcgg actccacgac cccactgcac atgcggaaat catggccctt cgacagggag
    11341 ggcttgtgat gcagaattat cgacttatcg atgcgacgct gtacgtcacg tttgaacctt
    11401 gcgtaatgtg cgcgggagct atgattcact cccgcattgg acgagttgta ttcggtgtcc
    11461 gcaacgccaa gacgggtgcc gcaggttcac tgatggacgt gctgcattac ccaggcatga
    11521 accaccgggt agaaatcaca gaaggcatat tggcggacga atgtgcggcg ctgttgagct
    11581 acttttttcg catgccgagg caggtattta atgcgcaaaa gaaggctcag tcttcaacag
    11641 atagcggtgg aagcagtgga ggctcagatt acaaggatga cgatgacaag tgactcgaga
    11701 tatctagacc cagctttctt gtacaaagtg gttgataaca tctcgagcca taaagatggt
    11761 taattaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
    11821 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
    11881 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
    11941 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
    12001 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
    12061 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
    12121 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
    12181 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
    12241 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
    12301 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
    12361 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
    12421 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
    12481 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
    12541 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
    12601 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
    12661 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
    12721 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
    12781 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
    12841 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
    12901 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
    12961 aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc
    13021 accccgtacc ggtcctgcag gatttaaatg gcgcgccgct agcgctaccg gactcagatc
    13081 tcgagctcaa gcttcgaatt ctgcagtcga attcaccatg tctagactgg acaagagcaa
    13141 agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag
    13201 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
    13261 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
    13321 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg
    13381 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
    13441 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
    13501 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
    13561 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
    13621 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
    13681 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
    13741 gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
    13801 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
    13861 gtaactaagt aaggatccgc ggccgcacta gaggaattcc gcccctctcc cccccccccc
    13921 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
    13981 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
    14041 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
    14101 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
    14161 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
    14221 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
    14281 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
    14341 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
    14401 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
    14461 gtggttttcc tttgaaaaac acgatgataa gcttaccggt acgcgtgata gcttgccaca
    14521 acccgtacca aagatggata gatccggaaa gcctgaactc accgcgacgt ctgtcgagaa
    14581 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    14641 atctcgtgct ttcagctt
//
