LOCUS       sequence-372013-       13392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human ROS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_ROS1
FEATURES             Location/Qualifiers
     source          1..13392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1995..2014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2186..2565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2566..2769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2771..2789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2792..2810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2820..2844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2987..3011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10080..10104)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10151..10174
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10211..10234
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10265..10291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10265..10291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(10292..10639,10640..10642,10643..11251)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(11295..11312)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11301..11525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11658..11677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    11809..11856
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             11864..12886
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             11864..12886
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    13016..13137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13053..13072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13107..13126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13186..13202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13186..13202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13199..13221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13210..13226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13234..13240,13241..13258,13259..13264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13279..13300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     1981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     2041 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     2101 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     2161 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     2221 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2281 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2341 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2401 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2461 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2521 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2581 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2641 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2701 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2761 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     2821 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2881 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
     2941 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     3001 aaaagcagaa gggccgtcaa ggcccaccat gaagaacatt tactgtctta ttccgaagct
     3061 tgtcaatttt gcaactcttg gctgcctatg gatttctgtg gtgcagtgta cagttttaaa
     3121 tagctgccta aagtcgtgtg taactaatct gggccagcag cttgaccttg gcacaccaca
     3181 taatctgagt gaaccgtgta tccaaggatg tcacttttgg aactctgtag atcagaaaaa
     3241 ctgtgcttta aagtgtcggg agtcgtgtga ggttggctgt agcagcgcgg aaggtgcata
     3301 tgaagaggaa gtactggaaa atgcagacct accaactgct ccctttgctt cttccattgg
     3361 aagccacaat atgacattac gatggaaatc tgcaaacttc tctggagtaa aatacatcat
     3421 tcagtggaaa tatgcacaac ttctgggaag ctggacttat actaagactg tgtccagacc
     3481 gtcctatgtg gtcaagcccc tgcacccctt cactgagtac attttccgag tggtttggat
     3541 cttcacagcg cagctgcagc tctactcccc tccaagtccc agttacagga ctcatcctca
