LOCUS       sequence-37173-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    145..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     236..255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(287..320)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    328..361
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             390..419
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             429..458
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             468..497
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             507..536
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             546..575
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             609..638
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggannncggc atggacgagc tgtacaagta atctagagga tcataatcag ccataccaca
       61 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gaattcataa cttcgtatag
      301 catacattat acgaagttat aggatccgaa gttcctattc tctagaaagt ataggaactt
      361 cccgggccta ggatcgattt aaagctatgg agcaaaagct catttctgaa gaggacttga
      421 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
      481 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
      541 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagagc ttgggcgacc
      601 tcaccatgga gcaaaagctc atttctgaag aggacttgaa ttcaaggcct ctcgaagctt
      661 ccatggtgtc caagctcacg tctctccagc aagaactcct gagtgccctg ctgagctccg
      721 gggtcaccaa ggaggtgctg gttcaggcct tggaggagtt gctgccatcc ccgaacttcg
      781 gggtgaagct ggagacgctg cccctgtccc ctggcagcgg ggccgagccc gacaccaagc
      841 cggtcttcca tactctcacc aacggccacg ccaagggccg ct
//
