LOCUS       sequence-37190-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Sauert K, Kahnert S, Roose M, Gull M, Brandli AW, Ryffel GU, Waldner
            C
  TITLE     Heat-shock mediated overexpression of HNF1beta mutations has 
            differential effects on gene expression in the Xenopus pronephric 
            kidney.
  JOURNAL   PLoS One. 2012;7(3):e33522. Epub 2012 Mar 15.
  PUBMED    22438943
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33522. Epub 2012 Mar 15"
COMMENT     Sequence Label: pLCMV:ECFP(loxP)(FRT)HNF1B
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    136..270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(173..192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     227..246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(278..311)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    319..352
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             381..410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             420..449
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             459..488
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             498..527
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             537..566
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             600..629
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcnggacgag ctgtacaagt aatctagagg atcataatca gccataccac atttgtagag
       61 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      121 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      181 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      241 ctcatcaatg tatcttatca tgtctggatc tgaattcata acttcgtata gcatacatta
      301 tacgaagtta taggatccga agttcctatt ctctagaaag tataggaact tcccgggcct
      361 aggatcgatt taaagctatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      421 agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag
      481 aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc
      541 aaaagctcat ttctgaagag gacttgaatg aaatggagag cttgggcgac ctcaccatgg
      601 agcaaaagct catttctgaa gaggacttga attcaaggcc tctcgaagct tccatggtgt
      661 ccaagctcac gtctctccag caagaactcc tgagtgccct gctgagctcc ggggtcacca
      721 aggaggtgct ggttcaggcc ttggaggagt tgctgccatc cccgaacttc ggggtgaagc
      781 tggagacgct gcccctgtcc cctggcagcg gggccgagcc cgacaccaag ccggtcttcc
      841 atactctcac caacggccac gccaagggcc gctgcgaagc ag
//
