LOCUS       sequence-371977-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses biosensor for cGMP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Takizawa M, Osuga Y, Ishida R, Mita M, Harada K, Ueda H, Kitaguchi 
            T, Tsuboi T
  TITLE     Development of a red fluorescent protein-based cGMP indicator 
            applicable for live-cell imaging.
  JOURNAL   Commun Biol. 2022 Sep 5;5(1):833. doi: 10.1038/s42003-022-03790-2.
  PUBMED    36064581
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-5"; volume: "5(1):833. doi"; pages: " 
            10.1038/s42003-022-03790-2"
COMMENT     Sequence Label: Red cGull
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     417..436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgctggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
       61 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      121 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
      181 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacattaag
      241 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag
      301 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag
      361 gacggcgtat tcatctacaa ggtgaagctg cgcggcacca acttcccccc cgacggcccc
      421 gtaatgcaga agaagaccat gggctgggag gctctcagtt tttcgctgga tcttgggttg
      481 catatccatg gactcatctc tgcggatcgc tattccctgt tccttgtctg tgaggacagc
      541 tctaaagaca aattcctcat cagccgcctc tttgatgttg ctgaaggttc aacattggag
      601 gaagcctcaa ataactgtat ccgtttagag tggaacaaag gcattgtggg acatgtggca
      661 gcttttggcg agcccttgaa catcaaagat gcctatgagg acccccggtt caatgcagaa
      721 gttgaccaaa ttacaggcta caagacacaa agtatccttt gtatgccaat taagaaccac
      781 agggaagagg ttgttggtgt agctcaggcc atcaacaaga aatcaggaaa tggtgggacc
      841 ttcactgaaa aagatgaaaa ggactttgcg gcctaccttg atcggttaaa gaaggagttg
      901 caggaattcg tttccgagcg gatgtacccc gaggacggcg ccctgaagag cgagatcaag
      961 aaggggctga ggctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag
     1021 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcgtg
     1081 tcccacaacg aggactacac catcgtggaa cagtgcgaac gcgccgaggg ccgccactcc
     1141 accggcggca tggacgagct gtacaagtaa
//
