LOCUS       sequence-371834-       10766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mNeptune2.5
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1488..1509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1700..1719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             4737..4748
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7216..7238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7227..7243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7251..7257,7258..7275,7276..7281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7434..7451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7605..8193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7685..7704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8364..9155,9156..9224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8987..9006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9225..9329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9356..10698
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10319..10366)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10739..10757
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgct ggagaagctc attccacccg aaatgcccgt
     1981 gaacttgccc ttgtttgcgc gggccttctt gcgctctgct cggatacgct cgacatcgct
     2041 gaggagctcg gcgagttcct tggcgcggtt gcggacgttg ataccctggt ctttgccgtt
     2101 ctggtcgata aaatggaact ggcgcagcat cttgaggagg gtaagatgcg accgagcatc
     2161 gtccaccact cgttcggaac cgtgcttaat caggtactct agtagttgaa gggccttgta
     2221 gatctggcgc cattcttcgg cggacttttc tgtgaaacgg cggtaaatca tgggcatgat
     2281 ttcgttgagt gtttggctaa gaacaaggag ggaaacgcaa gttagccgcg ttcgtataca
     2341 agcgagcagt tctcgagaac gtactagttg aaggtgccat cggcgatctc ttgcatcaaa
     2401 gtcgacgatg caccccaagg ttcattgttg gtggcctcgc gcacctgggt tcgctgtcag
     2461 ctcccgttcc atggtctctt gtctgggatc aagtatatgg cgaagggcgc aagagaatgg
     2521 tgccagtgtg gcgcgtggat acgggcatac cttggactcc atctcggtat agttcatgac
     2581 agctggttgg agccattgtc agcaccctga acacgaaatc atgagggagt cttggttctg
     2641 gactaggtgg tcacctacca ttttgaacct ttcgtacgcc agccttgatg tcgtagagtg
     2701 tcaggttgga cacggtatcc ttgaggctat tgaaatccat tgcgtaaacg tgtctatcgc
     2761 cggagttgct gggaattgtt ctgatgggct aaggtaattc ttctctagat ccaatctgag
     2821 cctcgcggtg atgattgcca gacgggcggg atgctgtggc gacttggtgg tttttgttcg
     2881 ggctgatcgc tttcagggcg acgtttcacg gtcttgtcgg gatcagatgg acattgaggt
     2941 gcggaattat acgatttagg tgactgcagg ccatacagat ctccctagac gccgggccaa
     3001 tccgttcctt gcaccttctc tcacacatgc aggttttgga atgtggcttc atcgtgtcca
     3061 gaactgggcg cattggggcc aggctcagtg tcagtgtagg gatcgatgta catatggcag
     3121 ttgacagcga gagagccagg tgtcacaggc agtccgtcag tggccgcggt ggtggccacg
     3181 cactttctgc aggagccgtc tggggtgcgt gcagctggcg gccgcttccg ggcatacggc
     3241 ttgtgtaacg agggtgttga ccctcggctt ggcgcttgaa cttggcgctg cggtcccccc
     3301 acagttgacc acaatccccg gccaatcgaa cgacggcaac aagggcgcac tagccagtga
     3361 acgcctgggg atagtgggtg gtcaaagaga attgggcaaa cgagacgaca atggagggtg
     3421 aaccgggaat aataaacagc tggtgagacc acccatcaaa cgaatcaccc caccatggga
     3481 tgccctcaga tgccctccat gccaccataa ggaatgatct tacggcttgc cgaagttaac
     3541 aggcctgctt ttgcatatga acgagagcct ggtgttgcat tccacattgg ctatctacag
     3601 tacctaccca acctacacac cctgatgcga aagggacaga aggggagaag gatacataca
     3661 tatacctgac tcgtgcccat caacaacaga gaaagggtga tcgctgaaca catcagagca
     3721 tctcttcccc cctggcgact aaaagtgctc ccaatctcgt cttacatctg atacgttgtt
     3781 caaactccgc aacccgatct gcacactgcc ctgccttggc ccttcttgga gtctccgctt
     3841 ccccacgcca tctcacgctc gaggactccg ctgctaggta tgagctaggc ctgccgtcga
     3901 caggatccac cagacgtcaa caataaccca tcttggaaat acataagaag acgtcacttc
     3961 cctccctttt cgaaaggaag agatcatcat cagcatccat catggctggt ctttttggtt
     4021 ccgccgccgc atccgcgagc aacaccctcg gcgatcttaa gcaggatgtc gagctcggcc
     4081 aaccccccga ggacagcatc agcgaccttg ccttcaaccc caaccccgcc gaccagaagg
     4141 acttcctcgc cgtagccagc tgggacaaga agacacgcat ttacgagatc ttgagtaatg
     4201 gtcaaggtca gggacaggcc atgattgagc atgatgcccc cgttttctct tgcgatttct
     4261 tcaaggtatg tttgcgtcga ttggcctggc aggtagcccg acaaaacact gacatgcact
     4321 tctcccaaca ggacggcaca aaggtcattt ccgccggcgc cgacaaggcc gccaaggttc
     4381 tcgatttggc caccggtcag tcgatgcagg tcgctgctca cgatatgccc atcaagtgcg
     4441 ttcgctactt cgaggccaat ggcacgccca tggcggtgac aggtggctgg gacaagcaga
     4501 tcaagtactg ggatttccgc tccgcgaacc cggccgccac ggtgcaggcc caggagcgcg
     4561 tttataccat ggatgtgcgc gacaacttgc tcgtggtcgg aaccgctgat cgctatatca
     4621 acgtgatcaa cctgaaggac cccggcaagt tctacaagac aatgcagagc cctcttaaat
     4681 ggcagactcg tgtggtcagc tgcttcaacg attctcaggg ctttgctatc ggctcgatcg
     4741 agggtagatg cgccattcaa tatgtggagg acaaggattc tgcgtacgcc ttcacctcct
