LOCUS       sequence-372208-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human FLT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_FLT1
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(139..156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(310..898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(390..409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1069..1860,1861..1929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1692..1711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1930..2034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2109..2128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2300..2679
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2680..2883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2834..2854
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2885..2903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2906..2924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2934..2958
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3101..3125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7146..7170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7217..7240
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7277..7300
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7331..7357
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7331..7357
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7358..7705,7706..7708,7709..8317)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8361..8378)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8367..8591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8724..8743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8875..8922
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8930..9952
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8930..9952
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10082..10203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10119..10138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10173..10192
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10252..10268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10252..10268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10265..10287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10276..10292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10300..10306,10307..10324,10325..10330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
       61 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2041 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     2101 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     2161 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     2221 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     2281 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2341 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2401 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2461 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2521 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2581 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2641 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2701 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2761 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2821 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2881 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     2941 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3001 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
     3061 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     3121 aggctccacc atggtcagct actgggacac cggggtcctg ctgtgcgcgc tgctcagctg
     3181 tctgcttctc acaggatcta gttcaggttc aaaattaaaa gatcctgaac tgagtttaaa
     3241 aggcacccag cacatcatgc aagcaggcca gacactgcat ctccaatgca ggggggaagc
     3301 agcccataaa tggtctttgc ctgaaatggt gagtaaggaa agcgaaaggc tgagcataac
     3361 taaatctgcc tgtggaagaa atggcaaaca attctgcagt actttaacct tgaacacagc
     3421 tcaagcaaac cacactggct tctacagctg caaatatcta gctgtaccta cttcaaagaa
     3481 gaaggaaaca gaatctgcaa tctatatatt tattagtgat acaggtagac ctttcgtaga
     3541 gatgtacagt gaaatccccg aaattataca catgactgaa ggaagggagc tcgtcattcc
     3601 ctgccgggtt acgtcaccta acatcactgt tactttaaaa aagtttccac ttgacacttt
     3661 gatccctgat ggaaaacgca taatctggga cagtagaaag ggcttcatca tatcaaatgc
     3721 aacgtacaaa gaaatagggc ttctgacctg tgaagcaaca gtcaatgggc atttgtataa
     3781 gacaaactat ctcacacatc gacaaaccaa tacaatcata gatgtccaaa taagcacacc
     3841 acgcccagtc aaattactta gaggccatac tcttgtcctc aattgtactg ctaccactcc
     3901 cttgaacacg agagttcaaa tgacctggag ttaccctgat gaaaaaaata agagagcttc
     3961 cgtaaggcga cgaattgacc aaagcaattc ccatgccaac atattctaca gtgttcttac
     4021 tattgacaaa atgcagaaca aagacaaagg actttatact tgtcgtgtaa ggagtggacc
     4081 atcattcaaa tctgttaaca cctcagtgca tatatatgat aaagcattca tcactgtgaa
     4141 acatcgaaaa cagcaggtgc ttgaaaccgt agctggcaag cggtcttacc ggctctctat
     4201 gaaagtgaag gcatttccct cgccggaagt tgtatggtta aaagatgggt tacctgcgac
     4261 tgagaaatct gctcgctatt tgactcgtgg ctactcgtta attatcaagg acgtaactga
     4321 agaggatgca gggaattata caatcttgct gagcataaaa cagtcaaatg tgtttaaaaa
     4381 cctcactgcc actctaattg tcaatgtgaa accccagatt tacgaaaagg ccgtgtcatc
     4441 gtttccagac ccggctctct acccactggg cagcagacaa atcctgactt gtaccgcata
     4501 tggtatccct caacctacaa tcaagtggtt ctggcacccc tgtaaccata atcattccga
     4561 agcaaggtgt gacttttgtt ccaataatga agagtccttt atcctggatg ctgacagcaa
     4621 catgggaaac agaattgaga gcatcactca gcgcatggca ataatagaag gaaagaataa
     4681 gatggctagc accttggttg tggctgactc tagaatttct ggaatctaca tttgcatagc
     4741 ttccaataaa gttgggactg tgggaagaaa cataagcttt tatatcacag atgtgccaaa
     4801 tgggtttcat gttaacttgg aaaaaatgcc gacggaagga gaggacctga aactgtcttg
     4861 cacagttaac aagttcttat acagagacgt tacttggatt ttactgcgga cagttaataa
     4921 cagaacaatg cactacagta ttagcaagca aaaaatggcc atcactaagg agcactccat
     4981 cactcttaat cttaccatca tgaatgtttc cctgcaagat tcaggcacct atgcctgcag
     5041 agccaggaat gtatacacag gggaagaaat cctccagaag aaagaaatta caatcagaga
     5101 tcaggaagca ccatacctcc tgcgaaacct cagtgatcac acagtggcca tcagcagttc
