LOCUS       sequence-372035-       11052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split luciferase construct with nLuc fused to C-terminus of WWE 
            domain with Arg163Ala mutation, linked by T2A to cLuc fused to 
            C-terminus of WWE domain, linked by IRES to puromycin resistance 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Koczor CA, Haider AJ, Saville KM, Li J, Andrews JF, Beiser AV, Sobol
            RW
  TITLE     Live Cell Detection of Poly(ADP-Ribose) for Use in Genetic and 
            Genotoxic Compound Screens.
  JOURNAL   Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 
            10.3390/cancers14153676.
  PUBMED    35954352
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2022-07-28"; volume: "14(15). pii: 
            cancers14153676. doi"; pages: " 10.3390/cancers14153676"
COMMENT     Sequence Label: 
            pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        70..296
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             297..477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    524..649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1142..1375
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1560..1604
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1870..1987
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1996..2016
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2017..3195
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2248..3186
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3143..3163
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3195..3219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3220..3229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3535..3561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4810..4833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4843..4896
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5200..5229
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5692..5715
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5719..5743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5744..6318,6319..6321,6322..6330)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5911..5928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6138..6157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6331..6930
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6331..6350)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6827..6847
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6931..6951)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6964..7552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7017..7037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7435..7446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7640..7873
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7945..8066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7982..8001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8036..8055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8106..8241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8261..8280)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8262..8280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8290..8307)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8290..8306)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8299..8321)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8448..8903
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8535..8554)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8745..8766
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8929..9033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9034..9102,9103..9894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9252..9271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10065..10653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10554..10573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10807..10824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10941..10962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10977..10982,10983..11000,11001..11007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11015..11031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11020..11042
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(11039..11052,1..3)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(11039..11052,1..3)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
       61 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
      121 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
      181 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
      241 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
      301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      481 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      541 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      601 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      661 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      721 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
      781 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
      841 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
      901 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
      961 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1021 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1081 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1141 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1201 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1261 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1321 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1381 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1441 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1501 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1561 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1621 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1681 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1741 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1801 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     1861 atcgctagct tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1921 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1981 aaattttact agtatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca
     2041 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     2101 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     2161 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     2221 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     2281 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     2341 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     2401 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     2461 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     2521 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     2581 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     2641 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     2701 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc
     2761 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     2821 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     2881 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     2941 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     3001 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3061 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3121 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3181 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatggg aaatggtgaa
     3241 tatgcatggt attatgaagg aagaaatggg tggtggcagt acgatgagcg cactagtaga
     3301 gagctggaag atgctttttc caaaggtaaa aagaacactg aaatgttaat tgctggcttt
     3361 ctgtatgtcg ctgatcttga aaacatggtt caatatagga gaaatgaaca tggacgtcgc
     3421 gctaagatta agcgagatat aatagatata ccaaagaagg gagtagctgg acttaggatc
     3481 tccggacaga tcagctacgc cagccggggc ggaggtagct ctggcggagg tgcctaccca
     3541 tacgatgttc cagattacgc tgcagaagat gccaaaaaca ttaagaaggg cccagcgcca
     3601 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     3661 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     3721 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     3781 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     3841 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     3901 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     3961 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     4021 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     4081 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     4141 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     4201 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     4261 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     4321 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     4381 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     4441 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     4501 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     4561 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     4621 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     4681 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     4741 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     4801 aaggacggcg actacaagga cgacgatgac aagggaagcg gagagggcag gggaagtctt
     4861 ctaacatgcg gggacgtgga ggaaaatccc ggccccatgg gcaacggcga gtatgcatgg
     4921 tattacgaag gcagaaacgg atggtggcaa tatgatgaaa gaacttctcg agaactggaa
     4981 gatgcgttct ctaaagggaa gaagaatact gaaatgttaa tagcgggatt tctttacgtc
     5041 gccgacttag aaaacatggt gcaataccgg cgaaatgagc acggccggag aaggaaaata
     5101 aagagggata ttatagatat ccccaagaaa ggtgtggctg ggttaaggtc ccagatcagc
     5161 tacgccagcc ggggcggagg tagctctggc ggaggtgccg agcagaagct gatcagcgag
     5221 gaagacctgt ccggcagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     5281 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     5341 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     5401 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     5461 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     5521 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     5581 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     5641 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt ggactacaag
     5701 gacgacgatg acaagtgaac ccagctttct tgtacaaagt gggcccctct ccctcccccc
     5761 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5821 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5881 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5941 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6001 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6061 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6121 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6181 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6241 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6301 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     6361 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6421 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6481 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6541 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6601 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6661 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6721 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6781 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6841 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6901 tggtgcatga cccgcaagcc cggtgcctga caactttatt atacatagtt gatcaattcc
     6961 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7321 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7441 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7501 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa
     7561 ttcccgcggt tcgctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7621 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7681 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7861 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7921 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7981 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8041 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8101 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8161 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8221 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8281 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8341 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8401 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8461 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8521 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8581 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8641 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8701 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8761 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8821 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8881 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8941 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9001 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9061 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9121 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9181 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9241 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9301 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9361 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9421 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9481 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9541 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9601 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9661 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9721 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9781 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9841 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9901 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9961 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10021 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10081 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10141 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10201 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg
    10501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10681 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10741 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10801 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10861 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10921 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10981 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    11041 ggaaacagct at
//
