LOCUS       sequence-372196-         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expresses human talin R8 domain flanked between a HaloTag 
            and a SpyTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Dahal N, Sharma S, Phan B, Eis A, Popa I
  TITLE     Mechanical regulation of talin through binding and history-dependent
            unfolding.
  JOURNAL   Sci Adv. 2022 Jul 15;8(28):eabl7719. doi: 10.1126/sciadv.abl7719. 
            Epub 2022 Jul 15.
  PUBMED    35857491
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-07-15"; volume: "8(28):eabl7719. doi"; pages: " 
            10.1126/sciadv.abl7719. Epub 2022 Jul 15"
COMMENT     Sequence Label: pFN-HaloTag-TalinR8-SpyTag
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             421..459
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             475..492
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcgataacg cgatcgccgg atccgccggt caacagggtc tggttgaacc gacccagttt
       61 gcccgcgcca atcaggccat ccagatggca tgccagagct taggtgagcc gggttgcacc
      121 caggcccagg ttctgagcgc cgcaaccatt gttgccaaac ataccagcgc cctgtgtaat
      181 agctgccgcc tggcaagcgc ccgtaccaca aatccgaccg ccaaacgcca gttcgtgcag
      241 agcgccaaag aagtggccaa tagcacagcc aacctggtta aaaccatcaa ggccctggac
      301 ggtgcattta ccgaggaaaa tcgcgcacag tgtcgcgcag caacagcccc gctgctggaa
      361 gcagttgaca acctgagcgc cttcgccagc aatcctgagt ttagcagcat tccgagatcc
      421 gcccacatcg tgatggtgga cgcctacaag ccgacgaagg ggggttgtag atcccaccat
      481 caccatcacc attgctaagg taccagat
//
