LOCUS       sequence-371946-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo visualization of actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Smith MA, Blankman E, Deakin NO, Hoffman LM, Jensen CC, Turner CE, 
            Beckerle MC
  TITLE     LIM domains target actin regulators paxillin and zyxin to sites of 
            stress fiber strain.
  JOURNAL   PLoS One. 2013 Aug 21;8(8):e69378. doi: 
            10.1371/journal.pone.0069378. eCollection 2013.
  PUBMED    23990882
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-21"; volume: "8(8):e69378. doi"; pages: " 
            10.1371/journal.pone.0069378. eCollection 2013"
COMMENT     Sequence Label: pLenti-Lifeact EGFP
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..108
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    132..720
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(185..205)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(603..614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        735..1051
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      902..1037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1099..1146
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1165..1563
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1650..1883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1955..2076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2116..2251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2272..2290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2300..2317)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2300..2316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2309..2331)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2458..2913
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2545..2564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2755..2776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2939..3043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3044..3112,3113..3904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3262..3281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4075..4663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4564..4583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4817..4834
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4951..4972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4987..4992,4993..5010,5011..5017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5025..5041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5030..5052
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5049..5065
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5049..5065
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5084..5104
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5086..5104
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5132..5358
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5359..5539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5586..5711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6204..6437
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6622..6666
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6933..7050
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7073..7376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7377..7580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7531..7551
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7577..7601
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7697..7721
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7727..7777
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             join(7778..7780,7781..7783,7784..8494)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7778..7780,7781..7783,7784..8494)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8084..8103)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8431..8452
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(8548..8566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8571..8705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(join(8768..8778,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
       61 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag
      121 taatgatcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
      181 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
      241 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
      301 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
      361 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
      421 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
      481 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
      541 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
      601 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
      661 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
      721 ctggcgatgg taccggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      781 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      841 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      901 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
      961 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1021 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     1081 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1141 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc
     1201 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     1261 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     1321 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     1381 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     1441 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     1501 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     1561 taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     1621 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     1681 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1861 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     1921 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     2101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2221 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     2281 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2881 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4741 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4801 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4861 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4921 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4981 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5041 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     5101 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     5161 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     5221 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     5281 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     5341 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5521 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5581 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     5641 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     5701 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     5761 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     5821 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     5881 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     5941 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6001 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     6061 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6121 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     6181 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     6241 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     6301 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     6361 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     6421 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6481 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6541 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6601 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6661 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     6721 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6781 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6841 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     6901 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     6961 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     7021 caaaaacaaa ttacaaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
     7081 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7141 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7201 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7261 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7321 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7381 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7441 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7501 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7561 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     7621 gctgttttga cctccataga agacaccgac tctagaggat ccactagtcc agtgtggtgg
     7681 aattctgcag atatcaacaa gtttgtacaa aaaagcaggc ttaaccatgg gtgtcgcaga
     7741 tttgatcaag aaattcgaaa gcatctcaaa ggaagaaatg gtgagcaagg gcgaggagct
     7801 gttcaccggg gtggtgccca tcctggtcga gctggatggc gacgtaaacg gccacaagtt
     7861 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7921 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7981 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8041 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8101 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8161 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8221 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     8281 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8341 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     8401 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8461 cgggatcact ctcggcatgg acgagctgta caagggcggt ggaagatctg ggaattcaag
     8521 gcctctcgag cctctagatt ctgcagccct atagtgagtc gtattacgta gatccagaca
     8581 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     8641 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     8701 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     8761 tttttttacc cagctttc
//