     3601 tggagttcct gaaactgcac ctttgattag gaatattgag agctcaagtc ccgacactgt
     3661 ggaagtcagc tgggatccac ctcaattccc aggtggacct attttgggtt ataacttaag
     3721 gctgatcagc aaaaatcaaa aattagatgc agggacacag agaaccagtt tccagtttta
     3781 ctccacttta ccaaatacta tctacaggtt ttctattgca gcagtaaatg aagttggtga
     3841 gggtccagaa gcagaatcta gtattaccac ttcatcttca gcagttcaac aagaggaaca
     3901 gtggctcttt ttatccagaa aaacttctct aagaaagaga tctttaaaac atttagtaga
     3961 tgaagcacat tgccttcggt tggatgctat ataccataat attacaggaa tatctgttga
     4021 tgtccaccag caaattgttt atttctctga aggaactctc atatgggcga agaaggctgc
     4081 caacatgtct gatgtatctg acctgagaat tttttacaga ggttcaggat taatttcttc
     4141 tatctccata gattggcttt atcaaagaat gtatttcatc atggatgaac tggtatgtgt
     4201 ctgtgattta gagaactgct caaacatcga ggaaattact ccaccctcta ttagtgcacc
     4261 tcaaaaaatt gtggctgatt catacaatgg gtatgtcttt tacctcctga gagatggcat
     4321 ttatagagca gaccttcctg taccatctgg ccggtgtgca gaagctgtgc gtattgtgga
     4381 gagttgcacg ttaaaggact ttgcaatcaa gccacaagcc aagcgaatca tttacttcaa
     4441 tgacactgcc caagtcttca tgtcaacatt tctggatggc tctgcttccc atctcatcct
     4501 acctcgcatc ccctttgctg atgtgaaaag ttttgcttgt gaaaacaatg actttcttgt
     4561 cacagatggc aaggtcattt tccaacagga tgctttgtct tttaatgaat tcatcgtggg
     4621 atgtgacctg agtcacatag aagaatttgg gtttggtaac ttggtcatct ttggctcatc
     4681 ctcccagctg caccctctgc caggccgccc gcaggagctt tcggtgctgt ttggctctca
     4741 ccaggctctt gttcaatgga agcctcctgc ccttgccata ggagccaatg tcatcctgat
     4801 cagtgatatt attgaactct ttgaattagg cccttctgcc tggcagaact ggacctatga
     4861 ggtgaaagta tccacccaag accctcctga agtcactcat attttcttga acataagtgg
     4921 aaccatgctg aatgtacctg agctgcagag tgctatgaaa tacaaggttt ctgtgagagc
     4981 aagttctcca aagaggccag gcccctggtc agagccctca gtgggtacta ccctggtgcc
     5041 agctagtgaa ccaccattta tcatggctgt gaaagaagat gggctttgga gtaaaccatt
     5101 aaatagcttt ggcccaggag agttcttatc ctctgatata ggaaatgtgt cagacatgga
     5161 ttggtataac aacagcctct actacagtga cacgaaaggc gacgtttttg tgtggctgct
     5221 gaatgggacg gatatctcag agaattatca cctacccagc attgcaggag caggggcttt
     5281 agcttttgag tggctgggtc actttctcta ctgggctgga aagacatatg tgatacaaag
     5341 gcagtctgtg ttgacgggac acacagacat tgttacccac gtgaagctat tggtgaatga
     5401 catggtggtg gattcagttg gtggatatct ctactggacc acactctatt cagtggaaag
     5461 caccagacta aatggggaaa gttcccttgt actacagaca cagccttggt tttctgggaa
     5521 aaaggtaatt gctctaactt tagacctcag tgatgggctc ctgtattggt tggttcaaga
     5581 cagtcaatgt attcacctgt acacagctgt tcttcgggga cagagcactg gggataccac
     5641 catcacagaa tttgcagcct ggagtacttc tgaaatttcc cagaatgcac tgatgtacta
     5701 tagtggtcgg ctgttctgga tcaatggctt taggattatc acaactcaag aaataggtca
     5761 gaaaaccagt gtctctgttt tggaaccagc cagatttaat cagttcacaa ttattcagac
     5821 atcccttaag cccctgccag ggaacttttc ctttacccct aaggttattc cagattctgt
     5881 tcaagagtct tcatttagga ttgaaggaaa tgcttcaagt tttcaaatcc tgtggaatgg
     5941 tccccctgcg gtagactggg gtgtagtttt ctacagtgta gaatttagtg ctcattctaa
     6001 gttcttggct agtgaacaac actctttacc tgtatttact gtggaaggac tggaacctta
     6061 tgccttattt aatctttctg tcactcctta tacctactgg ggaaagggcc ccaaaacatc
     6121 tctgtcactt cgagcacctg aaacagttcc atcagcacca gagaacccca gaatatttat
     6181 attaccaagt ggaaaatgct gcaacaagaa tgaagttgtg gtggaattta ggtggaacaa
     6241 acctaagcat gaaaatgggg tgttaacaaa atttgaaatt ttctacaata tatccaatca
     6301 aagtattaca aacaaaacat gtgaagactg gattgctgtc aatgtcactc cctcagtgat
     6361 gtcttttcaa cttgaaggca tgagtcccag atgctttatt gccttccagg ttagggcctt
     6421 tacatctaag gggccaggac catatgctga cgttgtaaag tctacaacat cagaaatcaa
     6481 cccatttcct cacctcataa ctcttcttgg taacaagata gtttttttag atatggatca
     6541 aaatcaagtt gtgtggacgt tttcagcaga aagagttatc agtgccgttt gctacacagc
     6601 tgataatgag atgggatatt atgctgaagg ggactcactc tttcttctgc acttgcacaa
     6661 tcgctctagc tctgagcttt tccaagattc actggttttt gatatcacag ttattacaat
     6721 tgactggatt tcaaggcacc tctactttgc actgaaagaa tcacaaaatg gaatgcaagt
