LOCUS       sequence-371642-       11191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered 
            PLD mutant with 30 times higher activity than the wild-type).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Tei R, Bagde SR, Fromme JC, Baskin JM
  TITLE     Activity-based directed evolution of a membrane editor in mammalian 
            cells.
  JOURNAL   Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0.
  PUBMED    37217787
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2023 May 22. doi: 10.1038/s41557-023-01214-0."
COMMENT     Sequence Label: pCDH-CRY2-mCh-superPLDx30
FEATURES             Location/Qualifiers
     source          1..11191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      34..169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(202..218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(202..218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(215..237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    226..242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(250..256,257..274,275..280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    295..316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(433..450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(604..1192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(1363..2154,2155..2223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1986..2005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2224..2328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2396..2414
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2452..2474)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2574..2593
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2787..2809
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2801..2818
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2802..2818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2833..3059
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3060..3240
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3287..3412
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3905..4138
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4323..4367
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4634..4750
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4840..5043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4994..5014
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5040..5064
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5115..6608
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             6642..7346
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6784..6802)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7052..7071
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8972..9183
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9196..9464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             9491..10090
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9491..9510)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9987..10007
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10097..10685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10150..10170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10568..10579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10759..10992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11064..11185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11101..11120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11155..11174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
       61 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
      181 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
      241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1021 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2401 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     2461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2581 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     2641 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     2701 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     2761 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     2821 caagctgacg cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     2881 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     2941 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     3001 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     3061 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3121 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3181 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3241 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     3301 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     3361 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     3421 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     3481 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     3541 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3601 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3661 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3721 aaacaaaagt aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag
     3781 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3841 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3901 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3961 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4021 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4081 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4141 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4201 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4261 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4321 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4381 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4441 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4501 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4561 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     4621 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     4681 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
     4741 tcaaaatttt atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact
     4801 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4861 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4921 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4981 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga
     5041 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     5101 agaagattgc caccatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     5161 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     5221 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     5281 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     5341 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     5401 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     5461 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     5521 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     5581 ggaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     5641 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     5701 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     5761 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     5821 gcaagaaagt tgttgggaac tctacttcac tactttctcc gtatctccat ttcggggaaa
     5881 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     5941 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     6001 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     6061 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     6121 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     6181 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     6241 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     6301 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     6361 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     6421 agcttgcgag attgccaact gaatggattc atcatccatg ggacgctcct ttaaccgtac
     6481 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca
     6541 cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag atcatgatcg
     6601 gagcagcagg tggaggcggt tcaggcggag gtggcagcgg cgtgagcaag ggcgaggagg
     6661 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     6721 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     6781 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     6841 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     6901 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     6961 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     7021 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     7081 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     7141 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     7201 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     7261 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     7321 ccggcggcat ggacgagctg tacaaggctc gaggcgcagc agcgggagca ggaggagcag
     7381 gtcgatcggg atccggcgga tctgcagatt cagctacacc acatttggac gccgttgagc
     7441 aaacattgag gcaagtgtcc ccaggcctgg agggcgatgt atgggaaaga actagtggaa
     7501 ataaattgga cggaagtgcc gctgatccca gtgattggtt gttgcaaacc ccaggatgtt
     7561 ggggcgatga taaatgcgct gacagagttg ggacaaaaag gttgctggct aagatgactg
     7621 aaaatatagg gaatgccacc agaaccgtag atattagtac acttgcacct ttcccaaacg
     7681 gcgcattcca agacgctatt gtcgctggtt tgaaggagtc cgcagctaga ggcaataagt
     7741 tgaaagttag gatactggtt ggggctgcac ccgtctacca catgaatgta ataccttcca
     7801 agtataggga cgaattgact gcaaaacttg gcaaagccgc agaaaacata actttgaacg
     7861 tggctagtat gactacatca aaaacagctt tctcctggaa ccatagcaaa atcttggttg
     7921 tagacgggca aagcgccctt actggtggaa taaatagctg gaaggatgat tacttggata
     7981 ctactcatcc agtgtctgat gttgatcttg cactgaccgg accagctgca ggctctgccg
     8041 gtagatacct tgacactttg tggacatgga cctgccaaaa caagagtaac atcgcttctg
     8101 tctggtttgc agcttccggc aacgcagggt gcatggccac tatgcataaa gatacaaacc
     8161 ccaaggcatc acctgccacc gggaatgtcc cagtaatagc cgttggaggg ttgggcgttg
     8221 gcatcaaaga tgtggacccc aagtccactt tcaggcccga tctgccaaca gctagcgata
     8281 ctaagtgtgt ggtaggactt cacgacaata ctaacgccga tagagactat gatacagtca
     8341 acccagaaga gagtgccttg agagctcttg ttgcttcagc aaaaagtcac attgaaatat
     8401 ctcagcagga tttgaacgct acatgtccac ccttgcctag gtatgacatt agattgtacg
     8461 atgccttggc tgctaaaatg gctgctgggg ttaaggtgag aattgtggtt agcgacccag
     8521 ccaacagggg cgcagtgggc agtgtgggtt acagccaaat aaaatcactt agtgaaatat
     8581 cagacacttt gagaaacagg cttgcaaata tcacaggcgg tcaacaagct gccaaaactg
     8641 caatgtgtag caatttgcag cttgcaacct ttagatcctc ccccaacgac aaatgggcag
     8701 acgggcatcc ttatgcccaa caccataagt tggtttccgt cgactcatcc acattttata
     8761 tcggttcaaa aaatttgtac ccatcctggc tgcaagattt tggctacata gtggaatccc
     8821 cagaggctgc caagcaactg gatgccaaat tgcttgaccc tcagtggaag tactcacagg
     8881 agactgcaac agtggattac gcaagaggaa tctgcaacgc tgaattctaa cgcggccgca
     8941 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     9001 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     9061 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     9121 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     9181 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     9241 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     9301 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     9361 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     9421 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     9481 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9541 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9601 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9661 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     9721 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9781 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9841 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9901 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9961 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    10021 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    10081 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
    10141 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
    10201 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
    10261 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
    10321 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
    10381 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
    10441 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
    10501 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
    10561 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
    10621 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
    10681 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    10741 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt
    10801 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    10861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10981 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    11041 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    11101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11161 tgtccaaact catcaatgta tcttatcatg t
//
