LOCUS       sequence-37192-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of oChIEF-tdTomato for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Bell KA, Shim H, Chen CK, McQuiston AR
  TITLE     Nicotinic excitatory postsynaptic potentials in hippocampal CA1 
            interneurons are predominantly mediated by nicotinic receptors that 
            contain alpha4 and beta2 subunits.
  JOURNAL   Neuropharmacology. 2011 Aug 25.
  PUBMED    21878344
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2011 Aug 25."
COMMENT     Sequence Label: AAV-Flex-rev-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..126
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(230..253)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(428..447)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     697..715
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cttcgtataa agtatcctat acgaagttat atcaaaatag gaagaccaat gcttcaccat
       61 cgacccgaat tgccaagcat caccatcgac ccataacttc gtataatgta tgctatacga
      121 agttatacta ggatcccctc gagctacttg tacagctcgt ccatgccgta caggaacagg
      181 tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      241 gtgatgtcca gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc
      301 atgtagatgg tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg
      361 tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag
      421 cccatggtct tcttctgcat tacggggccg tcggggggga agttggtgcc gcgcatcttc
      481 accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct gggtcacggt caccagaccg
      541 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg
      601 taatcgggga tgtcggcggg gtgcttcacg tacgccttgg agccgtacat gaactggggg
      661 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc
      721 tg
//