     6781 atttgatgtt gatcttgaac acaaggtgaa atatcccaga gaggtgaaga ttcacaatag
     6841 gaattcaaca ataatttctt tttctgtata tcctctttta agtcgcttgt attggacaga
     6901 agtttccaat tttggctacc agatgttcta ctacagtatt atcagtcaca ccttgcaccg
     6961 aattctgcaa cccacagcta caaaccaaca aaacaaaagg aatcaatgtt cttgtaatgt
     7021 gactgaattt gagttaagtg gagcaatggc tattgatacc tctaacctag agaaaccatt
     7081 gatatacttt gccaaagcac aagagatctg ggcaatggat ctggaaggct gtcagtgttg
     7141 gagagttatc acagtacctg ctatgctcgc aggaaaaacc cttgttagct taactgtgga
     7201 tggagatctt atatactgga tcatcacagc aaaggacagc acacagattt atcaggcaaa
     7261 gaaaggaaat ggggccatcg tttcccaggt gaaggcccta aggagtaggc atatcttggc
     7321 ttacagttca gttatgcagc cttttccaga taaagcgttt ctgtctctag cttcagacac
     7381 tgtggaacca actatactta atgccactaa cactagcctc acaatcagat tacctctggc
     7441 caagacaaac ctcacatggt atggcatcac cagccctact ccaacatacc tggtttatta
     7501 tgcagaagtt aatgacagga aaaacagctc tgacttgaaa tatagaattc tggaatttca
     7561 ggacagtata gctcttattg aagatttaca accattttca acatacatga tacagatagc
     7621 tgtaaaaaat tattattcag atcctttgga acatttacca ccaggaaaag agatttgggg
     7681 aaaaactaaa aatggagtac cagaggcagt gcagctcatt aatacaactg tgcggtcaga
     7741 caccagcctc attatatctt ggagagaatc tcacaagcca aatggaccta aagaatcagt
     7801 ccgttatcag ttggcaatct cacacctggc cctaattcct gaaactcctc taagacaaag
     7861 tgaatttcca aatggaaggc tcactctcct tgttactaga ctgtctggtg gaaatattta
     7921 tgtgttaaag gttcttgcct gccactctga ggaaatgtgg tgtacagaga gtcatcctgt
     7981 cactgtggaa atgtttaaca caccagagaa accttattcc ttggttccag agaacactag
     8041 tttgcaattt aattggaagg ctccattgaa tgttaacctc atcagatttt gggttgagct
     8101 acagaagtgg aaatacaatg agttttacca tgttaaaact tcatgcagcc aaggtcctgc
     8161 ttatgtctgt aatatcacaa atctacaacc ttatacttca tataatgtca gagtagtggt
     8221 ggtttataag acgggagaaa atagcacctc acttccagaa agctttaaga caaaagctgg
     8281 agtcccaaat aaaccaggca ttcccaaatt actagaaggg agtaaaaatt caatacagtg
     8341 ggagaaagct gaagataatg gatgtagaat tacatactat atccttgaga taagaaagag
     8401 cacttcaaat aatttacaga accagaattt aaggtggaag atgacattta atggatcctg
     8461 cagtagtgtt tgcacatgga agtccaaaaa cctgaaagga atatttcagt tcagagtagt
     8521 agctgcaaat aatctagggt ttggtgaata tagtggaatc agtgagaata ttatattagt
     8581 tggagatgat ttttggatac cagaaacaag tttcatactt actattatag ttggaatatt
     8641 tctggttgtt acaatcccac tgacctttgt ctggcataga agattaaaga atcaaaaaag
     8701 tgccaaggaa ggggtgacag tgcttataaa cgaagacaaa gagttggctg agctgcgagg
     8761 tctggcagcc ggagtaggcc tggctaatgc ctgctatgca atacatactc ttccaaccca
     8821 agaggagatt gaaaatcttc ctgccttccc tcgggaaaaa ctgactctgc gtctcttgct
     8881 gggaagtgga gcctttggag aagtgtatga aggaacagca gtggacatct taggagttgg
     8941 aagtggagaa atcaaagtag cagtgaagac tttgaagaag ggttccacag accaggagaa
     9001 gattgaattc ctgaaggagg cacatctgat gagcaaattt aatcatccca acattctgaa
     9061 gcagcttgga gtttgtctgc tgaatgaacc ccaatacatt atcctggaac tgatggaggg
     9121 aggagacctt cttacttatt tgcgtaaagc ccggatggca acgttttatg gtcctttact
     9181 caccttggtt gaccttgtag acctgtgtgt agatatttca aaaggctgtg tctacttgga
     9241 acggatgcat ttcattcaca gggatctggc agctagaaat tgccttgttt ccgtgaaaga
     9301 ctataccagt ccacggatag tgaagattgg agactttgga ctcgccagag acatctataa
     9361 aaatgattac tatagaaaga gaggggaagg cctgctccca gttcggtgga tggctccaga
     9421 aagtttgatg gatggaatct tcactactca atctgatgta tggtcttttg gaattctgat
     9481 ttgggagatt ttaactcttg gtcatcagcc ttatccagct cattccaacc ttgatgtgtt
     9541 aaactatgtg caaacaggag ggagactgga gccaccaaga aattgtcctg atgatctgtg
     9601 gaatttaatg acccagtgct gggctcaaga acccgaccaa agacctactt ttcatagaat
     9661 tcaggaccaa cttcagttat tcagaaattt tttcttaaat agcatttata agtccagaga
     9721 tgaagcaaac aacagtggag tcataaatga aagctttgaa ggtgaagatg gcgatgtgat
     9781 ttgtttgaat tcagatgaca ttatgccagt tgctttaatg gaaacgaaga accgagaagg
     9841 gttaaactat atggtacttg ctacagaatg tggccaaggt gaagaaaagt ctgagggtcc
