LOCUS       sequence-371926-        2680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty pSEVA63x vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Pathania A, Hopper C, Pandi A, Fugger M, Nowak T, Kushwaha M
  TITLE     A synthetic communication system uncovers extracellular immunity 
            that self-limits bacteriophage transmission
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: pSEVA63x
FEATURES             Location/Qualifiers
     source          1..2680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(5..10,11..27,28..33)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             222..755
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            966..1074
                     /note="incP origin of transfer"
     CDS             complement(1085..1747)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      1748..2519
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 aaatttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg
       61 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
      121 ggcttgttat gactgttttt ttgtacagcc tatgcctcgg gcatccaagc agcaagcgcg
      181 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg
      241 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca
      301 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
      361 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc
      421 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
      481 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct
      541 atatctatga tctcgcagtc tccggagagc accggaggca gggcattgcc accgcgctca
      601 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
      661 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
      721 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct
      781 tcccggccgc ggagttgttc ggtaaattgg acaacggtcc gcgcgttgtc cttttccgct
      841 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
      901 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
      961 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     1021 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc
     1081 ggccctaccg gcgcggcagc gttacccgtg tcggcggctc caacggctcg ccatcgtcca
     1141 gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg
     1201 tttcggtcaa ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct
     1261 cctcgccggg gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca
     1321 ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac
     1381 tgaacaccga caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca
     1441 cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca
     1501 ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg
     1561 tcttctggct gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca
     1621 gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt
     1681 ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg
     1741 tggccatgct tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca
     1801 gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta
     1861 cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg
     1921 tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt
     1981 ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc
     2041 cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg
     2101 cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca
     2161 ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg
     2221 cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg
     2281 tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta
     2341 aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga
     2401 aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc
     2461 atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct
     2521 cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
     2581 agcccggaga gcaagcccgt agggggggcg cgagattgat ttaccccggt tgataatcag
     2641 aaaagcccca aaaacaggaa gattgtataa gcaaatattt
//
