LOCUS       sequence-371995-       13304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyclin inducible expression of ctrl IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Bonfim-Melo A, Noordstra I, Gupta S, Chan AH, Jones MJK, Schroder K,
            Yap AS
  TITLE     Rapid lamellipodial responses by neighbor cells drive epithelial 
            sealing in response to pyroptotic cell death.
  JOURNAL   Cell Rep. 2022 Feb 1;38(5):110316. doi: 
            10.1016/j.celrep.2022.110316.
  PUBMED    35108534
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-02-1"; volume: "38(5):110316. doi"; pages: " 
            10.1016/j.celrep.2022.110316"
COMMENT     Sequence Label: pTripz Ctrl-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..13304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2078..2195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2260..2634)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2631..2640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2644..2662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2654..2701)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2737..2755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2772..2790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2808..2826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2844..2862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2879..2897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2915..2933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2945..2983
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2980..3004
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3022..3048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3087..3661,3662..3664,3665..3673)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3680..4390
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3825..3843)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4093..4112
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           4412..4505
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4531..4643
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(4738..4756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4770..5280
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5199..5217
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(5293..5326)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5333..6040
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6041..6074)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6097..6669
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6672..7274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7171..7191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7290..7878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7343..7363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7761..7772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8085..8318
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8419..8436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8425..8649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8695..9123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8782..8801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8992..9013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9132..9152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9137..9466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9317..9452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9379..9398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10674..10795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10844..10860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10844..10860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10857..10879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10868..10884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10892..10898,10899..10916,10917..10922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10937..10958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11075..11092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11246..11834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11326..11345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12005..12796,12797..12865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12628..12647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12866..12970)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13018..13152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     2041 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2101 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2161 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2221 atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct cctcggccac
     2281 gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc
     2341 gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga
     2401 ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg
     2461 caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc
     2521 gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc
     2581 tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggtggc
     2641 cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
     2701 cacgtgctgc aggtccgagg ttctagacga gtttactccc tatcagtgat agagaacgat
     2761 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2821 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     2881 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2941 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     3001 gatcgcaccg gtgccaccat gtacccatac gatgtgccag attacgctgg taccggatcc
     3061 gtcgacctcg aggaattcgc ggccgccccc tctccctccc ccccccctaa cgttactggc
     3121 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3181 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3241 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3301 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3361 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3421 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3481 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3541 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa
     3601 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3661 tatggccaca accgaattca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     3721 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     3781 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3841 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     3901 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     3961 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     4021 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     4081 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     4141 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     4201 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     4261 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     4321 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     4381 gtacaagtga gtttaaacat cgatagtttg tttgaatgag gcttcagtac tttacagaat
     4441 cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga
     4501 aggctcgagg taaccggatc ctgatcagaa ttcaaggggc tactttagga gcaattatct
     4561 tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa
     4621 aatggtataa attaaatcac ttttttcaat tggaagacta atgcggccgg ccattactcc
     4681 gtctcgtgtc ttgttgcata tgtctgctgg tttgtttgat gttgtttgcg ggcgggccct
     4741 atagtgagtc gtattaccta ggacgcgtgg gggttggggt tgcgcctttt ccaaggcagc
     4801 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     4861 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     4921 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     4981 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     5041 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     5101 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     5161 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     5221 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     5281 gctagcggat ccataacttc gtatagtata cattatacga agttatgcca ccatgtctag
     5341 gctggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     5401 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5461 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     5521 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     5581 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     5641 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     5701 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     5761 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     5821 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     5881 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5941 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     6001 tgcccttgac gattttgacc ttgacatgct ccccgggtaa ataacttcgt atagtataca
     6061 ttatacgaag ttatggatcc gcggccgcaa attccgcccc tctccctccc ccccccctaa
     6121 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     6181 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     6241 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     6301 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     6361 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6421 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6481 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6541 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6601 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6661 acgataatac catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6721 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6781 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6841 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6901 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg
     6961 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7021 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7081 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg
     7141 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7201 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7261 agcccggtgc ctgagttcgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa
     7321 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7381 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7441 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7501 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7561 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7621 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7681 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7741 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7801 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7861 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc
     7921 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     7981 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     8041 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca
     8101 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8161 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8221 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8281 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8341 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     8401 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     8461 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     8521 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     8581 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8641 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     9001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9121 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     9421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     9481 ttgtatatcc attttcggat ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac
     9541 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     9601 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     9661 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     9721 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttaattcagc gagagcctga
     9781 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     9841 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     9901 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     9961 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
    10021 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
    10081 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
    10141 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
    10201 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
    10261 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
    10321 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
    10381 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
    10441 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
    10501 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgtg ctacgagatt
    10561 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    10621 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
    10681 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10741 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10801 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
    10861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10921 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10981 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    11041 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    11101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    11161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    11281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    11341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    11401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    11461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    11521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    11581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11881 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11941 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    12001 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    12061 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    12121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    12181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    12241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    12301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    12361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    12421 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    12481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    12541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    12601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    12661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    12721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    12781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    12841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    12901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12961 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    13021 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    13081 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    13141 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    13201 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    13261 gaattatttt cattttaaag aaattgtatt tgttaaatat gtac
//
