LOCUS       sequence-371633-       12134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac compatible plasmid expressing dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12134)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 12134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: NAX_CAGGS_CAS9_m4_mcherry
FEATURES             Location/Qualifiers
     source          1..12134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          199..1215
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1139..1161
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1223..1242
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1312..1321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1318..1344,1345..1347,1348..3831,3832..3837,3838..3903,
                     3904..3906,3907..5421)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5434..5454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5479..5498)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5523..5571
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5803..6014
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6024..6033
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6148..6166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6416..6435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6717..6838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6754..6773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6808..6827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7601..7635
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8126..8142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8126..8142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8139..8161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8150..8166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8174..8180,8181..8198,8199..8204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8219..8240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8357..8374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8528..9116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8608..8627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9287..10078,10079..10147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9910..9929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10148..10252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10278..10733)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10415..10436)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10627..10646
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10860..10882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10874..10891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10875..10891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   11021..11083
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11504..11883
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 awtnkdkkkk kggggggggg gggggggggg gggggggggg ngcgcgcgcc rggcggggsg
       61 gggcggggcg rggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      121 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      181 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      241 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      301 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
      361 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
      421 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
      481 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt ttgcgctccg cagtgtgcgc
      541 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
      601 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
      661 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
      721 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
      781 ggggtgccgg gcggggcggg gccgcctcgg gccggggrgg gctcgggggr ggggcgcggc
      841 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
      901 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
      961 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     1021 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     1081 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1141 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     1201 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     1261 aaagaatcta gataccctcg taaaggtcta gagctagcgg atcgaaccct tgccaccatg
     1321 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt
     1381 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
     1441 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc
     1501 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
     1561 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
     1621 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
     1681 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
     1741 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
     1801 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
     1861 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
     1921 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
     1981 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
     2041 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
     2101 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
     2161 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
     2221 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg
     2281 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     2341 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     2401 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     2461 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     2521 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct
     2581 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     2641 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     2701 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc
     2761 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     2821 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     2881 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     2941 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     3001 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     3061 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     3121 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     3181 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     3241 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
     3301 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     3361 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
     3421 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     3481 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     3541 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     3601 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
     3661 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     3721 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     3781 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggctgctatc
     3841 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     3901 aaagctagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     3961 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     4021 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
     4081 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     4141 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     4201 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     4261 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     4321 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     4381 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     4441 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     4501 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     4561 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     4621 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     4681 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     4741 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     4801 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     4861 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     4921 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag
     4981 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     5041 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     5101 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     5161 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     5221 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     5281 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
     5341 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     5401 ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgtgagtt
     5461 taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5521 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5581 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5641 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5701 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgaaat cggatccgcg
     5761 gccgcactga ttctagctgc agaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     5821 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     5881 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     5941 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     6001 ccgccagaac acaggatcct cgagccacca tggccatcat caaggagttc atgcgcttca
     6061 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6181 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     6241 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     6301 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     6361 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6421 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     6481 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     6541 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     6601 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6661 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagttaact
     6721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6841 atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct
     6901 tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct
     6961 tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc
     7021 cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg
     7081 cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     7141 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     7201 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     7261 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     7321 gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     7381 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     7441 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     7501 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     7561 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     7621 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     7681 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     7741 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     7801 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     7861 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     7921 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     7981 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     8041 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     8101 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10261 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    10321 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    10381 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    10441 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    10501 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10561 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10621 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10681 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
    10741 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10801 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10861 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga
    10921 acccgtggag gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat
    10981 gaagatgctc gacacgctgc agaacacgca gctagattaa ccctagaaag ataatcatat
    11041 tgtgacgtac gttaaagata atcatgtgta aaattgacgc atgtgtttta tcggtctgta
    11101 tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt acacttacat
    11161 actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaacaaa
    11221 aactcaaaat ttcttctata aagtaacaaa acttttatga gggacagccc ccccccaaag
    11281 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
    11341 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
    11401 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
    11461 ctgcagacac ctggggggat acggggaaaa ggcctccacg gccgacattg attattgact
    11521 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    11581 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    11641 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    11701 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    11761 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    11821 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    11881 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
    11941 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
    12001 ggggggsgsg ssccgggggg gngggggggg gggggggggg gggggggggg gggggggggg
    12061 gggggggggg ggggbsngnn nngngggggg ggggssgsgg ggggkgkgkk knnkgggggg
    12121 gggggggggg gggg
//
