LOCUS       sequence-37178-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE
  TITLE     DelsGate, a robust and rapid gene deletion construction method.
  JOURNAL   Fungal Genet Biol. 2008 Apr . 45(4):379-88.
  PUBMED    18248826
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2008 Apr . 45(4):379-88."
COMMENT     Sequence Label: pDONR-A-HYG
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      198..284
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      376..403
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(618..637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
       61 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
      121 actcgtccga gggcaaagga atagagtaga tgccgaccgg gaaccagtta acgcgagagt
      181 agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt
      241 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
      301 tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
      361 ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
      421 ttcctggcta gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgcctt
//
