LOCUS       sequence-372097-        5759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IL2-Rhotekin GTPase-binding domain-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: IL2-mCherry-rGBD in pmCherryN1
FEATURES             Location/Qualifiers
     source          1..5759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        129..320
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     277..297
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(322..426)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      453..908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(540..559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     750..771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    915..1036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(926..945)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     980..999
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1437..1456)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1706..1724
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1866..1875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3015..3218)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3044..3064)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3219..3522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3697..4285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3777..3796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4614..4661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4686..4705
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4893..5687)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4893..5687)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5004..5023)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5614..5633
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5722..5759,1..320))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5736..5759,1..112)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
       61 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
      121 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      181 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
      241 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
      301 gctggttctt tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg
      361 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      421 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
      481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
      841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
      901 tacaatttac gccttaagat acattgatga gtttggacaa accacaacta gaatgcagtg
      961 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1021 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1081 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     1141 tctagagtcg cggccgcttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
     1201 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
     1261 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
     1321 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
     1381 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
     1441 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
     1501 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
     1561 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
     1621 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     1681 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
     1741 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     1801 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgtccat
     1861 gctcaccatg gtggcgaccg gtgcgcctgt cttctccagc acctgggcct cctttcgccg
     1921 ctgcagctca cccatgtagc tgagaatacg gctgttgcac accagcaggc tcttggtggc
     1981 ttccagagcc tgctctcgct gggagcaggc tgccagcagc ttgcaggccc catccctcat
     2041 ccggatctca tgatctagtt tcctctgcag ctctgtgtcc tccggcgact cgctgaagaa
     2101 actaagtcgg aagcggccgc gtttgaactc catctccagg gcggagcccc tggccactgt
     2161 gatccgactc accggtatct tccaggtgag cccactcagg aggaggacgc tgatcagcag
     2221 gaaaacacag ccggccactg ctacctggta ctctgttgta aatatggacg tctccatggt
     2281 tgcagccatt tctgtctgta tttgaaaatc tgttgttgtg acgaggcagg aagtctcact
     2341 ctcaggacgg ccttcggggc ttgcctgagg cttctcttca cctggaaact gactggtctc
     2401 catttcacct gtgcatatga gctggggctg ggtccacctt gtcttcccgt gggtcatttt
     2461 gcagacgctc tcagcaggac ctctgtgtag agccctgtat ccctggacgc actgataata
     2521 aaccatctgc cccaccacga aatgataaat tctctctgtg gcttcatttt cccatggtgg
     2581 aggttccctg cagtgacctg gaaggctcgc ttggtccact ggctgcattg gactttgcat
     2641 ttctgtggtt ttcctttctt tctgttcttc aggttgaggt gtcacttgtt tcgttgtgtt
     2701 ccgagtggca gagcttgtgc attgacattg gttgtcccag gacgagtggc tagagtttcc
     2761 tgtacagagc atatagagtg acccgctttt tattctgcgg aaacctctct tgcattcaca
     2821 gttcaacatg gttccttcct tgtaggccat ggctttgaat gtggcgtgtg ggatctctgg
     2881 cgggtcatcg tcacagagct ctgcctggca gccaggcacc atgatgaacg tgagcagtcc
     2941 ccacatcagc aggtatgaat ccatctcgag atctgagtcc ggtagcgcta gcggatctga
     3001 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3061 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3121 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3181 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3241 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3301 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3361 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3421 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3481 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3541 ctatgaacta atgaccccgt aattgattac tattaataac taatgcatgg cggtaatacg
     3601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4441 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4501 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4561 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4621 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     4681 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     4741 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4801 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4861 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4921 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4981 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     5041 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5101 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     5161 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     5221 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     5281 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     5341 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5401 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5461 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5521 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5581 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5641 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     5701 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcact
//
