LOCUS       sequence-372121-        3298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty backbone with chloramphenicol resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pSKA322
FEATURES             Location/Qualifiers
     source          1..3298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..138
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(686..1636)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1684..1906
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2398..2492)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2513..3172)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3087..3106
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3173..3275)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcgttg ttggcgcgcc caacaattaa ttaagaagaa tctagaggca tcaaataaaa
       61 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      121 ctcctgagta ggacaaatcc gccgccctag acctagctgc agctagggta cgggttttgc
      181 tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt
      241 cagccggttt gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac
      301 gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg
      361 tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc
      421 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc
      481 atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa
      541 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
      601 ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
      661 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
      721 gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt
      781 gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat
      841 cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta
      901 ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc
      961 tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca
     1021 atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa
     1081 gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc
     1141 taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat
     1201 aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga
     1261 atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt
     1321 cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat
     1381 tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag
     1441 cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc
     1501 gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta
     1561 gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa
     1621 caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga
     1681 gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat
     1741 tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta
     1801 gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag
     1861 aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag
     1921 ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt
     1981 aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt
     2041 ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg
     2101 ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat
     2161 gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg
     2221 gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat
     2281 tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc
     2341 cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt
     2401 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     2461 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     2521 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     2581 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     2641 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     2701 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     2761 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     2821 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     2881 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     2941 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     3001 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     3061 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     3121 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     3181 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     3241 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc cagatatc
//
