LOCUS       sequence-371635-       12266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac compatible plasmid expressing dCas9-KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: NAX_CAGGS_CAS9_m4_KRAB_mcherry
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          136..1153
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1077..1099
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1161..1180
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1250..1259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1256..1282,1283..1285,1286..3769,3770..3775,3776..3841,
                     3842..3844,3845..5359)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5372..5392
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5453..5647
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5702..5721)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5746..5794
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6026..6237
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6247..6256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6371..6389)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6639..6658
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6940..7061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6977..6996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7031..7050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7824..7858
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8349..8365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8349..8365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8362..8384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8373..8389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8397..8403,8404..8421,8422..8427))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8442..8463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8580..8597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8751..9339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8831..8850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9510..10301,10302..10370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10133..10152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10371..10475)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10501..10956)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10638..10659)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10850..10869
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11083..11105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11097..11114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11098..11114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   11244..11306
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11727..12106
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
       61 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
      121 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
      181 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
      241 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
      301 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
      361 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
      421 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttttg cgctccgcag tgtgcgcgag
      481 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
      541 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
      601 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
      661 skccgtacgg ggcgkggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggkgggg
      721 gtgcsgggcg gggcggggcc gccksgggcs ggggrgggmy sgggggrggg gcgcggcngg
      781 cccccggrgc gccggsggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
      841 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
      901 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
      961 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1021 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1081 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     1141 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     1201 agaatctaga taccctcgta aaggtctaga gctagcggat cgaacccttg ccaccatgga
     1261 caagaagtac tccattgggc tcgctatcgg cacaaacagc gtcggctggg ccgtcattac
     1321 ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag
     1381 cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac
     1441 gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct
     1501 gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga
     1561 ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat
     1621 cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct
     1681 tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat
     1741 caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga
     1801 caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa
     1861 cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct
     1921 cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat
     1981 cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc
     2041 caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat
     2101 cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct
     2161 gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat
     2221 caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca
     2281 actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata
     2341 cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa
     2401 aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca
     2461 gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat
     2521 cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa
     2581 aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt
     2641 cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg aggaagtcgt
     2701 ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct
     2761 gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa
     2821 cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg
     2881 agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa
     2941 acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg
     3001 agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa
     3061 agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac
     3121 ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct
     3181 cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct
     3241 gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt
     3301 tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct
     3361 cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga
     3421 gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa
     3481 ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga
     3541 gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa
     3601 gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga
     3661 aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat
     3721 gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg ctgctatcgt
     3781 gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa
     3841 agctagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta
     3901 ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa
     3961 ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt
     4021 tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa
     4081 gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct
     4141 ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca
     4201 ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc
     4261 caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat
     4321 cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat
     4381 tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact
     4441 tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac
     4501 agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac
     4561 cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg
     4621 caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag
     4681 tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga
     4741 actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct
     4801 cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc
     4861 tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa
     4921 aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta
     4981 tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca
     5041 caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct
     5101 cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat
     5161 cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc
     5221 agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt
     5281 cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct
     5341 ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg tgagtggtgg
     5401 aggaagtggc gggtcagggt cgatggacgc gaaatcactt acggcatggt cgagaacact
     5461 ggttacgttc aaggacgtgt ttgtggactt tacacgtgag gagtggaaat tgctggatac
     5521 tgcgcaacaa attgtgtatc gaaatgtcat gcttgagaat tacaagaacc tcgtcagtct
     5581 cggataccag ttgacgaaac cggatgtgat ccttaggctc gaaaaggggg aagaaccttg
     5641 gctggtatag aagaattcga tccctaccgg ttagtaatga gtttaaacgg gggaggctaa
     5701 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     5761 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5821 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5881 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caatgtcggg
     5941 gcggcaggcc ctgccatagc agatctgcga aatcggatcc gcggccgcac tgattctagc
     6001 tgcagaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6061 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     6121 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6181 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggat
     6241 cctcgagcca ccatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     6301 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     6361 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     6421 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     6481 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     6541 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     6601 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     6661 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     6721 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     6781 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     6841 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     6901 cactccaccg gcggcatgga cgagctgtac aagtaagtta acttgtttat tgcagcttat
     6961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc
     7081 aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat
     7141 ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg
     7201 tcaaccaggt tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc
     7261 agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt
     7321 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     7381 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     7441 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     7501 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa
     7561 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     7621 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     7681 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     7741 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     7801 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     7861 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
     7921 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
     7981 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
     8041 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
     8101 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
     8161 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
     8221 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
     8281 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg
     8341 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8401 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8461 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8521 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8821 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8881 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8941 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9001 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9061 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9361 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10501 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
    10561 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
    10621 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
    10681 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
    10741 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
    10801 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
    10861 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
    10921 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
    10981 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    11041 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    11101 aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc
    11161 agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc
    11221 tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag
    11281 ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
    11341 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
    11401 caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct
    11461 ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag ggatgtaatt
    11521 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
    11581 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
    11641 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
    11701 gatacgggga aaaggcctcc acggccgaca ttgattattg actagttatt aatagtaatc
    11761 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    11821 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    11881 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    11941 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    12001 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    12061 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
    12121 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
    12181 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggg sgsgscrggs
    12241 ggggsggggg gggggggggg gsgggg
//