     9901 tctaggctcc caggaatctg aatcttgtgg tctgaggaaa gaagagaagg aaccacatgc
     9961 agacaaagat ttctgccaag aaaaacaagt ggcttactgc ccttctggca agcctgaagg
    10021 cctgaactat gcctgtctca ctcacagtgg atatggagat gggtctgatg gcctcatggg
    10081 cccagctttc ttgtacaaag tggttgatat caaggccgcc gacatcacca gcctgtacaa
    10141 gaaagtgggg tggagccatc cccagttcga gaagggcgga ggatctggcg gcggaagcgg
    10201 cggaggaagc tggtcccacc ctcagtttga gaaaggcacc gagctgggca gcaccatggc
    10261 cagctacccc tacgacgtgc ccgactacgc caaggacaac accgtgcccc tgaagctgat
    10321 cgccctgctg gccaacggcg agttccactc tggcgagcag ctgggcgaga cactgggcat
    10381 gagcagagcc gccatcaaca agcacatcca gaccctgcgg gactggggcg tggacgtgtt
    10441 cacagtgccc ggcaagggct acagcctgcc cgagcccatc cagctgctga acgccaagca
    10501 gatcctgggc cagctggatg gcggatctgt ggccgtgctg cccgtgatcg acagcaccaa
    10561 ccagtatctg ctcgaccgga tcggcgagct gaagtccggc gacgcctgta tcgccgagta
    10621 ccagcaggcc ggcagaggcg gcaggggcag aaagtggttc agccccttcg gcgccaacct
    10681 gtacctgagc atgttctgga gactggaaca gggcccagcc gccgctattg gcctgagcct
    10741 ggtcatcggc atcgtgatgg ccgaggtgct gcggaagctg ggcgccgaca aagtgcgcgt
    10801 gaagtggccc aacgacctgt atctgcagga ccggaagctg gccggcatcc tggtggaact
    10861 gaccggcaaa acaggcgacg ccgcccagat cgtgatcggc gctggcatca acatggccat
    10921 gcggcgggtg gaagagagcg tggtcaacca gggctggatc accctgcagg aagccggcat
    10981 caacctggac cggaacaccc tggccgccat gctgatcaga gagctgagag ccgccctgga
    11041 actgttcgag caggaaggcc tggcccccta cctgagcaga tgggagaagc tggacaactt
    11101 catcaaccgg cccgtgaaac tgatcatcgg cgacaaagag atcttcggca tcagccgggg
    11161 catcgacaag cagggggccc tgctcctgga acaggacggc atcatcaagc cctggatggg
    11221 cggcgagatc agcctgcgga gcgccgagaa gtgatgactc gagtctagag ggcccgttta
    11281 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    11341 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    11401 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    11461 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    11521 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
    11581 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    11641 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    11701 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    11761 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
    11821 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
    11881 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
    11941 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
    12001 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
    12061 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
    12121 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
    12181 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
    12241 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
    12301 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
    12361 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
    12421 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
    12481 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
    12541 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
    12601 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
    12661 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
    12721 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
    12781 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
    12841 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
    12901 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    12961 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
    13021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    13081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    13141 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
    13201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    13261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    13321 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    13381 gagaggcggt tt
//
