LOCUS       sequence-371641-        9761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding a human codon-optimized High-fidelity RfxCas13d 
            (hfCas13d) driven by CBh promoter, guide RNAs compatible with 
            RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Tong H, Huang J, Xiao Q, He B, Dong X, Liu Y, Yang X, Han D, Wang Z,
            Wang X, Ying W, Zhang R, Wei Y, Xu C, Zhou Y, Li Y, Cai M, Wang Q, 
            Xue M, Li G, Fang K, Zhang H, Yang H
  TITLE     High-fidelity Cas13 variants for targeted RNA degradation with 
            minimal collateral effects.
  JOURNAL   Nat Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7.
  PUBMED    35953673
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7."
COMMENT     Sequence Label: 
            pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EG
            FP-pA-pSV40-mCherry-pA
FEATURES             Location/Qualifiers
     source          1..9761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          961..1188
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1209..1229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4146..4172
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4173..4193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4206..4340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4347..4595
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4347..4367
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4518..4537
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4597..4632
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     promoter        4664..4735
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4737..5094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4737..4928
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      4945..5080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5135..5144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5141..5143,5144..5146,5147..5860)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5141..5143,5144..5146,5147..5857)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5186..5207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5447..5466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5794..5815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5983..6104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6111..6182
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6184..6541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6184..6375
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      6392..6527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6582..6591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6588..7298
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    7421..7542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7899..7933
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        7964..8068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8069..8137,8138..8929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8287..8306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9100..9688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9589..9608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggsgcgcs csrggsgggg sggggsgggg sgrggggsgg
      841 ggsggggsgr ggsggrgrgg kgsggsggcr gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgrggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1081 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1141 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1201 ccaccatgcc taaaaagaaa agaaaggtgg gttctggtat cgagaagaag aagagcttcg
     1261 ccaagggcat gggagtgaag agcaccctgg tgtccggctc taaggtgtac atgaccacat
     1321 ttgctgaggg aagcgacgcc aggctggaga agatcgtgga gggcgatagc atcagatccg
     1381 tgaacgaggg agaggctttc agcgccgaga tggctgacaa gaacgctggc tacaagatcg
     1441 gaaacgccaa gttttcccac ccaaagggct acgccgtggt ggctaacaac ccactgtaca
     1501 ccggaccagt gcagcaggac atgctgggac tgaaggagac actggagaag aggtacttcg
     1561 gcgagtccgc cgacggaaac gataacatct gcatccaggt catccacaac atcctggata
     1621 tcgagaagat cctggtggag tacatcacaa acgtggtgta cgtggtgaac aacatctccg
     1681 gcctggacaa ggatatcatc ggcttcggaa agttttctac cgtgtacaca tacgacgagt
     1741 tcaaggatcc agagcaccac cgggccgctt ttaacaacaa cgacaagctg atcaacgcca
     1801 tcaaggctca gtacgacgag ttcgataact ttctggataa ccccaggctg ggctacttcg
     1861 gacaggcttt cttttctaag gagggcagaa actacatcat caactacgga aacgagtgtt
     1921 acgacatcct ggccctgctg agcggactga ggcactgggt ggtgcacaac aacgaggagg
     1981 agtctcggat cagccgcacc tggctgtaca acctggacaa gaacctggat aacgagtaca
     2041 tctccacact gaactacctg tacgacagga tcaccaacga gctgacaaac agcttctcca
     2101 agaactctgc cgctaacgtg aactacatcg ctgagaccct gggcatcaac ccagctgagt
     2161 tcgctgagca gtacttcaga ttttccatca tgaaggagca gaagaacctg ggcttcaaca
     2221 tcacaaagct gagagaagtg atgctggaca gaaaggatat gtccgagatc aggaagaacc
     2281 acaaggtgtt cgattctatc agaaccaagg tgtacacaat gatggacttt gtgatctaca
     2341 ggtactacat cgaggaggat gccaaggtgg ccgctgccaa caagagcctg cccgacaacg
     2401 agaagtctct gagcgagaag gatatcttcg tgatcaacct gagaggctcc tttaacgacg
     2461 atcagaagga cgctctgtac tacgatgagg ccaacaggat ctggagaaag ctggagaaca
     2521 tcatgcacaa catcaaggag ttccggggaa acaagacccg cgagtacaag aagaaggacg
     2581 ctccaaggct gcctaggatc ctgcctgctg gaagggacgt gagcgccttc agcaagctga
     2641 tgtacgccct gacaatgttt ctggacggaa aggagatcaa cgatctgctg accacactga
     2701 tcaacaagtt cgacaacatc cagtcttttc tgaaagtgat gcctctgatc ggcgtgaacg
     2761 ctaagttcgt ggaggagtac gccttcttta aggacagcgc caagatcgct gatgagctgc
     2821 ggctgatcaa gtcctttgcc aggatgggag agccaatcgc tgacgctagg agagctatgt
     2881 acatcgatgc catccggatc ctgggaacca acctgtctta cgacgagctg aaggctctgg
     2941 ccgacacctt cagcctggat gagaacggca acaagctgaa gaagggcaag cacggaatgc
     3001 gcaacttcat catcaacaac gtgatcagca acaagcggtt tcactacctg atcagatacg
     3061 gcgacccagc tcacctgcac gagatcgcta agaacgaggc cgtggtgaag ttcgtgctgg
     3121 gacggatcgc cgatatccag aagaagcagg gccagaacgg aaagaaccag atcgaccgct
     3181 actacgagac ctgcatcggc aaggataagg gaaagtccgt gtctgagaag gtggacgctc
     3241 tgaccaagat catcacaggc atgaactacg accagttcga taagaagaga tctgtgatcg
     3301 aggacaccgg aagggagaac gccgagagag agaagtttaa gaagatcatc agcctgtacc
     3361 tgacagtgat ctaccacatc ctgaagaaca tcgtgaacat caacgctaga tacgtgatcg
