LOCUS       sequence-371970-        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the monomeric form of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Teige LH, Kumar S, Johansen GM, Wessel O, Vendramin N, Lund M, 
            Rimstad E, Boysen P, Dahle MK
  TITLE     Detection of Salmonid IgM Specific to the Piscine Orthoreovirus 
            Outer Capsid Spike Protein Sigma 1 Using Lipid-Modified Antigens in 
            a Bead-Based Antibody Detection Assay.
  JOURNAL   Front Immunol. 2019 Sep 6;10:2119. doi: 10.3389/fimmu.2019.02119. 
            eCollection 2019.
  PUBMED    31552049
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2019-09-6"; volume: "10:2119. doi"; pages: " 
            10.3389/fimmu.2019.02119. eCollection 2019"
COMMENT     Sequence Label: pcDNA3.1-Hyg-mEGFP
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(72..74,75..77,78..785)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLKE
                     RVTAAGITLGMDELYK"
     primer_bind     complement(114..135)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(375..394)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(863..880)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    869..1093
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cttcaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacgggccct
       61 ctagactcga gatggtgagc aagggcgagc tgttcaccgg ggtggtgccc atcctggtcg
      121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      241 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      541 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      661 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      721 acatggtcct gaaggagcgc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      781 acaaggatat ctgcagaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc
      841 ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      901 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      961 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1021 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1081 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     1141 gcgcc
//
