LOCUS       sequence-371938-       17866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation
            factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17866)
  AUTHORS   Bai M, Yuan J, Kuang H, Gong P, Li S, Zhang Z, Liu B, Sun J, Yang M,
            Yang L, Wang D, Song S, Guan Y
  TITLE     Generation of a multiplex mutagenesis population via pooled 
            CRISPR-Cas9 in soya bean.
  JOURNAL   Plant Biotechnol J. 2020 Mar;18(3):721-731. doi: 10.1111/pbi.13239. 
            Epub 2019 Sep 9.
  PUBMED    31452351
REFERENCE   2  (bases 1 to 17866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13239";
            journalName: "Plant Biotechnol J"; date: "2020-03"; volume: "18"; 
            issue: "3"; pages: "721-731"
COMMENT     Sequence Label: pGES201
FEATURES             Location/Qualifiers
     source          1..17866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(618..623,624..641,642..648)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             702..1361
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(768..787)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1703..2008
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1887..1906
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2049..2173)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2200..2452
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        2460..2535
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4129..4194
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4201..4221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4246..8346
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4246..8346
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8347..8394
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8433..8685
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8702..8718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8702..8718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8715..8737)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8726..8742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8750..8756,8757..8774,8775..8780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8795..8816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9007..9352
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9295..9315
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9425..9976
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPSDIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    10017..10191
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10269..10293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10718..11512
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12937..13525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13426..13445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13679..13696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13711..13851
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13777..13796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13937..13959
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14195..14389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14455..15528)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15957..16586)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttaaaataa atggtaaaat gtcaaatcaa
      301 aactaggctg cagtatgcag agcagagtca tgatgatact acttactaca ccgattcttg
      361 tgtgcagaaa aatatgttaa aataattgaa tctttctcta gccaaatttg acaacaatgt
      421 acaccgttca tattgagaga cgatgcttct tgtttgcttt cggtggaagc tgcatatact
      481 caacattact ccttcagcga gttttccaac tgagtcccac attgcccaga cctaacacgg
      541 tattcttgtt tataatgaaa tgtgccacca catggattga gacctgtgag ttagctcact
      601 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      661 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      721 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      781 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      841 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      901 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      961 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1021 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1081 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1141 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1201 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1261 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     1321 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac
     1381 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     1441 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     1501 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatatccggt
     1561 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     1621 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     1681 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     1741 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     1801 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     1861 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     1921 gtccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     1981 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     2041 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     2101 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     2161 tgtacaaagt ggtgatctag agggagctcg aatttccccg atcgttcaaa catttggcaa
     2221 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2281 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2341 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2401 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggtctctg
     2461 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     2521 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
     2581 ccgtagcgcg tgcgccaatt ctgcagacaa atggccccgg gtttacttat tttgtgggta
     2641 tctatacttt tattagattt ttaatcaggc tcctgatttc tttttatttc gattgaattc
     2701 ctgaacttgt attattcagt agatcgaata aattataaaa agataaaatc ataaaataat
     2761 attttatcct atcaatcata ttaaagcaat gaatatgtaa aattaatctt atctttattt
     2821 taaaaaatca tataggttta gtattttttt aaaaataaag ataggattag ttttactatt
     2881 cactgcttat tacttttaaa aaaatcataa aggtttagta tttttttaaa ataaatatag
     2941 gaatagtttt actattcact gctttaatag aaaaatagtt taaaatttaa gatagtttta
     3001 atcccagcat ttgccacgtt tgaacgtgag ccgaaacgat gtcgttacat tatcttaacc
     3061 tagctgaaac gatgtcgtca taatatcgcc aaatgccaac tggactacgt cgaacccaca
     3121 aatcccacaa agcgcgtgaa atcaaatcgc tcaaaccaca aaaaagaaca acgcgtttgt
     3181 tacacgctca atcccacgcg agtagagcac agtaaccttc aaataagcga atggggcata
     3241 atcagaaatc cgaaataaac ctaggggcat tatcggaaat gaaaagtagc tcactcaata
     3301 taaaaatcta ggaaccctag ttttcgttat cactctgtgc tccctcgctc tatttctcag
     3361 tctctgtgtt tgcggctgag gattccgaac gagtgacctt cttcgtttct cgcaaaggta
     3421 acagcctctg ctcttgtctc ttcgattcga tctatgcctg tctcttattt acgatgatgt
     3481 ttcttcggtt atgttttttt atttatgctt tatgctgttg atgttcggtt gtttgtttcg
     3541 ctttgttttt gtggttcagt tttttaggat tcttttggtt tttgaatcga ttaatcggaa
     3601 gagattttcg agttatttgg tgtgttggag gtgaatcttt tttttgaggt catagatctg
     3661 ttgtatttgt gttataaaca tgcgactttg tatgattttt tacgaggtta tgatgttctg
     3721 gttgttttat tatgaatctg ttgagacaga accatgattt ttgttgatgt tcgtttacac
     3781 tattaaaggt ttgttttaac aggattaaaa gttttttaag catgttgaag gagtcttgta
     3841 gatatgtaac cgtcgatagt ttttttgtgg gtttgttcac atgttatcaa gcttaatctt
     3901 ttactatgta tgcgaccata tctggatcca gcaaaggcga ttttttaatt ccttgtgaaa
     3961 cttttgtaat atgaagttga aattttgtta ttggtaaact ataaatgtgt gaagttggag
     4021 tataccttta ccttcttatt tggctttgtg atagtttaat ttatatgtat tttgagttct
     4081 gacttgtatt tctttgaatt gattctagtt taagtaatcc tcgagatgga ctataaggac
     4141 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     4201 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     4261 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     4321 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     4381 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     4441 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     4501 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     4561 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     4621 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     4681 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     4741 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     4801 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     4861 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     4921 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     4981 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     5041 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     5101 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     5161 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     5221 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     5281 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     5341 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     5401 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     5461 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     5521 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     5581 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     5641 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     5701 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     5761 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     5821 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     5881 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     5941 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     6001 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     6061 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     6121 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     6181 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     6241 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     6301 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     6361 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     6421 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     6481 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     6541 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     6601 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     6661 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     6721 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     6781 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     6841 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     6901 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     6961 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     7021 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     7081 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     7141 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     7201 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     7261 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     7321 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     7381 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     7441 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     7501 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     7561 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     7621 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     7681 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     7741 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     7801 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     7861 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     7921 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     7981 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     8041 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     8101 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     8161 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     8221 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     8281 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     8341 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagga
