LOCUS       sequence-372098-       11761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 11761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-s ONE-GO
FEATURES             Location/Qualifiers
     source          1..11761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(646..648,649..1359)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(688..709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(949..968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2228..2802,2803..2805,2806..2814)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2395..2412)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2622..2641
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2825..2845
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             2954..3466
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             3476..3529
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5226..5814
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5279..5299)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5697..5708)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6021..6654
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6782..6798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6782..6798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6795..6817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6806..6822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6830..6836,6837..6854,6855..6860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6875..6896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7013..7030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7184..7772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7264..7283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7943..8734,8735..8803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8566..8585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8804..8908)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8956..9090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8993..9012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9047..9066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9258..9891
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9938..10063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10560..10793
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10978..11022
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11285..11400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        11458..11761
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgg gctgcctcgg gaacagtaag
      361 accgaggacc agcgcaacga ggagaaggcg cagcgtgagg ccaacaaaaa gatcgagaag
      421 cagctgcaga aggacaagca ggtctaccgg gccacgcacc gcctgctgct gctgggtgct
      481 ggagaatctg gtaaaagcac cattgtgaag cagatgagga tcctgcatgt taatgggttt
      541 aatggagaca gtgagaaggc aaccaaagtg caggacatca aaaacaacct gaaagaggcg
      601 attgaaacca ttgtggccgc catgagcaac ctgggtggcg gaggggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      781 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
      841 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      901 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      961 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1021 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1081 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     1141 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1261 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtggggg agtgcccccc
     1381 gtggagctgg ccaaccccga gaaccagttc agagtggact acattctgag tgtgatgaac
     1441 gtgcctgact ttgacttccc tcccgaattc tatgagcatg ccaaggctct gtgggaggat
     1501 gaaggagtgc gtgcctgcta cgaacgctcc aacgagtacc agctgattga ctgtgcccag
     1561 tacttcctgg acaagatcga cgtgatcaag caggctgact atgtgccgag cgatcaggac
     1621 ctgcttcgct gccgtgtcct gacttctgga atctttgaga ccaagttcca ggtggacaaa
     1681 gtcaacttcc acatgtttga cgtgggtggc cagcgcgatg aacgccgcaa gtggatccag
     1741 tgcttcaacg atgtgactgc catcatcttc gtggtggcca gcagcagcta caacatggtc
     1801 atccgggagg acaaccagac caaccgcctg caggaggctc tgaacctctt caagagcatc
     1861 tggaacaaca gatggctgcg caccatctct gtgatcctgt tcctcaacaa gcaagatctg
     1921 ctcgctgaga aagtccttgc tgggaaatcg aagattgagg actactttcc agaatttgct
     1981 cgctacacta ctcctgagga tgctactccc gagcccggag aggacccacg cgtgacccgg
     2041 gccaagtact tcattcgaga tgagtttctg aggatcagca ctgccagtgg agatgggcgt
     2101 cactactgct accctcattt cacctgcgct gtggacactg agaacatccg ccgtgtgttc
     2161 aacgactgcc gtgacatcat tcagcgcatg caccttcgtc agtacgagct gctataagcg
     2221 gccgccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2281 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2341 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     2401 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     2461 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     2521 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     2581 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     2641 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     2701 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     2761 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgtacc
     2821 aaagatgggg agtagcaaga gcaagacgtc gaattccgga atcaagcttg gtggatcatc
     2881 aggaggtagc agggagctgg cctggggcat cagcgagtgg ctggaggagt ggggcagcgg
     2941 aggtggtgga tccatggtct tcacactcga agatttcgtt ggggactggc gacagacagc
     3001 cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct
     3061 cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat
     3121 cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga
     3181 aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta
     3241 tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta
     3301 tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg
     3361 caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac
     3421 catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgggat caggagaagg
     3481 tagaggaagt ctactaacat gcggggacgt ggaggaaaat cccggcccaa tggatgaaga
     3541 gcgcgccctc tacatcgtcc gggccggcga ggcgggggct atcgagcggg tcctgaggga
     3601 ttacagcgac aagcataggg ctactttcaa atttgaatca gcagatgaag ataaaagaaa