     3421 gcttccactg cgtggagcgc gatgcccagc tgtacaagga gaagggatac gacatcaacc
     3481 tgaagaagct ggaggagaag ggctttagct ccgtgaccaa gctgtgcgct ggaatcgacg
     3541 agacagcccc cgacaagagg aaggatgtgg agaaggagat ggccgagaga gctaaggaga
     3601 gcatcgactc cctggagtct gctaacccta agctgtacgc caactacatc aagtactccg
     3661 atgagaagaa ggccgaggag ttcaccaggc agatcaacag agagaaggcc aagaccgctc
     3721 tgaacgccta cctgaggaac acaaagtgga acgtgatcat ccgggaggac ctgctgcgca
     3781 tcgataacaa gacctgtaca ctgttccgga acaaggctgt gcacctggag gtggctcgct
     3841 acgtgcacgc ctacatcaac gacatcgccg aggtgaactc ctactttcag ctgtaccact
     3901 acatcatgca gaggatcatc atgaacgaga gatacgagaa gtctagcggc aaggtgtctg
     3961 agtacttcga cgccgtgaac gatgagaaga agtacaacga tagactgctg aagctgctgt
     4021 gcgtgccttt cggatactgt atcccacggt ttaagaacct gagcatcgag gccctgttcg
     4081 accgcaacga ggctgccaag tttgataagg agaagaagaa ggtgagcggc aactccggtt
     4141 ctggttaccc atacgacgta ccagattacg ctcccaagaa gaagcgcaag gtgggataaa
     4201 agcttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4321 tatcttatca tgtctggatc actagtgagg gcctatttcc catgattcct tcatatttgc
     4381 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     4441 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     4501 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     4561 tggctttata tatcttgtgg aaaggacgaa acaccgcaag taaaccccta ccaactggtc
     4621 ggggtttgaa acgggtcttc gagaagacct tttttttacg cgtcattcaa atatgtatcc
     4681 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     4741 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4801 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4861 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4921 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4981 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5041 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     5101 tcaagagaca ggatgaggat cgtttcgctc tagagccacc atggtgagca agggcgagga
     5161 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5221 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5281 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5341 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5401 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5461 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5521 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5581 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5641 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5701 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5761 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5821 cgccgggatc actctcggca tggacgagct gtacaagtga agcggccgcg actctagatc
     5881 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5941 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     6001 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6061 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttagtcgac cattcaaata
     6121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6181 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6361 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6481 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6541 agatcgatca agagacagga tgaggatcgt ttcgctctag agccaccatg gtgagcaagg
     6601 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     6661 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     6721 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     6781 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     6841 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     6901 atgacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     6961 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaaa
     7021 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     7081 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     7141 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     7201 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     7261 gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     7321 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7381 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tagtcgacgg cgcgccctag
     7561 acgaagcagc tccagcctac acaatcgctc aagacgtgta atgcttttat tatatattag
     7621 tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     7681 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     7741 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     7801 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     7861 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     7921 tatctttcta gggttaagga tccgcacttt tcggggaaat gtgcgcggaa cccctatttg
     7981 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8041 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8101 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8161 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8221 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8281 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8341 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8401 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8461 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8521 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8581 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8641 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8701 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8761 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8821 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8881 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8941 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9001 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9061 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9121 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9181 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9241 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9301 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9361 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9421 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9481 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9541 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9601 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9661 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9721 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg t
//
