LOCUS       sequence-372019-        9540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human RET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_RET
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(943..1734,1735..1803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1566..1585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1804..1908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1983..2002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2174..2553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2554..2757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2708..2728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2759..2777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2780..2798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2808..2832
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2975..2999
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6216..6240)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6287..6310
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6347..6370
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6401..6427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6401..6427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6428..6775,6776..6778,6779..7387)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7431..7448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7437..7661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7794..7813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7945..7992
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8000..9022
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8000..9022
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9152..9273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9189..9208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9243..9262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9322..9338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9322..9338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9335..9357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9346..9362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9370..9376,9377..9394,9395..9400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9415..9436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1921 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     1981 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     2041 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     2101 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     2161 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     2221 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2281 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2341 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2401 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2461 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2521 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2581 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2641 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2701 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     2761 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     2821 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2881 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc
     2941 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagttggcac
     3001 catggcgaag gcgacgtccg gtgccgcggg gctgcgtctg ctgttgctgc tgctgctgcc
     3061 gctgctaggc aaagtggcat tgggcctcta cttctcgagg gatgcttact gggagaagct
     3121 gtatgtggac caggcggccg gcacgccctt gctgtacgtc catgccctgc gggacgcccc
     3181 tgaggaggtg cccagcttcc gcctgggcca gcatctctac ggcacgtacc gcacacggct
     3241 gcatgagaac aactggatct gcatccagga ggacaccggc ctcctctacc ttaaccggag
     3301 cctggaccat agctcctggg agaagctcag tgtccgcaac cgcggctttc ccctgctcac
     3361 cgtctacctc aaggtcttcc tgtcacccac atcccttcgt gagggcgagt gccagtggcc
     3421 aggctgtgcc cgcgtatact tctccttctt caacacctcc tttccagcct gcagctccct
     3481 caagccccgg gagctctgct tcccagagac aaggccctcc ttccgcattc gggagaaccg
     3541 acccccaggc accttccacc agttccgcct gctgcctgtg cagttcttgt gccccaacat
     3601 cagcgtggcc tacaggctcc tggagggtga gggtctgccc ttccgctgcg ccccggacag
     3661 cctggaggtg agcacgcgct gggccctgga ccgcgagcag cgggagaagt acgagctggt
     3721 ggccgtgtgc accgtgcacg ccggcgcgcg cgaggaggtg gtgatggtgc ccttcccggt
     3781 gaccgtgtac gacgaggacg actcggcgcc caccttcccc gcgggcgtcg acaccgccag
     3841 cgccgtggtg gagttcaagc ggaaggagga caccgtggtg gccacgctgc gtgtcttcga
     3901 tgcagacgtg gtacctgcat caggggagct ggtgaggcgg tacacaagca cgctgctccc
     3961 cggggacacc tgggcccagc agaccttccg ggtggaacac tggcccaacg agacctcggt
     4021 ccaggccaac ggcagcttcg tgcgggcgac cgtacatgac tataggctgg ttctcaaccg
     4081 gaacctctcc atctcggaga accgcaccat gcagctggcg gtgctggtca atgactcaga
     4141 cttccagggc ccaggagcgg gcgtcctctt gctccacttc aacgtgtcgg tgctgccggt
     4201 cagcctgcac ctgcccagta cctactccct ctccgtgagc aggagggctc gccgatttgc
     4261 ccagatcggg aaagtctgtg tggaaaactg ccaggcattc agtggcatca acgtccagta
     4321 caagctgcat tcctctggtg ccaactgcag cacgctaggg gtggtcacct cagccgagga
     4381 cacctcgggg atcctgtttg tgaatgacac caaggccctg cggcggccca agtgtgccga
     4441 acttcactac atggtggtgg ccaccgacca gcagacctct aggcaggccc aggcccagct
     4501 gcttgtaaca gtggaggggt catatgtggc cgaggaggcg ggctgccccc tgtcctgtgc
     4561 agtcagcaag agacggctgg agtgtgagga gtgtggcggc ctgggctccc caacaggcag
