LOCUS       sequence-371821-        9864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of Zyxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Hoffman LM, Jensen CC, Chaturvedi A, Yoshigi M, Beckerle MC
  TITLE     Stretch-induced actin remodeling requires targeting of zyxin to 
            stress fibers and recruitment of actin regulators.
  JOURNAL   Mol Biol Cell. 2012 May;23(10):1846-59. doi: 
            10.1091/mbc.E11-12-1057. Epub 2012 Mar 28.
  PUBMED    22456508
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-12-1057"; journalName: "Mol Biol Cell"; date: 
            "2012-05"; volume: "23"; issue: "10"; pages: "1846-59"
COMMENT     Sequence Label: pLenti-Zyxin-G (aa1-372)-EGFP
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3712..3732
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(3735..3737,3738..3740,3741..4451)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3735..3737,3738..3740,3741..4451)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3780..3801)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4041..4060)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4388..4409
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4505..4523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4528..4662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4725..4749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4802..4843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4867..5455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4920..4940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5338..5349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5470..5786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5637..5772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5834..5881
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5900..6298
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6385..6618
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6690..6811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6851..6986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7007..7025)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7035..7052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7035..7051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7044..7066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7193..7648
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7280..7299)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7490..7511
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7674..7778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7779..7847,7848..8639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7997..8016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8810..9398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9299..9318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9552..9569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9686..9707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9722..9727,9728..9745,9746..9752)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9760..9776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9765..9787
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9784..9800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9784..9800
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9819..9839
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9821..9839
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg cttaatggcg gccccccgcc cgcctcccgc gatctccgtc tccgtctcgg
     2641 cccccgcgtt ttacgccccg cagaagaagt tcgccccggt tgtggcccca aagcccaaag
     2701 tgaatccttt ccggcctggg gacagcgagc ctcctgtagc agccggggcc caaagagcgc
     2761 agatgggtcg ggtgggcgag atcccaccac cacccccgga agactttcct ttgccccctc
     2821 ctccccttat tggggagggc gacgactcag agggtgccct gggaggtgcc ttcccacctc
     2881 cacctccccc gatgatcgag gaaccattcc cccctgctcc tctggaggag gacatcttcc
     2941 cctcccctcc acctccactg gaggaggagg gagggcctga ggcccctacc cagctcccac
     3001 cgcagcccag ggagaaagtg tgcagtattg acctggagat tgactctctg tcctcactgc
     3061 tggacgacat gaccaagaac gatcccttca aagcccgggt atcatccgga tatgtacccc
     3121 caccagttgc cactccattt gttcccaagc ctagtaccaa acctgcccct gggggcacag
     3181 cacccttgcc tccttggaag accccttcta gctcccagcc accacctcag ccgcaggcca
     3241 agcctcaggt ccagctccat gtccagcctc aggccaagcc ccatgtccaa ccccagcctg
     3301 tgtcttctgc taatacacag ccccggggtc ccctttctca ggcaccaact ccagcaccta
     3361 agtttgctcc agtggctcct aaatttactc ccgtggtttc caagttcagc cctggtgctc
     3421 caagtggacc tgggccacag cccaatcaaa aaatggtgcc tccggatgct ccttcttctg
     3481 tgagcacagg ctcccctcag ccccctagct tcacctatgc tcagcagaag gagaagcccc
     3541 tagttcaaga gaagcagcac ccacagcctc caccagctca aaaccaaaac caggtacgct
     3601 ctcctggagg cccaggcccc ttgaccctga aggaggtaga ggagttggag cagctgaccc
     3661 agcagctgat gcaggacatg gaacaccctc agaggcagag cgtggcagtg acaactttgt
     3721 atacaaaagt tgtgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3781 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3841 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3901 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3961 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4021 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4081 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4141 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4201 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4261 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4321 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4381 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4441 agctgtacaa gggcggtgga agatctggga attcaaggcc tctcgagcct ctagattctg
     4501 cagccctata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
     4561 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4621 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4681 cattttatgt ttcaggttca gggggaggtg tgggaggttt ttttacccag ctttcttgta
     4741 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     4801 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     4861 atcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     5281 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggc
     5461 gatggtaccg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5701 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5761 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5821 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     5881 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     5941 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     6001 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     6061 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     6121 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     6181 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     6241 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
     6301 acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6361 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6421 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6601 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6661 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6721 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6781 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6841 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6901 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6961 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7021 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7081 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7141 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7201 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7261 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7321 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7381 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7441 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7501 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7561 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7621 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7681 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7741 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8461 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8641 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8701 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8941 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9001 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9061 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9121 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9181 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9241 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9301 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9361 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9421 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9481 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9541 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9601 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9661 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9721 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9781 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9841 aacaaaagct ggagctgcaa gctt
//
