LOCUS       sequence-37174-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: P2_DD-HNF4A8_C106R
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(94..96,97..417)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             433..462
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             472..501
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             511..540
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             550..579
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             589..618
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             652..681
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctnttttgac tccntanaag acaccgggac cgatccagcc tccggactct agcgtttaaa
       61 cttaagcttg gtaccgagct cggatccgcc accatgggag tgcaggtgga aaccatctcc
      121 ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gtgtggtgca ctacaccggg
      181 atgcttgaag atggaaagaa agtcgattcc tcccgggaca gaaacaagcc ctttaagttt
      241 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
      301 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca
      361 ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa accggaagaa
      421 ttgcgcgcta tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
      481 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
      541 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc
      601 atttctgaag aggacttgaa tgaaatggag agcttgggcg acctcaccat ggagcaaaag
      661 ctcatttctg aagaggactt gaattcaagg cctggtaccc aattcgtcag cgtgaacgcg
      721 cccctcgggg ctccagtgga gagttcttac gacacgtccc catcagaagg caccaacctc
      781 aacgcgccca acagcctggg tgtcagcgcc ctgtgtgcca tctgcgggga ccgggccacg
      841 ggcaaacact acggtgcctc gagctgtgac ggcctgcaag ggcttcttcc ggaggagcgt
      901 gcgtgaactn tcggaaccng aagc
//