     4801 ccccctttac tatcccatct gccgaatacg ctgactaact gacttgtttt ccagctccaa
     4861 cttctctttc aaatgccacc gcgaccccgc ccaaggcaac accacggccg tccacgctgt
     4921 caacgacatc tccttccacc cccaacacgg caccttcagc acagccggta gcgacggcac
     4981 cttccacttc tgggacaagg acgccaagca ccgtctcaag ggttacccca acgtcggcgg
     5041 cagcatcacc tcgacaacgt tcaacaagac cggcagcatc tttgcctacg ccattagtta
     5101 tgactggagc aaggggtacc aaggcaactc gcccacgtac ccgaccaagg tcatgctcca
     5161 cccagtacag caggacgagt gcaagccccg tccgagcgtt aagaagaggg ctggtattgg
     5221 tttggctatt gctggcatgg tctccaaggg cgaggagctc atcaaggaga acatgcacac
     5281 caagctctac atggagggca ccgtcaacaa ccaccacttc aagtgcaccc acgagggcga
     5341 gggcaagccc tacgagggca cccagaccaa ccgcatcaag gtcgtcgagg gcggccccct
     5401 ccccttcgcc ttcgacatcc tcgccacctg cttcatgtac ggctccaaga ccttcatcaa
     5461 ccacacccag ggcatccccg acttcttcaa gcagtccttc cccgagggct tcacctggga
     5521 gcgcgtcacc acctacgagg acggcggcgt cctcaccgtc acccaggaca cctccctcca
     5581 ggacggctgc ctcatctaca acgtcaagct ccgcggcgtc aacttcccct ccaacggccc
     5641 cgtcatgcag aagaagaccc tcggctggga ggcctccacc gagaccctct accccgccga
     5701 cggcggcctc gagggccgct gcgacatggc cctcaagctc gtcggcggcg gccacctcca
     5761 ctgcaacctc aagaccacct accgctccaa gaagcccgcc aagaacctca agatgcccgg
     5821 cgtctacttc gtcgaccgcc gcctcgagcg catcaaggag gccgacaacg agacctacgt
     5881 cgagcagcac gaggtcgccg tcgcccgcta ctgcgacctc ccctccaagc tcggccacaa
     5941 gctcaacggc atggacgagc tctacaagtg actagaggat ccgacactat agaataccta
     6001 gacagtttgc aggtcatgtg aatgttatcg ccaacacatt gaatgctaaa cagattacct
     6061 ccaacgccta gatgaaacca aattaccctt gccttctttt tttttacgcc gttaatgcag
     6121 ttgtgataga gaccatgcaa cgtcttctaa acggagacac aaacacattc gagtcgatca
     6181 gatcgaaatg acttgttcct gtacctcgtc accatccagc caccgtctga aatagacgca
     6241 gttgctgggg ctgacaggtc cgccttcctc gcacatatca tagacagggc accgcccaca
     6301 aggcgcctcc ataaggccac ttgcactcgg ctcaactccc ataaatgagg taacaatgcc
     6361 tcctttggtt ggatcatggt cgaggcgtcg ttgattgcac gtcctcggat cctgtttggg
     6421 gatgcgcgtc acacggtacc ccttgcgccc attgaccttg acaggctcta ctaagccatc
     6481 gtagtatagc acatccacca actgctggat atccgcctcg ctcaacgtcg tattattcgt
     6541 gatgccggcg tggtggatga actttgcaat gtccgacaca gtcgggtacg acttgtaacc
     6601 ggccgggaga ggcagcaatt gcggcttgcc gggcctggtc ttcttggccg gtgccggtgc
     6661 cgccgtgtcg tcgttggaaa tctcgcccgc gctgcgcttc gtccccttgg aaatatcctc
     6721 cgagtgggga atggtcccct gaaccacgcc cttcttggga ttcttttcac gctgctgtgc
     6781 tcctcctcct tgtgctccta ctcctggtac tccagcaccg ccgccgtggg aagtatatgt
     6841 cgatttctgc ttgataaagt caaacacaat ttcctgtagc tcgttgatga aggccgtgtc
     6901 gagctcgccc tcagtaaacc aggggccacc tgtggcgcga tcggagggac gtaggttcgc
     6961 cttgatgtac atcttcttgt tggggtgctc gacgttcttc atgctggcga tgtagccctt
     7021 ttgctcgaga tacttgatgc aatgcttcat aagcgagtcg gccatgttga gcttgttctt
     7081 gatggtccgg ttccagatgc cgtcggcccc ggcttcgtcg accatggcgt ataccatcat
     7141 tatttcggag ttggggaggg aggtgtacct tttgggagga aggggaggtt agttggtgtg
     7201 ctcggttggc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
     7261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     7321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8041 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
     9361 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     9421 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     9481 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     9541 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     9601 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     9661 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     9721 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     9781 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     9841 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     9901 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     9961 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
    10021 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
    10081 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
    10141 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
    10201 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
    10261 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
    10321 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
    10381 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
    10441 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
    10501 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
    10561 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
    10621 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
    10681 ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
    10741 taggcgtatc acgaggccct ttcgtc
//
