LOCUS       sequence-37186-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM
  TITLE     Activation of endogenous Cdc42 visualized in living cells.
  JOURNAL   Science. 2004 Sep 10. 305(5690):1615-9.
  PUBMED    15361624
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Sep 10. 305(5690):1615-9."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42(Q61L)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(23..202,203..205,206..598)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(626..644)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(627..644)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(664..681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    670..781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 caggacgagc tgtcaggaat tcatgcagac aattaagtgt gttgttgtgg gcgatggtgc
       61 tgttggtaaa acatgtctcc tgatatccta cacaacaaac aaatttccat cggaatatgt
      121 accgactgtt tttgacaact atgcagtcac agttatgatt ggtggagaac catatactct
      181 tggacttttt gatactgcag ggctagagga ttatgacaga ttacgaccgc tgagttatcc
      241 acaaacagat gtatttctag tctgtttttc agtggtctct ccatcttcat ttgaaaacgt
      301 gaaagaaaag tgggtgcctg agataactca ccactgtcca aagactcctt tcttgcttgt
      361 tgggactcaa attgatctca gagatgaccc ctctactatt gagaaacttg ccaagaacaa
      421 acagaagcct atcactccag agactgctga aaagctggcc cgtgacctga aggctgtcaa
      481 gtatgtggag tgttctgcac ttacacagaa aggcctaaag aatgtatttg acgaagcaat
      541 attggctgcc ctggagcctc cagaaccgaa gaagagccgc aggtgtgtgc tgctatgact
      601 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      661 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      721 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      781 cgcattgtct gagtaggtgt cattctattc tgggggggtg gggtggggca ggacagcaag
//
