LOCUS       sequence-372032-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP fused to the C-terminus of a WWE domain containing the mutation
            Arg163Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Koczor CA, Haider AJ, Saville KM, Li J, Andrews JF, Beiser AV, Sobol
            RW
  TITLE     Live Cell Detection of Poly(ADP-Ribose) for Use in Genetic and 
            Genotoxic Compound Screens.
  JOURNAL   Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 
            10.3390/cancers14153676.
  PUBMED    35954352
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2022-07-28"; volume: "14(15). pii: 
            cancers14153676. doi"; pages: " 10.3390/cancers14153676"
COMMENT     Sequence Label: pLV-EF1A-LivePAR(R163A)-Hygro
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             246..426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    473..598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1091..1324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1509..1553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1819..1936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1954..1974
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1975..3153
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2206..3144
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3101..3121
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3153..3177
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3178..3187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3460..3462,3463..3465,3466..4179)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3460..3462,3463..3465,3466..4176)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3505..3526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3766..3785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4113..4134
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4180..4204)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4222..4810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4275..4295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4693..4704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4841..5340
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4876..4899)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5273..5292
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5357..6382
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5357..6382
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6454..6687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6759..6880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6796..6815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6850..6869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6920..7055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7075..7094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7076..7094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7104..7121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7104..7120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7113..7135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7262..7717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7349..7368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7559..7580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7743..7847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7848..7916,7917..8708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8066..8085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8879..9467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9368..9387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9621..9638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9755..9776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9791..9796,9797..9814,9815..9821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9829..9845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9834..9856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9853..9869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9853..9869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9888..9908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9890..9908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
       61 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
      121 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
      181 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
      241 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      421 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      481 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      541 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      601 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      661 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      721 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      781 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      841 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      901 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
      961 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1021 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1081 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1141 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1201 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1261 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1321 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1381 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1441 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1501 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1561 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1621 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1681 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     1741 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     1801 ctcgacggta tcgctagctt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     1861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     1921 aaaaattcaa aattttacta gtgattatcg gatcaacttt gtatagaaaa gttgggctcc
     1981 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2041 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2101 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2161 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     2221 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     2281 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     2341 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2401 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2461 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2521 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     2581 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     2641 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggtctc
     2701 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     2761 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     2821 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     2881 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     2941 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3001 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3061 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3121 caaagttttt ttcttccatt tcaggtgtcg tgacaagttt gtacaaaaaa gcaggctgcc
     3181 accatgggaa atggtgaata tgcatggtat tatgaaggaa gaaatgggtg gtggcagtac
     3241 gatgagcgca ctagtagaga gctggaagat gctttttcca aaggtaaaaa gaacactgaa
     3301 atgttaattg ctggctttct gtatgtcgct gatcttgaaa acatggttca atataggaga
     3361 aatgaacatg gacgtcgcgc taagattaag cgagatataa tagatatacc aaagaaggga
     3421 gtagctggac ttaggctaga cgggtcgacc ggtagttcaa tggtgagcaa gggcgaggag
     3481 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3541 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3601 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3661 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3721 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3781 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3841 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3901 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3961 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4021 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4081 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4141 gccgggatca ctctcggcat ggacgagctg tacaagtaaa cccagctttc ttgtacaaag
     4201 tggtgataat cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact
     4261 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4321 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4381 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4441 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4501 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4561 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     4621 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4681 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4741 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4801 gcctccccgc atcgggaatt cccgcggttc gaattctacc gggtagggga ggcgcttttc
     4861 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     4921 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     4981 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     5041 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     5101 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     5161 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     5221 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     5281 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     5341 acctcacgtg gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     5401 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     5461 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     5521 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     5581 ggaagtgctt gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc
     5641 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     5701 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     5761 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     5821 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     5881 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     5941 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     6001 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     6061 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     6121 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     6181 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     6241 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     6301 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     6361 tcgtccgagg gcaaaggaat agggtacctt taagaccaat gacttacaag gcagctgtag
     6421 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6481 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6661 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6721 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6781 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6841 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6901 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6961 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7021 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7081 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7141 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7201 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7261 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7381 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7441 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7501 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7561 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7621 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7681 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga
     9301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9661 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9721 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9781 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9841 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     9901 actaaaggga acaaaag
//
