LOCUS       sequence-371952-       10440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of Zyxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Hoffman LM, Jensen CC, Chaturvedi A, Yoshigi M, Beckerle MC
  TITLE     Stretch-induced actin remodeling requires targeting of zyxin to 
            stress fibers and recruitment of actin regulators.
  JOURNAL   Mol Biol Cell. 2012 May;23(10):1846-59. doi: 
            10.1091/mbc.E11-12-1057. Epub 2012 Mar 28.
  PUBMED    22456508
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-12-1057"; journalName: "Mol Biol Cell"; date: 
            "2012-05"; volume: "23"; issue: "10"; pages: "1846-59"
COMMENT     Sequence Label: pLenti-Zyxin-Full Length (aa1-564)-EGFP
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4288..4308
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(4311..4313,4314..4316,4317..5027)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4311..4313,4314..4316,4317..5027)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4356..4377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4617..4636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4964..4985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5081..5099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5104..5238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5301..5325)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5378..5419
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5443..6031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5496..5516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5914..5925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6046..6362
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6213..6348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6410..6457
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6476..6874
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6961..7194
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7266..7387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7427..7562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7583..7601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7611..7628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7611..7627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7620..7642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7769..8224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7856..7875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8066..8087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8250..8354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8355..8423,8424..9215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8573..8592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9386..9974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9875..9894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10128..10145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10262..10283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10298..10303,10304..10321,10322..10328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10336..10352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10341..10363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10360..10376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10360..10376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10395..10415
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10397..10415
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg cttaatggcg gccccccgcc cgcctcccgc gatctccgtc tccgtctcgg
     2641 cccccgcgtt ttacgccccg cagaagaagt tcgccccggt tgtggcccca aagcccaaag
     2701 tgaatccttt ccggcctggg gacagcgagc ctcctgtagc agccggggcc caaagagcgc
     2761 agatgggtcg ggtgggcgag atcccaccac cacccccgga agactttcct ttgccccctc
     2821 ctccccttat tggggagggc gacgactcag agggtgccct gggaggtgcc ttcccacctc
     2881 cacctccccc gatgatcgag gaaccattcc cccctgctcc tctggaggag gacatcttcc
     2941 cctcccctcc acctccactg gaggaggagg gagggcctga ggcccctacc cagctcccac
     3001 cgcagcccag ggagaaagtg tgcagtattg acctggagat tgactctctg tcctcactgc
     3061 tggacgacat gaccaagaac gatcccttca aagcccgggt atcatccgga tatgtacccc
     3121 caccagttgc cactccattt gttcccaagc ctagtaccaa acctgcccct gggggcacag
     3181 cacccttgcc tccttggaag accccttcta gctcccagcc accacctcag ccgcaggcca
     3241 agcctcaggt ccagctccat gtccagcctc aggccaagcc ccatgtccaa ccccagcctg
     3301 tgtcttctgc taatacacag ccccggggtc ccctttctca ggcaccaact ccagcaccta
     3361 agtttgctcc agtggctcct aaatttactc ccgtggtttc caagttcagc cctggtgctc
     3421 caagtggacc tgggccacag cccaatcaaa aaatggtgcc tccggatgct ccttcttctg
     3481 tgagcacagg ctcccctcag ccccctagct tcacctatgc tcagcagaag gagaagcccc
     3541 tagttcaaga gaagcagcac ccacagcctc caccagctca aaaccaaaac caggtacgct
     3601 ctcctggagg cccaggcccc ttgaccctga aggaggtaga ggagttggag cagctgaccc
     3661 agcagctgat gcaggacatg gaacaccctc agaggcagag cgtggcagtg aatgagtcct
     3721 gtggcaaatg caatcagcca ctggcccgtg cacagcctgc ggttcgtgca ctgggacaac
     3781 tgttccacat cacctgcttc acttgccatc agtgtcagca gcagctgcag ggacagcagt
     3841 tctatagcct ggagggagca ccatattgtg agggctgcta caccgacact ttggagaagt
     3901 gcaacacctg tgggcagccc atcactgacc gcatgctgag ggccactggc aaagcctacc
     3961 acccacagtg cttcacctgt gtggtctgcg cctgtcccct ggagggcacc tccttcattg
     4021 tggaccaggc caatcagccc cactgtgtcc ctgactatca caagcaatac gctccaaggt
     4081 gctccgtctg ctcggagcca atcatgcctg agcctggccg agacgagact gtgcgagtag
     4141 tggcgctgga taagaacttt catatgaagt gttacaagtg tgaggactgt gggaaacctc
     4201 tgtccattga ggcagatgac aacggctgtt tccctctgga tggccacgtc ctttgtcgga
     4261 agtgccactc cgctagagcc cagaccacaa ctttgtatac aaaagttgtg atggtgagca
     4321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggtggaagat
     5041 ctgggaattc aaggcctctc gagcctctag attctgcagc cctatagtga gtcgtattac
     5101 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5161 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5221 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5281 gaggtgtggg aggttttttt acccagcttt cttgtacaaa gtggttgata tccagcacag
     5341 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     5401 cctcggtctc gattctacgc gtaccggtta gtaatgatcg acaatcaacc tctggattac
     5461 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5521 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5581 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5641 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5701 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5761 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5821 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5881 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5941 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6001 agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc
     6061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6181 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6241 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6301 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6361 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6421 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     6481 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     6541 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     6601 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     6661 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     6721 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     6781 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     6841 attgctgccc tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa
     6901 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6961 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7261 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7381 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7561 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7741 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7981 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8101 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8221 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10381 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
