LOCUS       sequence-372012-       10443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human FLT4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_FLT4
FEATURES             Location/Qualifiers
     source          1..10443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(192..780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(272..291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(951..1742,1743..1811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1574..1593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1812..1916)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1991..2010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2182..2561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2562..2765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2716..2736
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2767..2785
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2788..2806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2816..2840
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2983..3007
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7127..7151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7198..7221
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7258..7281
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7312..7338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7312..7338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7339..7686,7687..7689,7690..8298)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8342..8359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8348..8572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8705..8724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8856..8903
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8911..9933
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8911..9933
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10063..10184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10100..10119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10154..10173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10233..10249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10233..10249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10246..10268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10257..10273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10281..10287,10288..10305,10306..10311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10326..10347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
       61 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1921 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1981 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     2041 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     2101 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     2161 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     2221 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2281 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2341 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2401 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2461 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2521 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2581 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2641 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2701 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2761 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
     2821 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2881 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
     2941 tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     3001 gcagaagggc cgtcaaggcc caccatgcag cggggcgccg cgctgtgcct gcgactgtgg
     3061 ctctgcctgg gactcctgga cggcctggtg agtggctact ccatgacccc cccgaccttg
     3121 aacatcacgg aggagtcaca cgtcatcgac accggtgaca gcctgtccat ctcctgcagg
     3181 ggacagcacc ccctcgagtg ggcttggcca ggagctcagg aggcgccagc caccggagac
     3241 aaggacagcg aggacacggg ggtggtgcga gactgcgagg gcacagacgc caggccctac
     3301 tgcaaggtgt tgctgctgca cgaggtacat gccaacgaca caggcagcta cgtctgctac
     3361 tacaagtaca tcaaggcacg catcgagggc accacggccg ccagctccta cgtgttcgtg
     3421 agagactttg agcagccatt catcaacaag cctgacacgc tcttggtcaa caggaaggac
     3481 gccatgtggg tgccctgtct ggtgtccatc cccggcctca atgtcacgct gcgctcgcaa
     3541 agctcggtgc tgtggccaga cgggcaggag gtggtgtggg atgaccggcg gggcatgctc
     3601 gtgtccacgc cactgctgca cgatgccctg tacctgcagt gcgagaccac ctggggagac
     3661 caggacttcc tttccaaccc cttcctggtg cacatcacag gcaacgagct ctatgacatc
     3721 cagctgttgc ccaggaagtc gctggagctg ctggtagggg agaagctggt cctgaactgc
     3781 accgtgtggg ctgagtttaa ctcaggtgtc acctttgact gggactaccc agggaagcag
     3841 gcagagcggg gtaagtgggt gcccgagcga cgctcccagc agacccacac agaactctcc
     3901 agcatcctga ccatccacaa cgtcagccag cacgacctgg gctcgtatgt gtgcaaggcc
     3961 aacaacggca tccagcgatt tcgggagagc accgaggtca ttgtgcatga aaatcccttc
     4021 atcagcgtcg agtggctcaa aggacccatc ctggaggcca cggcaggaga cgagctggtg
     4081 aagctgcccg tgaagctggc agcgtacccc ccgcccgagt tccagtggta caaggatgga
     4141 aaggcactgt ccgggcgcca cagtccacat gccctggtgc tcaaggaggt gacagaggcc
     4201 agcacaggca cctacaccct cgccctgtgg aactccgctg ctggcctgag gcgcaacatc
     4261 agcctggagc tggtggtgaa tgtgcccccc cagatacatg agaaggaggc ctcctccccc
     4321 agcatctact cgcgtcacag ccgccaggcc ctcacctgca cggcctacgg ggtgcccctg
     4381 cctctcagca tccagtggca ctggcggccc tggacaccct gcaagatgtt tgcccagcgt
     4441 agtctccggc ggcggcagca gcaagacctc atgccacagt gccgtgactg gagggcggtg
     4501 accacgcagg atgccgtgaa ccccatcgag agcctggaca cctggaccga gtttgtggag
     4561 ggaaagaata agactgtgag caagctggtg atccagaatg ccaacgtgtc tgccatgtac
     4621 aagtgtgtgg tctccaacaa ggtgggccag gatgagcggc tcatctactt ctatgtgacc
     4681 accatccccg acggcttcac catcgaatcc aagccatccg aggagctact agagggccag
     4741 ccggtgctcc tgagctgcca agccgacagc tacaagtacg agcatctgcg ctggtaccgc
     4801 ctcaacctgt ccacgctgca cgatgcgcac gggaacccgc ttctgctcga ctgcaagaac
     4861 gtgcatctgt tcgccacccc tctggccgcc agcctggagg aggtggcacc tggggcgcgc