     8401 tcctgattga tcgatagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     8461 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     8521 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     8581 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     8641 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttcgtaatca
     8701 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8761 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8821 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8881 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     8941 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     9001 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     9061 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     9121 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     9181 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     9241 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     9301 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     9361 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatccg gggggcaatg
     9421 agatatgagc ccagaacgac gcccgtccga catccgccgt gccaccgagg cggacatgcc
     9481 ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact tccgtaccga
     9541 gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc gctatccctg
     9601 gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggc
     9661 acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc gccaccagcg
     9721 gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg cacagggctt
     9781 caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc
     9841 gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg ggaactggca
     9901 tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc cggtcctgcc
     9961 cgtcaccgag atttgaagta gatgccgacc gggatctgtc gatcgacaag ctcgagtttc
    10021 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggttttgc
    10081 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    10141 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
    10201 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    10261 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    10321 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    10381 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    10441 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    10501 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
    10561 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
    10621 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
    10681 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
    10741 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    10801 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    10861 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    10921 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    10981 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    11041 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    11101 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    11161 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
    11221 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    11281 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    11341 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
    11401 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    11461 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
    11521 atgcatgacc aaaatccctt aacgctgatg aatcccctaa tgatttttat caaaatcatt
    11581 aagttaaggt agatacacat cttgtcatat gatcaaatgg tttcgccaaa aatcaataat
    11641 cagacaacaa aatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg
    11701 aattacactt aaaacgactc aacagcttaa cgttggcttg ccacgcctta cttgactgta
    11761 aaactctcac tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata
    11821 acatcaaacg aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg
    11881 tataccgttg gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg
    11941 actggtctga tatccgtgag caaaaacggc ttatggtatt gcgagcttca gtcgcactac
    12001 acggtcgttc tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga
    12061 aagctcatga ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc
    12121 tcattgtcag tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt
    12181 ggtactggtt aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact
    12241 ggaaacctat cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata
    12301 agaggctgac taaaagcaat ccaatctcat gccaaattct attgtataaa tctcgctcta
    12361 aaggccgaaa aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct
    12421 actcagcgtc ggcaaaggag ccatggattc tagcaactaa cttacctgtt gaaattcgaa
    12481 cacccaaaca acttgttaat atctattcga agcgaatgca gattgaagaa accttccgag
    12541 acttgaaaag tcctgcctac ggactaggcc tacgccatag ccgaacgagc agctcagagc
    12601 gttttgatat catgctgcta atcgccctga tgcttcaact aacatgttgg cttgcgggcg
    12661 ttcatgctca gaaacaaggt tgggacaagc acttccaggc taacacagtc agaaatcgaa
    12721 acgtactctc aacagttcgc ttaggcatgg aagttttgcg gcattctggc tacacaataa
    12781 caagggaaga cttactcgtg gctgcaaccc tactagctca aaatttattc acacatggtt
    12841 acgctttggg gaaattatga ggggatctct cagcccttaa cgtgagtttt cgttccactg
    12901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    12961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    13021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    13081 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    13141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    13201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    13261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    13321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    13381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    13441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    13501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    13561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    13621 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    13681 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
    13741 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    13801 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
    13861 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    13921 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    13981 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
    14041 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
    14101 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
    14161 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
    14221 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
    14281 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
    14341 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaacagacc
    14401 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
    14461 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
    14521 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
    14581 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
    14641 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
    14701 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
    14761 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
    14821 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
    14881 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
    14941 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
    15001 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    15061 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    15121 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    15181 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    15241 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    15301 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    15361 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    15421 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    15481 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    15541 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
    15601 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    15661 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    15721 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    15781 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    15841 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    15901 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    15961 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    16021 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    16081 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    16141 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    16201 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    16261 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    16321 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    16381 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    16441 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    16501 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    16561 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    16621 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    16681 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    16741 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    16801 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    16861 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    16921 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    16981 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    17041 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    17101 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    17161 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    17221 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    17281 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    17341 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    17401 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    17461 cagcttggtg cagccggccc cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    17521 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    17581 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    17641 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    17701 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    17761 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    17821 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaa
//