     4621 gtgtgagtgg aggcaaggag atggcaaagg gatcaccagg aacttctcca cctgctctcc
     4681 cagcaccaag acctgccccg acggccactg cgatgttgtg gagacccaag acatcaacat
     4741 ttgccctcag gactgcctcc ggggcagcat tgttggggga cacgagcctg gggagccccg
     4801 ggggattaaa gctggctatg gcacctgcaa ctgcttccct gaggaggaga agtgcttctg
     4861 cgagcccgaa gacatccagg atccactgtg cgacgagctg tgccgcacgg tgatcgcagc
     4921 cgctgtcctc ttctccttca tcgtctcggt gctgctgtct gccttctgca tccactgcta
     4981 ccacaagttt gcccacaagc cacccatctc ctcagctgag atgaccttcc ggaggcccgc
     5041 ccaggccttc ccggtcagct actcctcttc cagtgcccgc cggccctcgc tggactccat
     5101 ggagaaccag gtctccgtgg atgccttcaa gatcctggag gatccaaagt gggaattccc
     5161 tcggaagaac ttggttcttg gaaaaactct aggagaaggc gaatttggaa aagtggtcaa
     5221 ggcaacggcc ttccatctga aaggcagagc agggtacacc acggtggccg tgaagatgct
     5281 gaaagagaac gcctccccga gtgagcttcg agacctgctg tcagagttca acgtcctgaa
     5341 gcaggtcaac cacccacatg tcatcaaatt gtatggggcc tgcagccagg atggcccgct
     5401 cctcctcatc gtggagtacg ccaaatacgg ctccctgcgg ggcttcctcc gcgagagccg
     5461 caaagtgggg cctggctacc tgggcagtgg aggcagccgc aactccagct ccctggacca
     5521 cccggatgag cgggccctca ccatgggcga cctcatctca tttgcctggc agatctcaca
     5581 ggggatgcag tatctggccg agatgaagct cgttcatcgg gacttggcag ccagaaacat
     5641 cctggtagct gaggggcgga agatgaagat ttcggatttc ggcttgtccc gagatgttta
     5701 tgaagaggat tcgtacgtga agaggagcca gggtcggatt ccagttaaat ggatggcaat
     5761 tgaatccctt tttgatcata tctacaccac gcaaagtgat gtatggtctt ttggtgtcct
     5821 gctgtgggag atcgtgaccc tagggggaaa cccctatcct gggattcctc ctgagcggct
     5881 cttcaacctt ctgaagaccg gccaccggat ggagaggcca gacaactgca gcgaggagat
     5941 gtactgcctg atgctgcaat gctggaagca ggagccggac aaaaggccgg tgtttgcgga
     6001 catcagcaaa gacctggaga agatgatggt taagaggaga gactacttgg accttgcggc
     6061 gtccactcca tctgactccc tgatttatga cgacggcctc tcagaggagg agacaccgct
     6121 ggtggactgt aataatgccc ccctccctcg agccctccct tccacatgga ttgaaaacaa
     6181 actctatggt agaatttccc atgcatttac tagattccca actttcttgt acaaagtggt
     6241 tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga gccatcccca
     6301 gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt cccaccctca
     6361 gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg acgtgcccga
     6421 ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt
     6481 ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca tcaacaagca
     6541 catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca agggctacag
     6601 cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc tggatggcgg
     6661 atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg accggatcgg
     6721 cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca gaggcggcag
     6781 gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggagact
     6841 ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg tgatggccga
     6901 ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct
     6961 gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc
     7021 ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag agagcgtggt
     7081 caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga acaccctggc
     7141 cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg aaggcctggc
     7201 cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg tgaaactgat
     7261 catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg gggccctgct
     7321 cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc
     7381 cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     7441 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7501 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7561 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7621 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7681 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7741 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7801 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7861 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7921 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     7981 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     8041 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     8101 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     8161 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     8221 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     8281 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     8341 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     8401 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     8461 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     8521 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     8581 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     8641 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     8701 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     8761 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     8821 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     8881 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     8941 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     9001 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     9061 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     9121 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     9181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     9301 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
//
