LOCUS       sequence-37191-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sauert K, Kahnert S, Roose M, Gull M, Brandli AW, Ryffel GU, Waldner
            C
  TITLE     Heat-shock mediated overexpression of HNF1beta mutations has 
            differential effects on gene expression in the Xenopus pronephric 
            kidney.
  JOURNAL   PLoS One. 2012;7(3):e33522. Epub 2012 Mar 15.
  PUBMED    22438943
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33522. Epub 2012 Mar 15"
COMMENT     Sequence Label: pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(160..179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     214..233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(265..298)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    306..339
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             368..397
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             407..436
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             446..475
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             485..514
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             524..553
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             587..616
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtcaagtaat ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
       61 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      121 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      181 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      241 cttatcatgt ctggatctga attcataact tcgtatagca tacattatac gaagttatag
      301 gatccgaagt tcctattctc tagaaagtat aggaacttcc cgggcctagg atcgatttaa
      361 agctatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat
      421 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      481 aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc
      541 tgaagaggac ttgaatgaaa tggagagctt gggcgacctc accatggagc aaaagctcat
      601 ttctgaagag gacttgaatt caaggcctct cgaagcttcc atggtgtcca agctcacgtc
      661 tctccagcaa gaactcctga gtgccctgct gagctccggg gtcaccaagg aggtgctggt
      721 tcaggccttg gaggagttgc tgccatcccc gaacttcggg gtgaagctgg agacgctgcc
      781 cctgtcccct ggcagcgggg ccgagcccga caccaagccg gtcttccata ctctcaccaa
      841 cggccacgcc
//