     3661 gaaactctgt gaaggcatat ttaaggtcct tgtcaaggag gtcccaacga catgccaagt
     3721 gtcctgcctg gaagtactcc gcattctctc cagagacaaa aagatcttag ttcctgtaac
     3781 aactaaggaa aatatgcaaa tactgctgag actagccaag ctccacgagt cagacgattc
     3841 tttggagaag gtgtcagagt tcccagttat tgtggaatca ttgaagtgtc tgtgtaacat
     3901 cgtgttcaac agtcagatgg cacagcagct tagcctggaa ctgaatctcg ctgcaaagct
     3961 ctgtaacctc ctaaggaagt gcaaggaccg aaaatttatc aatgacataa agtgctttga
     4021 cttgcgcttg ctcttcgtct tgtcactttt gcacactgac atcaggtcac aattgcgcta
     4081 tgagctccag ggactcccgc tgctaaccca gatcttggaa agtgccttta gcatcaagtg
     4141 gaccgatgag tatgagtcgg ccatagacca taatggacct cctctctcac ctcaggagac
     4201 agactgtgcc attgaggccc tcaaagctct gttcaatgtg acggtagaca gttggaaggt
     4261 gcataaagag agcgattctc atcagtttcg tgtcatggca gctgtccttc gccattgttt
     4321 actaattgta ggtccaactg aagacaaaac cgaagagctg cacagtaatg ccgtcaacct
     4381 tttaagcaat gttccagtct cttgtttgga tgttctcatt tgtccattaa cccatgaaga
     4441 aacagcccaa gaggcagcaa ctctagatga actgcccagt gataaaacaa cggagaaaga
     4501 cacagctttg aaaaacagta ccatggtata caatggcatg aatatggagg ccattcacgt
     4561 tttactcaat tttatggaga agagaattga taagggaagc agctatagag agggtctaac
     4621 tccagttctc agcttattaa cagaatgttc ccgagcccat cggaacatcc gaaaatttct
     4681 caaagatcag gttttaccgc ctttgagaga tgtgacaaat cgaccagaag tcggctcaac
     4741 cgtgagaaat aagctggttc gtctcatgac acatgttgac ctcggagtca agcagattgc
     4801 tgctgagttc ctttttgtcc tttgcaaaga gagagtggat agcctgctga aatacactgg
     4861 ctatgggaac gctgcaggac tcttggcggc caggggcctc ttggctggag gaagaggaga
     4921 taattggtac tcagaggacg aggacacgga cactgaagaa tacaaaaacg caaaaccaaa
     4981 cattaatctt atcactggtc atttagagga accaatgcca aaccctatag atgaaatgac
     5041 agaagaacaa aaagaatatg aagccatgaa acttgtcaac atgcttgata aactttccag
     5101 agaggagttg cttagaccaa tgggactaaa acctgacggg acaataacgc ctttggagga
     5161 agcactcagc cagtattctg tcatcgaaga gaccagctcg gacacagact gaacgcgtct
     5221 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5281 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5341 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5401 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5461 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5521 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5581 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     5641 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5701 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5761 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     5821 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     5881 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     5941 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6001 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     6061 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     6121 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     6181 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     6241 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     6301 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     6361 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     6421 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     6481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6661 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6721 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     6781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8941 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9061 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     9121 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     9181 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9241 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     9301 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     9361 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     9421 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     9481 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     9541 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     9601 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     9661 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     9721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9901 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     9961 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10021 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10081 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10141 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10201 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10261 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10321 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10381 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
    10441 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10501 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10561 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10621 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    10681 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    10741 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    10801 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    10861 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    10921 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    10981 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    11041 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    11101 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    11161 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    11221 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
    11281 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    11341 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    11401 tcgggtttat tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt
    11461 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    11521 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    11581 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    11641 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    11701 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    11761 g
//
