LOCUS       sequence-37204-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Nallamsetty S, Austin BP, Penrose KJ, Waugh DS
  TITLE     Gateway vectors for the production of combinatorially-tagged 
            His6-MBP fusion proteins in the cytoplasm and periplasm of 
            Escherichia coli.
  JOURNAL   Protein Sci. 2005 Dec . 14(12):2964-71.
  PUBMED    16322578
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Dec . 14(12):2964-71."
COMMENT     Sequence Label: pDEST-periHisMBP
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcacgagc acttcaccaa caaggaccat agattatgaa aataaaaaca ggtgcacgca
       61 tcctcgcatt atccgcatta acgacgatga tgttttccgc ctcggctctc gccaaaatcc
      121 atcaccatca ccatcacgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct
      181 ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg
      241 ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact ggcgatggcc
      301 ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct ggcctgttgg
      361 ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc tgggatgccg
      421 tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta tcgctgattt
      481 ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata
      541 aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca
      601 cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg
      661 acattaaaga cgt
//