     5161 caccacttta gactgtcatg ctaatggtgt ccccgagcct cagatcactt ggtttaaaaa
     5221 caaccacaaa atacaacaag agcctggaat tattttagga ccaggaagca gcacgctgtt
     5281 tattgaaaga gtcacagaag aggatgaagg tgtctatcac tgcaaagcca ccaaccagaa
     5341 gggctctgtg gaaagttcag catacctcac tgttcaagga acctcggaca agtctaatct
     5401 ggagctgatc actctaacat gcacctgtgt ggctgcgact ctcttctggc tcctattaac
     5461 cctctttatc cgaaaaatga aaaggtcttc ttctgaaata aagactgact acctatcaat
     5521 tataatggac ccagatgaag ttcctttgga tgagcagtgt gagcggctcc cttatgatgc
     5581 cagcaagtgg gagtttgccc gggagagact taaactgggc aaatcacttg gaagaggggc
     5641 ttttggaaaa gtggttcaag catcagcatt tggcattaag aaatcaccta cgtgccggac
     5701 tgtggctgtg aaaatgctga aagagggggc cacggccagc gagtacaaag ctctgatgac
     5761 tgagctaaaa atcttgaccc acattggcca ccatctgaac gtggttaacc tgctgggagc
     5821 ctgcaccaag caaggagggc ctctgatggt gattgttgaa tactgcaaat atggaaatct
     5881 ctccaactac ctcaagagca aacgtgactt attttttctc aacaaggatg cagcactaca
     5941 catggagcct aagaaagaaa aaatggagcc aggcctggaa caaggcaaga aaccaagact
     6001 agatagcgtc accagcagcg aaagctttgc gagctccggc tttcaggaag ataaaagtct
     6061 gagtgatgtt gaggaagagg aggattctga cggtttctac aaggagccca tcactatgga
     6121 agatctgatt tcttacagtt ttcaagtggc cagaggcatg gagttcctgt cttccagaaa
     6181 gtgcattcat cgggacctgg cagcgagaaa cattctttta tctgagaaca acgtggtgaa
     6241 gatttgtgat tttggccttg cccgggatat ttataagaac cccgattatg tgagaaaagg
     6301 agatactcga cttcctctga aatggatggc tcccgaatct atctttgaca aaatctacag
     6361 caccaagagc gacgtgtggt cttacggagt attgctgtgg gaaatcttct ccttaggtgg
     6421 gtctccatac ccaggagtac aaatggatga ggacttttgc agtcgcctga gggaaggcat
     6481 gaggatgaga gctcctgagt actctactcc tgaaatctat cagatcatgc tggactgctg
     6541 gcacagagac ccaaaagaaa ggccaagatt tgcagaactt gtggaaaaac taggtgattt
     6601 gcttcaagca aatgtacaac aggatggtaa agactacatc ccaatcaatg ccatactgac
     6661 aggaaatagt gggtttacat actcaactcc tgccttctct gaggacttct tcaaggaaag
     6721 tatttcagct ccgaagttta attcaggaag ctctgatgat gtcagatatg taaatgcttt
     6781 caagttcatg agcctggaaa gaatcaaaac ctttgaagaa cttttaccga atgccacctc
     6841 catgtttgat gactaccagg gcgacagcag cactctgttg gcctctccca tgctgaagcg
     6901 cttcacctgg actgacagca aacccaaggc ctcgctcaag attgacttga gagtaaccag
     6961 taaaagtaag gagtcggggc tgtctgatgt cagcaggccc agtttctgcc attccagctg
     7021 tgggcacgtc agcgaaggca agcgcaggtt cacctacgac cacgctgagc tggaaaggaa
     7081 aatcgcgtgc tgctccccgc ccccagacta caactcggtg gtcctgtact ccaccccacc
     7141 catcgaccca gctttcttgt acaaagtggt tgatatcaag gccgccgaca tcaccagcct
     7201 gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat ctggcggcgg
     7261 aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc tgggcagcac
     7321 catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg tgcccctgaa
     7381 gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg gcgagacact
     7441 gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact ggggcgtgga
     7501 cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc tgctgaacgc
     7561 caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg tgatcgacag
     7621 caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg cctgtatcgc
     7681 cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc ccttcggcgc
     7741 caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg ctattggcct
     7801 gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt
     7861 gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg gcatcctggt
     7921 ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat
     7981 ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc tgcaggaagc
     8041 cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc tgagagccgc
     8101 cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg agaagctgga
     8161 caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct tcggcatcag
     8221 ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca tcaagccctg
     8281 gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt ctagagggcc
     8341 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8401 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8461 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8521 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8581 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8641 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8701 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8761 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8821 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt
     8881 cctattccga agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga
     8941 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     9001 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     9061 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     9121 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     9181 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     9241 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     9301 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     9361 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     9421 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     9481 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     9541 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     9601 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     9661 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     9721 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     9781 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     9841 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     9901 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact
     9961 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10021 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10081 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10141 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10201 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    10261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10321 aaagtgtaaa gcctggggtg ccta
//