     4921 cacgccacgc tcagcctgag tatcccccgc gtcgcgcccg agcacgaggg ccactatgtg
     4981 tgcgaagtgc aagaccggcg cagccatgac aagcactgcc acaagaagta cctgtcggtg
     5041 caggccctgg aagcccctcg gctcacgcag aacttgaccg acctcctggt gaacgtgagc
     5101 gactcgctgg agatgcagtg cttggtggcc ggagcgcacg cgcccagcat cgtgtggtac
     5161 aaagacgaga ggctgctgga ggaaaagtct ggagtcgact tggcggactc caaccagaag
     5221 ctgagcatcc agcgcgtgcg cgaggaggat gcgggacgct atctgtgcag cgtgtgcaac
     5281 gccaagggct gcgtcaactc ctccgccagc gtggccgtgg aaggctccga ggataagggc
     5341 agcatggaga tcgtgatcct tgtcggtacc ggcgtcatcg ctgtcttctt ctgggtcctc
     5401 ctcctcctca tcttctgtaa catgaggagg ccggcccacg cagacatcaa gacgggctac
     5461 ctgtccatca tcatggaccc cggggaggtg cctctggagg agcaatgcga atacctgtcc
     5521 tacgatgcca gccagtggga attcccccga gagcggctgc acctggggag agtgctcggc
     5581 tacggcgcct tcgggaaggt ggtggaagcc tccgctttcg gcatccacaa gggcagcagc
     5641 tgtgacaccg tggccgtgaa aatgctgaaa gagggcgcca cggccagcga gcaccgcgcg
     5701 ctgatgtcgg agctcaagat cctcattcac atcggcaacc acctcaacgt ggtcaacctc
     5761 ctcggggcgt gcaccaagcc gcagggcccc ctcatggtga tcgtggagtt ctgcaagtac
     5821 ggcaacctct ccaacttcct gcgcgccaag cgggacgcct tcagcccctg cgcggagaag
     5881 tctcccgagc agcgcggacg cttccgcgcc atggtggagc tcgccaggct ggatcggagg
     5941 cggccgggga gcagcgacag ggtcctcttc gcgcggttct cgaagaccga gggcggagcg
     6001 aggcgggctt ctccagacca agaagctgag gacctgtggc tgagcccgct gaccatggaa
     6061 gatcttgtct gctacagctt ccaggtggcc agagggatgg agttcctggc ttcccgaaag
     6121 tgcatccaca gagacctggc tgctcggaac attctgctgt cggaaagcga cgtggtgaag
     6181 atctgtgact ttggccttgc ccgggacatc tacaaagacc ccgactacgt ccgcaagggc
     6241 agtgcccggc tgcccctgaa gtggatggcc cctgaaagca tcttcgacaa ggtgtacacc
     6301 acgcagagtg acgtgtggtc ctttggggtg cttctctggg agatcttctc tctgggggcc
     6361 tccccgtacc ctggggtgca gatcaatgag gagttctgcc agcggctgag agacggcaca
     6421 aggatgaggg ccccggagct ggccactccc gccatacgcc gcatcatgct gaactgctgg
     6481 tccggagacc ccaaggcgag acctgcattc tcggagctgg tggagatcct gggggacctg
     6541 ctccagggca ggggcctgca agaggaagag gaggtctgca tggccccgcg cagctctcag
     6601 agctcagaag agggcagctt ctcgcaggtg tccaccatgg ccctacacat cgcccaggct
     6661 gacgctgagg acagcccgcc aagcctgcag cgccacagcc tggccgccag gtattacaac
     6721 tgggtgtcct ttcccgggtg cctggccaga ggggctgaga cccgtggttc ctccaggatg
     6781 aagacatttg aggaattccc catgacccca acgacctaca aaggctctgt ggacaaccag
     6841 acagacagtg ggatggtgct ggcctcggag gagtttgagc agatagagag caggcataga
     6901 caagaaagcg gcttcagctg taaaggacct ggccagaatg tggctgtgac cagggcacac
     6961 cctgactccc aagggaggcg gcggcggcct gagcgggggg cccgaggagg ccaggtgttt
     7021 tacaacagcg agtatgggga gctgtcggag ccaagcgagg aggaccactg ctccccgtct
     7081 gcccgcgtga ctttcttcac agacaacagc tacttgggcc tcatgggccc agctttcttg
     7141 tacaaagtgg ttgatatcaa ggccgccgac atcaccagcc tgtacaagaa agtggggtgg
     7201 agccatcccc agttcgagaa gggcggagga tctggcggcg gaagcggcgg aggaagctgg
     7261 tcccaccctc agtttgagaa aggcaccgag ctgggcagca ccatggccag ctacccctac
     7321 gacgtgcccg actacgccaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc
     7381 aacggcgagt tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc
     7441 atcaacaagc acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc
     7501 aagggctaca gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag
     7561 ctggatggcg gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc
     7621 gaccggatcg gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc
     7681 agaggcggca ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg
     7741 ttctggagac tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc
     7801 gtgatggccg aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac
     7861 gacctgtatc tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca
     7921 ggcgacgccg cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa
     7981 gagagcgtgg tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg
     8041 aacaccctgg ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag
     8101 gaaggcctgg ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc
     8161 gtgaaactga tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag
     8221 ggggccctgc tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc
     8281 ctgcggagcg ccgagaagtg atgactcgag tctagagggc ccgtttaaac ccgctgatca
     8341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8581 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8821 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat
     8881 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc
     8941 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     9001 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     9061 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     9121 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     9181 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     9241 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     9301 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     9361 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     9421 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     9481 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     9541 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     9601 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     9661 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     9721 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     9781 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     9841 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     9901 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc
     9961 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10021 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    10081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    10201 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    10261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    10321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    10381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10441 cgt
//
