LOCUS       sequence-372103-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-oA ONE-GO
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(715..717,718..720,721..1431)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(760..781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1021..1040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1368..1389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2168..2742,2743..2745,2746..2754)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2335..2352)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2562..2581
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2765..2785
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             2891..3406
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3420..4008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3473..3493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3891..3902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4215..4848
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4976..4992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4976..4992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4989..5011)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5000..5016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5024..5030,5031..5048,5049..5054))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5069..5090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5207..5224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5378..5966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5458..5477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6137..6928,6929..6997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6760..6779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6998..7102)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7150..7284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7187..7206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7241..7260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7452..8085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8132..8257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8754..8987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9172..9216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9479..9594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        9652..9955
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgg gatgtactct gagcgcagag
      361 gagagagccg ccctcgagcg gagcaaggcg attgagaaaa atctcaaaga agatggcatc
      421 agcgccgcca aagacgtgaa attactcctg ctgggggctg gagaatcagg aaaaagcacc
      481 attgtgaagc agatgaagat catccatgaa gatggcttct ctggagaaga cgtaaagcag
      541 tacaagcctg tcgtctacag caacaccatc cagtctctgg cagccattgt gcgggccatg
      601 gatactctgg gcgtggagta tggtgacaag gagaggaagg cagactccaa gatggtgtgt
      661 gacgtggtga gtcgcatgga ggacactgaa ccaagatccg gtgggggcgg gagcatggtg
      721 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac
      961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1201 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggaggt
     1441 ggaggatctt tctctgcaga actgctttct gccatgatgc gactctgggg cgactcgggg
     1501 atccaggagt gcttcaaccg atctcgggag tatcagctca acgactctgc caaatactac
     1561 ctggacagct tggatcggat tggagctgca gaatacatgc ccaccgagca ggacatcctc
     1621 cgaaccaggg tcaaaacaac tggcatcgta gaaacccact tcaccttcaa gaacctccac
     1681 ttcaggctgt ttgacgttgg gggccagcga tctgaacgta agaagtggat ccactgcttc
     1741 gaggatgtca cggccatcat cttctgtgtc gcactcagcg gctatgacca ggtgctccac
     1801 gaggacgaaa ccacgaaccg catgcacgag tctctcatgc tcttcgactc catctgtaac
     1861 aacaagtttt tcatcgatac ctccatcatt ctcttcctca acaagaaaga cctctttggc
     1921 gagaagatta agaagtcacc cttgaccatc tgctttcctg aatacccagg ctccaacacc
     1981 tatgaagacg cagctgccta catccaaaca cagtttgaaa gcaaaaaccg ctcacccaac
     2041 aaagaaattt actgtcacat gacttgtgcc acagacacga ataatatcca ggtggtattc
     2101 gacgccgtca ccgacatcat cattgccaac aatctccggg gctgtggctt gtactgagcg
     2161 gccgccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2221 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2281 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     2341 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     2401 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     2461 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     2521 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     2581 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     2641 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     2701 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgtacc
     2761 aaagatgggg agtagcaaga gcaagacgtc gaattccgga atcaagcttg gtggatcttc
     2821 tcgaggttac taccatggta tttgggtggg tgaagaaggt cgactttctc gaggtggcac
     2881 cggtggatcc atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     2941 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     3001 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     3061 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     3121 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     3181 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     3241 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     3301 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     3361 caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaaacgc gtctggaaca
     3421 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3481 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3541 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3601 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3661 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3721 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3781 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3841 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3901 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3961 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     4021 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4081 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4141 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4201 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4261 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     4321 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     4381 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     4441 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     4501 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     4561 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     4621 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     4681 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4741 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4801 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4861 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     4921 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     4981 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5041 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5101 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5161 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5221 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5281 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6001 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6061 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6121 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6181 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6301 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6361 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6421 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6481 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6541 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6601 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6661 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6721 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6781 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6841 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6901 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6961 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7021 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7081 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7141 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7201 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7261 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     7321 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     7381 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     7441 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     7501 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7561 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7621 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7681 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7741 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7801 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7861 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8101 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8161 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8221 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8281 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8341 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8401 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8461 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8521 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8581 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8641 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8701 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8761 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8821 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8881 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8941 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9001 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9061 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9121 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9181 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9241 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9301 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9361 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9421 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgcctt
     9481 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9541 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9601 ttattacagg gacagcagag atccagttta tcgataagct tgggagttcc gcgttacata
     9661 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9721 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9781 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     9841 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     9901 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
