LOCUS       sequence-372106-        9958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-z ONE-GO
FEATURES             Location/Qualifiers
     source          1..9958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(715..717,718..720,721..1431)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(760..781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1021..1040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1368..1389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2171..2745,2746..2748,2749..2757)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2338..2355)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2565..2584
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2768..2788
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             2894..3409
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3423..4011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3476..3496)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3894..3905)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4218..4851
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4979..4995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4979..4995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4992..5014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5003..5019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5027..5033,5034..5051,5052..5057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5072..5093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5210..5227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5381..5969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5461..5480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6140..6931,6932..7000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6763..6782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7001..7105)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7153..7287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7190..7209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7244..7263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7455..8088
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8135..8260
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8757..8990
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9175..9219
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9482..9597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        9655..9958
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgg gctgtcggca gtcatcagaa
      361 gaaaaggagg cggcacgacg atctaggcgc atagatcgcc atctgcgatc tgaatcacag
      421 cggcaaaggc gcgaaattaa gcttctcttg ctgggaacct ccaattctgg caaaagcaca
      481 atcgtaaaac aaatgaagat aattcattca ggcggtttta atctcgaggc ctgcaaggag
      541 tataaaccct tgataatata taacgccata gattcactca cacgaattat ccgggctctg
      601 gcagcgctta aaattgactt ccacaatcct gaccgcgcat acgatgctgt acaactgttt
      661 gcattgaccg gcccggctga gtcaaaaggt gaacgatctg gaggtggggg ctctatggtg
      721 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag tgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac
      961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1081 cgcattgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1201 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtctggcggt
     1441 ggaggctcaa ttaccccgga gcttctggga gtcatgagga ggctctgggc cgaccccgga
     1501 gcacaagcat gcttcgggcg gagttcagaa tatcacctgg aagataatgc tgcgtattat
     1561 ttgaatgact tggaacgaat tgctgctcct gattatatcc cgaccgtgga ggacattttg
     1621 cggtctaggg acatgaccac agggattgtc gagaacaaat tcacgtttaa ggaacttaca
     1681 ttcaaaatgg tcgatgtggg tggacagcgg tctgagcgga aaaagtggat acattgcttc
     1741 gagggggtaa cggccattat cttttgtgtt gaactctctg gttacgatct gaaattgtac
     1801 gaggacaacc agacgtcccg aatggccgaa agtctccgct tgtttgacag catttgcaac
     1861 aataactggt ttattaacac ttcactcata ctttttctca acaagaagga ccttctgagt
     1921 gaaaaaatac gacgcattcc gcttagtgtg tgctttccgg agtacaaggg gcagaacact
     1981 tatgaggagg ctgccgtgta cattcagcgg caattcgagg acctcaacag gaataaggag
     2041 acaaaagaaa tttactccca ttttacttgt gcgaccgaca cttccaatat tcagtttgtc
     2101 tttgacgctg tcaccgacgt cattatccag aataacctta agtacatagg gttgtgctga
     2161 gcggccgccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     2221 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2281 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2341 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2401 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2461 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2521 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2581 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2641 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     2701 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgt
     2761 accaaagatg gggagtagca agagcaagac gtcgaattcc ggaatcaagc ttggtggatc
     2821 ttctcgaggt tactaccatg gtatttgggt gggtgaagaa ggtcgacttt ctcgaggtgg
     2881 caccggtgga tccatggtct tcacactcga agatttcgtt ggggactggc gacagacagc
     2941 cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct
     3001 cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat
     3061 cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga
     3121 aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta
     3181 tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta
     3241 tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg
     3301 caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac
     3361 catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaaa cgcgtctgga
     3421 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3481 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3541 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3601 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3661 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3721 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3781 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3841 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3901 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3961 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     4021 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     4081 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     4141 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4201 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     4261 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     4321 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     4381 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     4441 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     4501 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     4561 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     4621 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     4681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     4861 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4921 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     4981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     7141 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7201 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7261 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     7321 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     7381 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     7441 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     7501 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     7561 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     7621 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     7681 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     7741 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     7801 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     7861 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     7921 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7981 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8041 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8101 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8161 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8221 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8281 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8341 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8401 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8461 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8521 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     8581 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     8641 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8701 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8761 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8821 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8881 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8941 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9001 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9061 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9121 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9181 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9241 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9301 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9361 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9421 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     9481 ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     9541 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9601 ggtttattac agggacagca gagatccagt ttatcgataa gcttgggagt tccgcgttac
     9661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     9721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     9781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     9841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     9901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
