LOCUS       sequence-372006-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA mcherry
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..271
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     23..43
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     194..213
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        293..368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        518..821
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1076..2092
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2290..2309
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2310..2319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2316..3026
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(2461..2479)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2729..2748
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3050..3638
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3103..3123)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3521..3532)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3845..4478
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4607..4623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4607..4623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4620..4642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4631..4647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4655..4661,4662..4679,4680..4685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4700..4721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4838..4855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5009..5597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5089..5108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5768..6559,6560..6628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6391..6410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6629..6733)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6781..6915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6818..6837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6872..6891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7083..7716
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7763..7888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8385..8618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8803..8847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9110..9225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat
       61 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
      121 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      181 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      241 tttatatatc ttgtggaaag gacgaaacac cggagacgat taatgcgtct cggttttaga
      301 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      361 gtcggtgctt tttttgaatt ctcgacctcg agacaaatgg cagtattcgt cattagttca
      421 tagcccatat atggagttcc gcgttwywtw nnamttmycg acmtcgagac aaatggcagt
      481 attcgtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      541 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      601 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      661 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      721 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      781 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
      841 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
      901 tttaattatt ttgtgcagcg atgggggcgg gggggggggg sgsggggcgg ggcggggcgg
      961 ggcgrggsgg rgrggkgsgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     1021 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     1081 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     1141 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     1201 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     1261 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     1321 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1381 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1441 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1501 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     1561 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     1621 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1681 gccycgggcc ggggrgggct cgggggrggg gcgcggcggc ccccggagcg ccggcggctg
     1741 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1801 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1861 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1921 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1981 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     2041 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     2101 cracgkgcyk kygwgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2161 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2221 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2281 agctcctggg caacgtgctg gttattgtgg ccaccatggt gagcaagggc gaagaagata
     2341 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2401 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2461 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2521 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2581 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2641 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2701 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2761 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2821 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2881 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     2941 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccac cactccaccg
     3001 gcggcatgga cgagctgtac aagtaatgta caagtaaagc gtctggaaca atcaacctct
     3061 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3121 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3181 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3241 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3301 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3361 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3421 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     3481 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3541 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3601 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     3661 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     3721 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     3781 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     3841 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     3901 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     3961 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     4021 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     4081 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     4141 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     4201 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     4261 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     4321 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4381 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4441 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4501 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     4561 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     6781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6901 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     6961 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     7021 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     7081 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     7141 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     7201 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     7261 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     7321 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     7381 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     7441 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     7501 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     7561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7741 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7801 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7861 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7921 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7981 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8041 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8101 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8161 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8221 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     8281 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     8341 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     8401 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     8461 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     8521 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     8581 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     8641 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     8701 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     8761 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     8821 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     8881 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     8941 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9001 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     9061 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa
     9121 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9181 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9241 ggacagcaga gatccagttt atcattagtg aacggatctc gacggtatcg atcacgagac
     9301 tagcc
//
